Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

DyLight 650 (Excitation = 652 nm, Emission = 672 nm)

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

Synthetic peptide [KLH conjugated] made to the 2nd cytoplasmic loop of human GPR49/LGR5. [UniProt# O75473]

Reactivity Notes

Immunogen sequence has 81% identity with mouse.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for Lgr5/GPR49 Antibody [DyLight 650]

Application
Recommended Usage

Immunohistochemistry

5-8 ug/ml

Immunohistochemistry-Paraffin

5-8 ug/ml
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Lgr5/GPR49

GPR49 is an Orphan-A GPCR with an unknown ligand. G protein-coupled receptor GPR49 has been reported to be expressed in brain, skeletal muscle, placenta, and spinal cord. ESTs have been isolated from normal human brain (amygdala), embryo, and placenta libraries and from human germ cell, uterus, and brain (neuroblastoma) cancer libraries.

Long Name

Leucine-rich Repeat Containing G Protein-coupled Receptor 5

Alternate Names

FEX, GPR49, GPR67, HG38

Gene Symbol

LGR5

Additional Lgr5/GPR49 Products

Product Documents for Lgr5/GPR49 Antibody [DyLight 650]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Lgr5/GPR49 Antibody [DyLight 650]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Lgr5/GPR49 Antibody [DyLight 650]

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  • Q: Can you confirm whether or not NLS1235C is supported for use in ICC application? Has it been tested in ICC and shown to be ineffective?

    A: Unfortunately, neither the base antibody NLS1235 nor NLS1235C has been tested in ICC/IF. Therefore we don't know whether it will work in ICC. This series of the anybodies with the conjugation formats have only been validated in IHC-P with the human specimens. If you would like to test an antibody in an untested species and/or application and share your results with us, I can recommend our Innovator's Reward program. Our Innovator's Reward program was created to allow researchers the opportunity to try our primary antibodies in an untested species or application, without the financial risk of failure. To participate you simply submit an online review detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Reviews may also be submitted by emailing Innovators Reward.

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