Lipin 1 Antibody (OTI8F10)

Novus Biologicals | Catalog # NBP2-45670

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI8F10
View all Lipin 1 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human LPIN1 (NP_663731) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

98.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Lipin 1 Antibody (OTI8F10)

Western Blot: Lipin 1 Antibody (OTI8F10) [NBP2-45670]

Western Blot: Lipin 1 Antibody (OTI8F10) [NBP2-45670]

Western Blot: Lipin 1 Antibody (8F10) [NBP2-45670] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Lipin 1.
Immunohistochemistry: Lipin 1 Antibody (OTI8F10) [NBP2-45670]

Immunohistochemistry: Lipin 1 Antibody (OTI8F10) [NBP2-45670]

Immunohistochemistry: Lipin 1 Antibody (8F10) [NBP2-45670] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Lipin 1 Antibody (OTI8F10) [NBP2-45670]

Immunohistochemistry: Lipin 1 Antibody (OTI8F10) [NBP2-45670]

Immunohistochemistry: Lipin 1 Antibody (8F10) [NBP2-45670] - Analysis of Adenocarcinoma of Human breast. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Lipin 1 Antibody (OTI8F10) [NBP2-45670]

Immunohistochemistry: Lipin 1 Antibody (OTI8F10) [NBP2-45670]

Immunohistochemistry: Lipin 1 Antibody (8F10) [NBP2-45670] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Lipin 1 Antibody (OTI8F10) [NBP2-45670]

Immunohistochemistry: Lipin 1 Antibody (OTI8F10) [NBP2-45670]

Immunohistochemistry: Lipin 1 Antibody (8F10) [NBP2-45670] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Lipin 1 Antibody (OTI8F10) [NBP2-45670]

Immunohistochemistry: Lipin 1 Antibody (OTI8F10) [NBP2-45670]

Immunohistochemistry: Lipin 1 Antibody (8F10) [NBP2-45670] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Lipin 1 Antibody (OTI8F10) [NBP2-45670]

Immunohistochemistry: Lipin 1 Antibody (OTI8F10) [NBP2-45670]

Immunohistochemistry: Lipin 1 Antibody (8F10) [NBP2-45670] - Analysis of Carcinoma of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Lipin 1 Antibody (OTI8F10) [NBP2-45670]

Immunohistochemistry: Lipin 1 Antibody (OTI8F10) [NBP2-45670]

Immunohistochemistry: Lipin 1 Antibody (8F10) [NBP2-45670] - Analysis of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Lipin 1 Antibody (OTI8F10) [NBP2-45670]

Immunohistochemistry: Lipin 1 Antibody (OTI8F10) [NBP2-45670]

Immunohistochemistry: Lipin 1 Antibody (8F10) [NBP2-45670] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Lipin 1 Antibody (OTI8F10) [NBP2-45670]

Immunohistochemistry: Lipin 1 Antibody (OTI8F10) [NBP2-45670]

Immunohistochemistry: Lipin 1 Antibody (8F10) [NBP2-45670] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Lipin 1 Antibody (OTI8F10) [NBP2-45670]

Immunohistochemistry: Lipin 1 Antibody (OTI8F10) [NBP2-45670]

Immunohistochemistry: Lipin 1 Antibody (8F10) [NBP2-45670] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Lipin 1 Antibody (OTI8F10) [NBP2-45670]

Immunohistochemistry: Lipin 1 Antibody (OTI8F10) [NBP2-45670]

Immunohistochemistry: Lipin 1 Antibody (8F10) [NBP2-45670] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Lipin 1 Antibody (OTI8F10) [NBP2-45670]

Immunohistochemistry: Lipin 1 Antibody (OTI8F10) [NBP2-45670]

Immunohistochemistry: Lipin 1 Antibody (8F10) [NBP2-45670] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Lipin 1 Antibody (OTI8F10) [NBP2-45670]

Immunohistochemistry: Lipin 1 Antibody (OTI8F10) [NBP2-45670]

Immunohistochemistry: Lipin 1 Antibody (8F10) [NBP2-45670] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Lipin 1 Antibody (OTI8F10) [NBP2-45670]

Immunohistochemistry: Lipin 1 Antibody (OTI8F10) [NBP2-45670]

Immunohistochemistry: Lipin 1 Antibody (8F10) [NBP2-45670] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Lipin 1 Antibody (OTI8F10) [NBP2-45670]

Immunohistochemistry: Lipin 1 Antibody (OTI8F10) [NBP2-45670]

Immunohistochemistry: Lipin 1 Antibody (8F10) [NBP2-45670] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Lipin 1 Antibody (OTI8F10) [NBP2-45670]

Immunohistochemistry: Lipin 1 Antibody (OTI8F10) [NBP2-45670]

Immunohistochemistry: Lipin 1 Antibody (8F10) [NBP2-45670] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Lipin 1 Antibody (OTI8F10) [NBP2-45670]

Immunohistochemistry: Lipin 1 Antibody (OTI8F10) [NBP2-45670]

Immunohistochemistry: Lipin 1 Antibody (8F10) [NBP2-45670] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Lipin 1 Antibody (OTI8F10) [NBP2-45670]

Immunohistochemistry: Lipin 1 Antibody (OTI8F10) [NBP2-45670]

Immunohistochemistry: Lipin 1 Antibody (8F10) [NBP2-45670] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Lipin 1 Antibody (OTI8F10) [NBP2-45670]

Immunohistochemistry: Lipin 1 Antibody (OTI8F10) [NBP2-45670]

Immunohistochemistry: Lipin 1 Antibody (8F10) [NBP2-45670] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Applications for Lipin 1 Antibody (OTI8F10)

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Lipin 1

LPIN1 (Lipin-1) is crucial for normal adipose tissue development and metabolism. LPIN1 selectively activates a subset of PGC-225; target pathways, including fatty acid oxidation and mitochondrial oxidative phosphorylation by inducing expression of the nuclear receptor PPAR 225. LPIN1 also inactivates the lipogenic program and suppresses circulating lipid levels. An abundance of LPIN1 promotes fat accumulation and insulin sensitivity. A deficiency in LPIN1 may deter normal adipose tissue development, resulting in insulin resistance and lipodystrophy, a heterogeneous group of disorders characterized by loss of body fat, fatty liver, hypertriglyceridemia and insulin resistance.

Long Name

Phosphatidate Phosphatase LPIN1

Alternate Names

LPIN1

Gene Symbol

LPIN1

Additional Lipin 1 Products

Product Documents for Lipin 1 Antibody (OTI8F10)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Lipin 1 Antibody (OTI8F10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Obesity
Phosphatases and Regulators

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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Associated Pathways

mTOR Signaling Pathway mTOR Signaling Pathway Thumbnail