LEPRE1 Antibody (3C7) - Azide and BSA Free

Novus Biologicals | Catalog # H00064175-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3C7

Format

Azide and BSA Free
View all LEPRE1 Primary Antibodies »

Product Specifications

Immunogen

LEPRE1 (AAH15309, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DPRVREVMNQNLAYYAAMLGEEHTRSIGPRESAKEYRQRSLLEKELLFFAYDVFGIPFVDPDSWTPEEVIPKRLQEKQKSERETAVRISQEIGNLMKEIE

Specificity

LEPRE1 - leucine proline-enriched proteoglycan (leprecan) 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for LEPRE1 Antibody (3C7) - Azide and BSA Free

Western Blot: LEPRE1 Antibody (3C7) [H00064175-M01]

Western Blot: LEPRE1 Antibody (3C7) [H00064175-M01]

Western Blot: LEPRE1 Antibody (3C7) [H00064175-M01] - LEPRE1 monoclonal antibody (M01), clone 3C7 Analysis of LEPRE1 expression in HeLa.
Immunocytochemistry/ Immunofluorescence: LEPRE1 Antibody (3C7) [H00064175-M01]

Immunocytochemistry/ Immunofluorescence: LEPRE1 Antibody (3C7) [H00064175-M01]

Immunocytochemistry/Immunofluorescence: LEPRE1 Antibody (3C7) [H00064175-M01] - Analysis of monoclonal antibody to LEPRE1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: LEPRE1 Antibody (3C7) [H00064175-M01]

Immunohistochemistry-Paraffin: LEPRE1 Antibody (3C7) [H00064175-M01]

Immunohistochemistry-Paraffin: LEPRE1 Antibody (3C7) [H00064175-M01] - Analysis of monoclonal antibody to LEPRE1 on formalin-fixed paraffin-embedded human esophagus. Antibody concentration 6 ug/ml.
Western Blot: LEPRE1 Antibody (3C7) [H00064175-M01]

Western Blot: LEPRE1 Antibody (3C7) [H00064175-M01]

Western Blot: LEPRE1 Antibody (3C7) [H00064175-M01] - Analysis of LEPRE1 expression in transfected 293T cell line by LEPRE1 monoclonal antibody (M01), clone 3C7.Lane 1: LEPRE1 transfected lysate(46 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: LEPRE1 Antibody (3C7) [H00064175-M01] - Detection limit for recombinant GST tagged LEPRE1 is approximately 0.3ng/ml as a capture antibody.

Applications for LEPRE1 Antibody (3C7) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recominant protein, transfected lysate and cell lysate for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA. Reactivity with Mouse species is reported in scientific literature (PMID 19997487)

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: LEPRE1

This gene encodes an enzyme that is a member of the collagen prolyl hydroxylase family. These enzymes are localized to the endoplasmic reticulum and their activity is required for proper collagen synthesis and assembly. Mutations in this gene are associated with osteogenesis imperfecta type VIII. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.

Alternate Names

EC 1.14.11.7, GROS1OI8, Growth suppressor 1, leprecan, Leprecan-1, Leucine- and proline-enriched proteoglycan 1, leucine proline-enriched proteoglycan (leprecan) 1, MGC117314, P3H1LEPRECAN, prolyl 3-hydroxylase 1

Entrez Gene IDs

64175 (Human)

Gene Symbol

P3H1

UniProt

AAH15309 (Human)

Additional LEPRE1 Products

Product Documents for LEPRE1 Antibody (3C7) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for LEPRE1 Antibody (3C7) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for LEPRE1 Antibody (3C7) - Azide and BSA Free

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Protocols

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