Lipolysis Stimulated Lipoprotein Receptor Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-89631
![Western Blot: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631]](https://resources.rndsystems.com/images/products/Lipolysis-Stimulated-Lipoprotein-Receptor-Antibody-Western-Blot-NBP1-89631-img0026.jpg "Western Blot: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631]")
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Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western
Cited:
Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: MSEVTSLHEDDWRSRPSRGPALTPIRDEEWGGHSPRSPRGWDQEPAREQAGGGWRARRPRARSVDALDDLTPPSTAESGSRSPTSNGGRSRAYMPPRSRSRDDLYDQDDS
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Lipolysis Stimulated Lipoprotein Receptor Antibody - BSA Free
Western Blot: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631]
Western Blot: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Analysis in human cell lines A-431 and U-251MG using anti-LSR antibody. Corresponding LSR RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.![Immunocytochemistry/ Immunofluorescence: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631]](https://resources.rndsystems.com/images/products/Lipolysis-Stimulated-Lipoprotein-Receptor-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89631-img0015.jpg "Immunocytochemistry/ Immunofluorescence: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631]")
Immunocytochemistry/ Immunofluorescence: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631]
Immunocytochemistry/Immunofluorescence: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & cytosol. Antibody staining is shown in green.![Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631]](https://resources.rndsystems.com/images/products/Lipolysis-Stimulated-Lipoprotein-Receptor-Antibody-Immunohistochemistry-Paraffin-NBP1-89631-img0020.jpg "Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631]")
Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631]
Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Staining of human liver shows moderate membranous positivity in hepatocytes.![Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631]](https://resources.rndsystems.com/images/products/Lipolysis-Stimulated-Lipoprotein-Receptor-Antibody-Immunohistochemistry-Paraffin-NBP1-89631-img0022.jpg "Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631]")
Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631]
Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Staining of human stomach shows strong membranous positivity in glandular cells.![Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631]](https://resources.rndsystems.com/images/products/Lipolysis-Stimulated-Lipoprotein-Receptor-Antibody-Immunohistochemistry-Paraffin-NBP1-89631-img0023.jpg "Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631]")
Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631]
Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Staining of human testis shows moderate membranous and cytoplasmic positivity in cells in seminiferous ducts.![Lipolysis Stimulated Lipoprotein Receptor Antibody - BSA Free Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody - BSA Free [NBP1-89631]](https://resources.rndsystems.com/images/products/nbp1-89631_rabbit-polyclonal-lipolysis-stimulated-lipoprotein-receptor-antibody-135202517442159.jpg "Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody - BSA Free [NBP1-89631]")
Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody - BSA Free [NBP1-89631]
Staining of human stomach shows strong membranous positivity in glandular cells.![Lipolysis Stimulated Lipoprotein Receptor Antibody - BSA Free Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody - BSA Free [NBP1-89631]](https://resources.rndsystems.com/images/products/nbp1-89631_rabbit-polyclonal-lipolysis-stimulated-lipoprotein-receptor-antibody-15520258303312.jpg "Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody - BSA Free [NBP1-89631]")
Immunohistochemistry-Paraffin: Lipolysis Stimulated Lipoprotein Receptor Antibody - BSA Free [NBP1-89631]
Staining of human liver shows moderate membranous positivity in hepatocytes.![Simple Western: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631]](https://resources.rndsystems.com/images/products/Lipolysis-Stimulated-Lipoprotein-Receptor-Antibody-Simple-Western-NBP1-89631-img0006.jpg "Simple Western: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631]")
Simple Western: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631]
Simple Western: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Simple Western lane view shows a specific band for LSR in 0.2 mg/ml of RT-4 (left), A431 (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.![Simple Western: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631]](https://resources.rndsystems.com/images/products/Lipolysis-Stimulated-Lipoprotein-Receptor-Antibody-Simple-Western-NBP1-89631-img0008.jpg "Simple Western: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631]")
Simple Western: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631]
Simple Western: Lipolysis Stimulated Lipoprotein Receptor Antibody [NBP1-89631] - Electropherogram image(s) of corresponding Simple Western lane view. Lipolysis Stimulated Lipoprotein Receptor antibody was used at 1:20 dilution on RT-4 and A431 lysate(s).Applications for Lipolysis Stimulated Lipoprotein Receptor Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Simple Western
1:20
Western Blot
0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
See Simple Western Antibody Database for Simple Western validation: Tested in RT-4, A431, separated by Size, antibody dilution of 1:20, apparent MW was 70 kDa
See Simple Western Antibody Database for Simple Western validation: Tested in RT-4, A431, separated by Size, antibody dilution of 1:20, apparent MW was 70 kDa
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: LSR
Long Name
Lipolysis Stimulated Lipoprotein Receptor
Alternate Names
ILDR3, LISCH, LISCH7
Gene Symbol
LSR
Additional LSR Products
Product Documents for Lipolysis Stimulated Lipoprotein Receptor Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Lipolysis Stimulated Lipoprotein Receptor Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for Lipolysis Stimulated Lipoprotein Receptor Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Lipolysis Stimulated Lipoprotein Receptor Antibody - BSA Free
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Q: In the datasheet of your antibody against the Lipolysis-stimulated lipoprotein receptor (NBP1-89631) you show a figure in which a immunohistochemistry staining is shown. Can you please give an explanation why the cytoplasm of the cells is stained? Because
A: Lipolysis Stimulated Lipoprotein Receptor/LSR is a 649 amino acids long (71.4 kDa) trans-membrane protein with Extracellular (aa 1-259), Helical (260-280) and Cytoplasmic (281 – 649) domains Uniprot Site. Please note that NBP1-89631 was generated against a peptide corresponding to aa 431-540 of human LSR, and this sequence falls within cytoplasmic domain of LSR protein. Accordingly, NBP1-89631 is expected to bind the cytoplasmic domain of LSR protein and in immunostaining assays, the expected staining pattern with this antibody would be cytoplasmic as shown in the validation image of its datasheet.
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