Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Lgr5/GPR49 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide [KLH conjugated] made to the 2nd cytoplasmic loop of human GPR49/LGR5. [UniProt# O75473]

Reactivity Notes

Immunogen sequence has 81% identity with mouse.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Lgr5/GPR49 Antibody - Azide and BSA Free

Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody - Azide and BSA Free [NLS1235]

Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody - Azide and BSA Free [NLS1235]

Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody [NLS1235] - GPR49/LGR5 Antibody [NLS1235] - Staining of a FFPE section of human cortex.
Immunohistochemistry: Lgr5/GPR49 Antibody - Azide and BSA Free [NLS1235]

Immunohistochemistry: Lgr5/GPR49 Antibody - Azide and BSA Free [NLS1235]

Immunohistochemistry: Lgr5/GPR49 Antibody [NLS1235] - Stem cells, neutrophils and eosinophils in human bone marrow (1ug/ml).

Applications for Lgr5/GPR49 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

5-8 ug/ml

Immunohistochemistry-Paraffin

5-8 ug/ml
Application Notes
This GPR49/LGR5 antibody is useful for Immunohistochemistry on paraffin sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lgr5/GPR49

GPR49/LGR5 (G protein-coupled receptor 49 / leucine-rich repeat-containing G protein-coupled receptor 5), also called FEX, GPR67, GRP49, HG38 or MGC117008), is structurally related to members of glycoprotein hormone receptor family including FSH-R, TSH-R and LH-R. LGRs (leucine-rich repeat-containing G-protein coupled receptor) are divided into three major subgroups: glycoprotein hormone receptors; the subgroup of GPR48, GPR49, and LGR6; and receptors of relaxin family ligands (LGR7 and LGR8), of which GPR49 is emerging as a significant player in stem cells biology as it is highly expressed on stem cells in small intestine, colon and hair follicle. Deficiency of GPR49 in mice has been shown to cause neonatal lethality characterized by ankyloglossia, gastrointestinal distension and induction of premature differentiation of Paneth cells. LGR5 is a downstream target gene of Wnt signaling pathway (critical for morphogenesis and tumorigenesis) and it has also been reported as a receptor for R-spondins which are potent mediators of Wnt/beta-catenin as well as Wnt/PCP signaling. Accordingly, when compared to normal tissues, LGR5 is found upregulated in certain cancers e.g liver cancer with beta-catenin mutations, basal cell carcinoma, colon and ovarian carcinomas.

Long Name

Leucine-rich Repeat Containing G Protein-coupled Receptor 5

Alternate Names

FEX, GPR49, GPR67, HG38

Entrez Gene IDs

8549 (Human)

Gene Symbol

LGR5

UniProt

O75473 (Human)

Additional Lgr5/GPR49 Products

Product Documents for Lgr5/GPR49 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Lgr5/GPR49 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Lgr5/GPR49 Antibody - Azide and BSA Free

There are currently no reviews for this product. Be the first to review Lgr5/GPR49 Antibody - Azide and BSA Free and earn rewards!

Have you used Lgr5/GPR49 Antibody - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

View specific protocols for Lgr5/GPR49 Antibody - Azide and BSA Free (NLS1235):

Lgr5/GPR49 Antibody:
Immunohistochemistry

1. Prepare tissue with formalin fixation and by embedding it in paraffin wax.

2. Make 4-mm sections and place on pre-cleaned and charged microscope slides.

3. Heat in a tissue-drying oven for 45 minutes @ 60 degrees Celcius.

4. Deparaffinize the tissues by wash drying the slides in 3 changes of xylene 5 minutes each @ RT.

5. Rehydrate the tissues by washing the slides in 3 changes of 100% alcohol 3 minutes each @ RT.

6. Wash the slides in 2 changes of 95% alcohol 3 minutes each @ RT.

7. Wash the slides in 1 change of 80% alcohol 3 minutes @ RT.

8. Rinse the slides in gentle running distilled water 5 minutes @ RT.

9. Perform antigen retrieval by steaming the slides in 0.01M sodium citrate buffer (pH 6.0) @ 99-100 degrees Celcius for 20 minutes.

10. Remove the slides from the heat and let stand in buffer @ RT for 20 minutes.

11. Rinse the slides in 1X TBS-T for 1 minute @ RT.

**Do not allow the tissues to dry at any time during the staining procedure**

12. Begin the immunostaining by applying a universal protein block 20 minutes @ RT.

13. Drain protein block from the slides and apply the diluted primary antibody 45 minutes @ RT.

14. Rinse the slide in 1X TBS-T 1 minute @ RT.

15. Apply a biotinylated anti-rabbit IgG (H+L) secondary 30 minutes @ RT.

16. Rinse the slide in 1X TBS-T 1 minute @ RT.

17. Apply an alkaline phosphatase steptavidin 30 minutes @ RT.

18. Rinse the slide in 1X TBS-T 1 minute @ RT.

19. Apply an alkaline phosphatase chromagen substrate 30 minutes @ RT.

20. Rinse the slide in distilled water 1 minute @ RT.

**This method should only be used if the chromagen substrate is alcohol insoluble (ie: Vector Red, DAB)**

21. Dehydrate the tissue by washing the slides in 2 changes of 80% alcohol 1 minute each @ RT.

22. Wash the slides in 2 changes of 95% alcohol 1 minute each @ RT.

23. Wash the slides in 3 changes of 100% alcohol 1 minute each @ RT.

24. Wash the slides in 3 changes of xylene 1 minute each @ RT.

25. Apply cover slip.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies