LIMPII/SR-B2 Antibody - BSA Free

Novus Biologicals | Catalog # NB400-129

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Primate

Cited:

Human, Mouse, Rat, Canine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all LIMPII/SR-B2 Primary Antibodies »

Product Specifications

Immunogen

A C-terminal synthetic peptide made to the mouse LIMPII/lgp85 protein sequence. [UniProt# O35114]

Localization

Lysosome membrane; Multi-pass membrane protein.

Marker

Lysosome Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LIMPII/SR-B2 Antibody - BSA Free

Immunohistochemistry-Paraffin: LIMPII/SR-B2 Antibody [NB400-129]

Immunohistochemistry-Paraffin: LIMPII/SR-B2 Antibody [NB400-129]

LIMPII-SR-B2-Antibody-Immunohistochemistry-Paraffin-NB400-129-img0009.jpg
Western Blot: LIMPII/SR-B2 Antibody [NB400-129]

Western Blot: LIMPII/SR-B2 Antibody [NB400-129]

Western Blot: LIMPII/SR-B2 Antibody [NB400-129] - Detection of LIMP II (~85 kDa) in Lane 1: Rat cardiac fibroblasts (RCF), 3T3 mouse fibroblasts (2), canine lung lysate (3), RCF treated with angiotensin II (4), and H2O2 (5), HEPG2 human liver lysate (6).
Simple Western: LIMPII/SR-B2 Antibody [NB400-129]

Simple Western: LIMPII/SR-B2 Antibody [NB400-129]

Simple Western: LIMPII/SR-B2 Antibody [NB400-129] - CHAPS-solublized SVG-A (human astrocyte cells) and B103 (rat neuroblastoma cells) cell lyates, at the indicated concentrations, were probed with a 1:25 dilution of antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: LIMPII/SR-B2 Antibody [NB400-129]

Immunohistochemistry-Paraffin: LIMPII/SR-B2 Antibody [NB400-129]

LIMPII-SR-B2-Antibody-Immunohistochemistry-Paraffin-NB400-129-img0010.jpg
Immunocytochemistry/ Immunofluorescence: LIMPII/SR-B2 Antibody [NB400-129]

Immunocytochemistry/ Immunofluorescence: LIMPII/SR-B2 Antibody [NB400-129]

Immunocytochemistry/Immunofluorescence: LIMPII/SR-B2 Antibody [NB400-129] - Detection of LIMPII/lpg85 (Green) in Hela cells using NB400-129. Nuclei (Blue) are counterstained using Hoechst 33258.
Immunohistochemistry: LIMPII/SR-B2 Antibody [NB400-129]

Immunohistochemistry: LIMPII/SR-B2 Antibody [NB400-129]

LIMPII-SR-B2 Antibody-Immunohistochemistry-NB400-129-img0011.jpg
Immunohistochemistry: LIMPII/SR-B2 Antibody [NB400-129]

Immunohistochemistry: LIMPII/SR-B2 Antibody [NB400-129]

Immunohistochemistry: LIMPII/SR-B2 Antibody [NB400-129] - Staining of LIMP II in rat infarctzone tissue with a DAB detection system.
LIMPII/SR-B2 Antibody

Immunocytochemistry/ Immunofluorescence: LIMPII/SR-B2 Antibody [NB400-129] -

Immunocytochemistry/ Immunofluorescence: LIMPII/SR-B2 Antibody [NB400-129] - Comparison of LIMP2-positive organelles in young (A), adult (B), & aged (C) rat urothelium. LIMP2-positive structures (green) shown in umbrella cells (delineated by dashed yellow line). Rhodamine phalloidin (red), which labels the cortical actin cytoskeleton, was used to show cell borders. Umbrella cells show an apparent, age-related increase in LIMP2 staining (white arrows). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29883476), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
LIMPII/SR-B2 Antibody

Immunocytochemistry/ Immunofluorescence: LIMPII/SR-B2 Antibody [NB400-129] -

Immunocytochemistry/ Immunofluorescence: LIMPII/SR-B2 Antibody [NB400-129] - Lysotracker Red accumulates in endolysosomes.Immunofluorescence of young (A), adult (B), & aged (C) urothelium incubated with Lysotracker Red (red) & co-stained with antibodies to LIMP2 (green structures) & phalloidin (blue), which labels the cortical actin cytoskeleton. Large, Lysotracker Red-positive endolysosomes are marked with white arrows. Underlying intermediate (IC) & basal cells (BC) show non-specific cytoplasmic Lysotracker Red staining that did not appear in umbrella cells (UC). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29883476), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
LIMPII/SR-B2 Antibody

Immunocytochemistry/ Immunofluorescence: LIMPII/SR-B2 Antibody [NB400-129] -

Immunocytochemistry/ Immunofluorescence: LIMPII/SR-B2 Antibody [NB400-129] - Comparison of LIMP2-positive organelles in young (A), adult (B), & aged (C) rat urothelium. LIMP2-positive structures (green) shown in umbrella cells (delineated by dashed yellow line). Rhodamine phalloidin (red), which labels the cortical actin cytoskeleton, was used to show cell borders. Umbrella cells show an apparent, age-related increase in LIMP2 staining (white arrows). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29883476), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
LIMPII/SR-B2 Antibody

Immunocytochemistry/ Immunofluorescence: LIMPII/SR-B2 Antibody [NB400-129] -

Immunocytochemistry/ Immunofluorescence: LIMPII/SR-B2 Antibody [NB400-129] - Comparison of LIMP2-positive organelles in young (A), adult (B), & aged (C) rat urothelium. LIMP2-positive structures (green) shown in umbrella cells (delineated by dashed yellow line). Rhodamine phalloidin (red), which labels the cortical actin cytoskeleton, was used to show cell borders. Umbrella cells show an apparent, age-related increase in LIMP2 staining (white arrows). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29883476), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
LIMPII/SR-B2 Antibody

