Lgr5/GPR49 Antibody (CL13579) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-43804

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL13579

Format

Azide and BSA Free
View all Lgr5/GPR49 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of O75473, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Lgr5/GPR49 Antibody (CL13579) - Azide and BSA Free

Lgr5/GPR49 Antibody (CL13579) - Azide and BSA Free Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody [NBP3-43804]

Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody [NBP3-43804]

Analysis in human placenta and tonsil tissues using NBP3-43804 antibody. Corresponding Lgr5/GPR49 RNA-seq data are presented for the same tissues.
Lgr5/GPR49 Antibody (CL13579) - Azide and BSA Free Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody [NBP3-43804]

Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody [NBP3-43804]

Staining of human duodenum shows moderate cytoplasmic and membranous positivity in glandular cells.
Lgr5/GPR49 Antibody (CL13579) - Azide and BSA Free Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody [NBP3-43804]

Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody [NBP3-43804]

Staining of human cerebral cortex shows moderate cytoplasmic and membranous positivity in neurons and neuropil.
Lgr5/GPR49 Antibody (CL13579) - Azide and BSA Free Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody [NBP3-43804]

Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody [NBP3-43804]

Staining of human colorectal cancer shows moderate cytoplasmic positivity in tumor cells.
Lgr5/GPR49 Antibody (CL13579) - Azide and BSA Free Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody [NBP3-43804]

Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody [NBP3-43804]

Staining of human ovarian cancer (endometrioid carcinoma) shows moderate cytoplasmic positivity in tumor cells.
Lgr5/GPR49 Antibody (CL13579) - Azide and BSA Free Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody [NBP3-43804]

Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody [NBP3-43804]

Staining of human ovarian cancer (papillary carcinoma) shows moderate to strong cytoplasmic and membranous positivity in tumor cells.
Lgr5/GPR49 Antibody (CL13579) - Azide and BSA Free Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody [NBP3-43804]

Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody [NBP3-43804]

Staining of human placenta shows moderate cytoplasmic and membranous positivity in trophoblastic cells.
Lgr5/GPR49 Antibody (CL13579) - Azide and BSA Free Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody [NBP3-43804]

Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody [NBP3-43804]

Staining of human tonsil shows mainly very weak positivity in non-germinal center cells.

Applications for Lgr5/GPR49 Antibody (CL13579) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Lgr5/GPR49

Leucine-rich repeat-containing LGR5, also known as GPR49, is an Orphan-A G-protein coupled receptor with an unknown ligand. GPR49 is a stem cell marker of the intestinal epithelium and the hair follicule, and is often expressed in the brain, skeletal muscle, placenta, and spinal cord. Because LGR5 is typically found in mouse hair follicles it may potentially be used to regrow hair in mice. GPR49/LGR5 antibodies are useful tools for research on hair loss and hair follicule development.

Long Name

Leucine-rich Repeat Containing G Protein-coupled Receptor 5

Alternate Names

FEX, GPR49, GPR67, HG38

Gene Symbol

LGR5

Additional Lgr5/GPR49 Products

Product Documents for Lgr5/GPR49 Antibody (CL13579) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Lgr5/GPR49 Antibody (CL13579) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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