LRPAP Antibody (7F1) - Chimeric - Azide and BSA Free

Name: LRPAP Antibody (7F1) - Chimeric - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP3-12047
Price: 209 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-12047

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # 7F1

Format

Azide and BSA Free

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Product Specifications

Immunogen

This antibody was raised by immunising mice with human placental LRPAP.

Specificity

This antibody is specific for human LRPAP. It does not cross-react with rabbit or murine LRPAP.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for LRPAP Antibody (7F1) - Chimeric - Azide and BSA Free

Western Blot: LRPAP Antibody (7F1)ChimericAzide and BSA Free [NBP3-12047]

Western Blot: LRPAP Antibody (7F1) - Chimeric [NBP3-12047] - Staining in HeLa(A) (0.1ug/ml), A549 (B) (0.01ug/ml), HepG2 (C) (0.01ug/ml), HEK293 (D) (0.03ug/ml), MCF7 (E) (0.03ug/ml), Caco-2 (F) (0.001ug/ml) and U251 (G) (0.001ug/ml) cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of RAP (NBP3-12047) at 0.5 ug/ml, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Immunocytochemistry/ Immunofluorescence: LRPAP Antibody (7F1) - Chimeric - Azide and BSA Free [NBP3-12047]

Immunocytochemistry/Immunofluorescence: LRPAP Antibody (7F1) - Chimeric [NBP3-12047] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 7F1 (NBP3-12047) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12047, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.

Flow Cytometry: LRPAP Antibody (7F1) - Chimeric - Azide and BSA Free [NBP3-12047] -

Flow Cytometry: LRPAP Antibody (7F1) - Chimeric - Azide and BSA Free [NBP3-12047] - HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12047 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.