LRPAP Antibody (009) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-90513
Recombinant Monoclonal Antibody
![Western Blot: LRPAP Antibody (009) [NBP2-90513]](https://beta-resources.rndsystems.com/images/products/LRPAP-Antibody-009-Western-Blot-NBP2-90513-img0003.jpg "Western Blot: LRPAP Antibody (009) [NBP2-90513]")
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 009 expressed in HEK293
Format
Azide and BSA Free
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Product Specifications
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse LRPAP (Accession#: NP_038615.2; Gln 29-Leu 360).
Specificity
No cross-reactivity in ELISA with: Human LRPAP1
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for LRPAP Antibody (009) - Azide and BSA Free
Western Blot: LRPAP Antibody (009) [NBP2-90513]
Western Blot: LRPAP Antibody (009) [NBP2-90513] - Anti-LRPAP rabbit monoclonal antibody at 1:500 dilution. Lane A: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 41 kDa. Observed band size: 41 kDa![Immunohistochemistry-Paraffin: LRPAP Antibody (009) [NBP2-90513]](https://beta-resources.rndsystems.com/images/products/LRPAP-Antibody-009-Immunohistochemistry-Paraffin-NBP2-90513-img0005.jpg "Immunohistochemistry-Paraffin: LRPAP Antibody (009) [NBP2-90513]")
Immunohistochemistry-Paraffin: LRPAP Antibody (009) [NBP2-90513]
Immunohistochemistry-Paraffin: LRPAP Antibody (009) [NBP2-90513] - Immunochemical staining of mouse LRPAP in mouse brain with rabbit monoclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections. The image showing staining of nerve cells.![Immunohistochemistry-Paraffin: LRPAP Antibody (009) [NBP2-90513]](https://beta-resources.rndsystems.com/images/products/LRPAP-Antibody-009-Immunohistochemistry-Paraffin-NBP2-90513-img0004.jpg "Immunohistochemistry-Paraffin: LRPAP Antibody (009) [NBP2-90513]")
Immunohistochemistry-Paraffin: LRPAP Antibody (009) [NBP2-90513]
Immunohistochemistry-Paraffin: LRPAP Antibody (009) [NBP2-90513] - Immunochemical staining of mouse LRPAP in mouse brain with rabbit monoclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections. The image showing staining of nephric tubule.![Immunoprecipitation: LRPAP Antibody (009) [NBP2-90513]](https://beta-resources.rndsystems.com/images/products/LRPAP-Antibody-009-Immunoprecipitation-NBP2-90513-img0006.jpg "Immunoprecipitation: LRPAP Antibody (009) [NBP2-90513]")
Immunoprecipitation: LRPAP Antibody (009) [NBP2-90513]
Immunoprecipitation: LRPAP Antibody (009) [NBP2-90513] - Mouse LRPAP was immunoprecipitated using: Lane A: 0.5 mg A549 Whole Cell Lysate0.5 ul anti-Mouse LRPAP rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse LRPAP rabbit monoclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 41 kDa. Observed band size: 41 kDa
Immunocytochemistry/Immunofluorescence: LRPAP Antibody (009) [NBP2-90513] -
Immunocytochemistry/Immunofluorescence: LRPAP Antibody (009) [NBP2-90513] - Immunofluorescence staining of mouse LRPAP in NIH-3T3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-90513 antibody (dilution ratio 1:60) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Endoplasmic reticulum.Applications for LRPAP Antibody (009) - Azide and BSA Free
Application
Recommended Usage
ELISA
1:25000-1:50000
Immunocytochemistry/ Immunofluorescence
1:20-1:100
Immunohistochemistry-Paraffin
1:500-1:2000
Immunoprecipitation
0.2-1 uL/mg of lysate
Western Blot
1:500-1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: LRPAP
Long Name
Low Density Lipoprotein-Related Protein-associated Protein
Alternate Names
A2MRAP, A2RAP, HBP44, LRPAP1, RAP
Gene Symbol
LRPAP1
Additional LRPAP Products
Product Documents for LRPAP Antibody (009) - Azide and BSA Free
Product Specific Notices for LRPAP Antibody (009) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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