Immunocytochemistry/ Immunofluorescence: LIMPII/SR-B2 Antibody [NB400-129] -

Immunocytochemistry/ Immunofluorescence: LIMPII/SR-B2 Antibody [NB400-129] - Lysotracker Red accumulates in endolysosomes.Immunofluorescence of young (A), adult (B), & aged (C) urothelium incubated with Lysotracker Red (red) & co-stained with antibodies to LIMP2 (green structures) & phalloidin (blue), which labels the cortical actin cytoskeleton. Large, Lysotracker Red-positive endolysosomes are marked with white arrows. Underlying intermediate (IC) & basal cells (BC) show non-specific cytoplasmic Lysotracker Red staining that did not appear in umbrella cells (UC). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29883476), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
LIMPII/SR-B2 Antibody

Immunocytochemistry/ Immunofluorescence: LIMPII/SR-B2 Antibody [NB400-129] -

Immunocytochemistry/ Immunofluorescence: LIMPII/SR-B2 Antibody [NB400-129] - Lysotracker Red accumulates in endolysosomes.Immunofluorescence of young (A), adult (B), & aged (C) urothelium incubated with Lysotracker Red (red) & co-stained with antibodies to LIMP2 (green structures) & phalloidin (blue), which labels the cortical actin cytoskeleton. Large, Lysotracker Red-positive endolysosomes are marked with white arrows. Underlying intermediate (IC) & basal cells (BC) show non-specific cytoplasmic Lysotracker Red staining that did not appear in umbrella cells (UC). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29883476), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
LIMPII/SR-B2 Antibody - BSA Free

Western Blot: LIMPII/SR-B2 Antibody - BSA Free [NB400-129] -

Loss of tomosyns does not affect levels of endo-lysosomal proteins.(A) Representative images of LAMP1 immunostaining in DIV14 neurons. Scale bar 20 um. (B) Quantification of the mean LAMP1 intensity in control and double knockout (DKO) neurons from confocal microscopy images as exemplified in (A). Data are shown as mean +/- SD and were analyzed using a two-tailed unpaired t-test. n=10 neurons/genotype. ns: not significant. (C) Levels of LAMP1 and LIMP2 as detected by western blot (WB) are normal in DKO neurons. Equal loading was verified by immunodetection of actin. (D) Quantification of LAMP1 and LIMP2 signal from WB images exemplified in (C). Levels of LAMP1 and LIMP2 in DKO were normalized to control levels in the corresponding culture. DKO data are presented as mean +/- SD and were analyzed using one sample t-test. n=9 samples/genotype from three culture preparations. ns: not significant.Figure 2—figure supplement 3—source data 1.Uncropped western blot (WB) images for Figure 2—figure supplement 2C.Uncropped western blot (WB) images for Figure 2—figure supplement 2C. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37695731), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for LIMPII/SR-B2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:250

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

reported in scientific literature (PMID 23863627)

Immunoprecipitation

1:100

Western Blot

1:1000
Application Notes
In Western Blot, a band is observed at ~85 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reviewed Applications

Read 1 review rated 4 using NB400-129 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: LIMPII/SR-B2

LIMP II (lysosome membrane protein 2; also called LGP85, SCARB2, CD36 antigen-like 2/CD36L2) is a member of the scavenger receptor class B member 2 family, ubiquitously expressed in higher organisms as a major lysosomal and endosomal membrane protein. LIMP II localize primarily to late endosomes/lysosomes as type III transmembrane protein, which traverses the membrane twice with the uncleaved signal peptide and a hydrophobic region near to the COOH-terminus, followed by twenty-amino acid long cytoplasmic tail of which a large portion located in lysosomal lumenal side possess 10-11 N-glycosylation sites. LIMP II acts as a lysosomal receptor for glucosylceramidase (GBA) targeting and it plays a key role in regulating the biogenesis of the endosomal/lysosomal compartments (ELCs). LIMP II serves as one of the major components of the molecular machinery required for intercompartmental membrane trafficking along the endocytic pathway rather than solely serving as a structural constituent of late endosomes and lysosomes. LIMP II deficiency has been found to result in the development of an obstruction of ureteric pelvic junction, deafness and peripheral neuropathy.

Long Name

Lysosomal Integral Membrane Protein II

Alternate Names

CD36L2, LPG85, SCARB2, SR-B2, SR-BII, SRB2

Entrez Gene IDs

950 (Human); 12492 (Mouse)

Gene Symbol

SCARB2

Additional LIMPII/SR-B2 Products

Product Documents for LIMPII/SR-B2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for LIMPII/SR-B2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Monocyte Markers

Citations for LIMPII/SR-B2 Antibody - BSA Free

Customer Reviews for LIMPII/SR-B2 Antibody - BSA Free (1)

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Customer Images

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  • LIMPII/lpg85 Antibody
    Name: Anonymous
    Application: Simple Western
    Sample Tested: SVG-A lysate and B103 lysate
    Species: Human and Rat
    Verified Customer | Posted 09/03/2018
    CHAPS-solublized SVG-A and B103 cell lyates, at the indicated concentrations, were probed with a 1:25 dil of antibody.
    LIMPII/SR-B2 Antibody - BSA Free NB400-129

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FAQs for LIMPII/SR-B2 Antibody - BSA Free

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  • Q: Can you please inform me about the peptide used as immunogen? It is referred that is the a C-terminal synthetic peptide made to the mouse LIMPII/lgp85 protein sequence, but the amino acid sequence used is not indicated.

    A: NB400-129 was made to a sequence between amino acids 425-480.

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