Leptin/OB Antibody (3G7) - BSA Free
Novus Biologicals | Catalog # NB110-8268
Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA, Sandwich ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 3G7
Format
BSA Free
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Product Specifications
Immunogen
Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with recombinant leptin.
Reactivity Notes
May be reactive to mouse and rat leptin, as human leptin is 85% and 84% identical respectively.
Localization
Secreted
Specificity
Recognizes recombinant human leptin and leptin circulating in human blood.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Leptin/OB Antibody (3G7) - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Western Blot
1:100-1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Leptin/OB
Additional Leptin/OB Products
Product Documents for Leptin/OB Antibody (3G7) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Leptin/OB Antibody (3G7) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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