LMAN1 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-04910

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all LMAN1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 270-480 of human LMAN1 (NP_005561.1). PGKEPPTPDKEISEKEKEKYQEEFEHFQQELDKKKEEFQKGHPDLQGQPAEEIFESVGDRELRQVFEGQNRIHLEIKQLNRQLDMILDEQRRYVSSLTEEISKRGAGMPGQHGQITQQELDTVVKTQHEILRQVNEMKNSMSETVRLVSGMQHPGSAGGVYETTQHFIDIKEHLHIVKRDIDNLVQRNMPSNEKPKCPELPPFPSCLSTVH

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LMAN1 Antibody - Azide and BSA Free

Western Blot: LMAN1 AntibodyAzide and BSA Free [NBP3-04910]

Western Blot: LMAN1 AntibodyAzide and BSA Free [NBP3-04910]

Western Blot: LMAN1 Antibody [NBP3-04910] - Analysis of extracts of various cell lines, using LMAN1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit
LMAN1 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: LMAN1 Antibody - Azide and BSA Free [NBP3-04910] -

Immunocytochemistry/ Immunofluorescence: LMAN1 Antibody - Azide and BSA Free [NBP3-04910] - Immunofluorescence analysis of C6 cells using LMAN1 Rabbit pAb (A10440) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
LMAN1 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: LMAN1 Antibody - Azide and BSA Free [NBP3-04910] -

Immunocytochemistry/ Immunofluorescence: LMAN1 Antibody - Azide and BSA Free [NBP3-04910] - Immunofluorescence analysis of U-2 OS cells using LMAN1 Rabbit pAb (A10440) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
LMAN1 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: LMAN1 Antibody - Azide and BSA Free [NBP3-04910] -

Immunocytochemistry/ Immunofluorescence: LMAN1 Antibody - Azide and BSA Free [NBP3-04910] - Immunofluorescence analysis of NIH-3T3 cells using LMAN1 Rabbit pAb (A10440) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for LMAN1 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:100

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LMAN1

Protein trafficking along the endoplasmic reticulum (ER) to the Golgi apparatus occurs by vesicular transport between membranous compartments. LMAN1 is a mannose-specific lectin transport receptor protein which cycles between the ER and the Golgi complex. LMAN1 is involved in intracellular transport of many glycoproteins. LMAN1 is useful in studying mechanisms underlying protein trafficking early in the secretory pathway.

Alternate Names

ERGIC-53, ERGIC53ER-Golgi intermediate compartment 53 kDa protein, F5F8Dendoplasmic reticulum-golgi intermediate compartment protein 53, FMFD1, Gp58, intracellular mannose specific lectin, Intracellular mannose-specific lectin MR60, Lectin mannose-binding 1, lectin, mannose-binding, 1, MCFD1coagulation factor V-factor VIII combined deficiency, MR60, protein ERGIC-53

Gene Symbol

LMAN1

Additional LMAN1 Products

Product Documents for LMAN1 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for LMAN1 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Protocols

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