Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat, Primate

Cited:

Human, Mouse, Primate

Predicted:

Porcine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow (Cell Surface), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all LRRK2 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to a C-terminal region, within residues 2500-2527 of the human LRRK2 protein, conjugated to KLH. [Swiss-Prot# Q5S007]

Localization

Cytoplasm. Membrane; Peripheral membrane protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

286 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LRRK2 Antibody - BSA Free

Knockout Validated: LRRK2 Antibody [NB110-58771]

Western Blot: LRRK2 Antibody [NB110-58771]

Western Blot: LRRK2 Antibody [NB110-58771] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and LRRK2 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human LRRK2 Polyclonal Antibody (Catalog # NB110-58771) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for LRRK2 at approximately 275 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Western Blot: LRRK2 Antibody [NB110-58771]

Western Blot: LRRK2 Antibody [NB110-58771]

Western Blot: LRRK2 Antibody [NB110-58771] - NB110-58771 in increasing amounts of human lymphoblast
Immunocytochemistry/ Immunofluorescence: LRRK2 Antibody [NB110-58771]

Immunocytochemistry/ Immunofluorescence: LRRK2 Antibody [NB110-58771]

Immunocytochemistry/Immunofluorescence: LRRK2 Antibody [NB110-58771] - Immunofluorescence of Lrrk2 with NB110-58771 within (A) Substantia nigra neurons (B) Chains of migrating neuroblasts.
Immunohistochemistry-Frozen: LRRK2 Antibody [NB110-58771]

Immunohistochemistry-Frozen: LRRK2 Antibody [NB110-58771]

Immunohistochemistry-Frozen: LRRK2 Antibody [NB110-58771] - DAB Immunostaining in frozen sections of mouse brainstem.
Knockdown Validated: LRRK2 Antibody [NB110-58771]

Western Blot: LRRK2 Antibody [NB110-58771]

LRRK2-Antibody-Knockdown-Validated-NB110-58771-img0010.jpg
LRRK2 Antibody

Western Blot: LRRK2 Antibody [NB110-58771] -

Western Blot: LRRK2 Antibody [NB110-58771] - Characterization of iPSC-derived DA neurons with LRRK2 mutations. a Diagram showing the DA differentiation protocol used for neural induction of human iPSC lines. b Temporal gene expression analyzed by qRT-PCR at three time points: induction (3 weeks), expansion (4–5 weeks), & maturation (>6 weeks). Each point represents the mean ± SEM of at least two independent differentiation experiments. c Representative images of mature neuronal cultures showing expression of neuronal ( beta III-tubulin, Tau, & alpha -synuclein) & dopaminergic (TH, NURR1) markers. Nuclei were counterstained with Hoechst. Scale bars: 50 μm. d Quantification of immunostainings. Data are represented as mean ± SEM of counts from at least two different lines for each genotype. e Representative western blot analyses of TH, Tau, & GFAP with beta III-tubulin as loading control in iPSC-derived mature neurons. f Representative immunoblots & quantification of LRRK2 expression in mature neuronal cultures. alpha -tubulin was the loading control & data were normalized to control WT neurons. Bars represent the mean ± SEM of at least two different lines per genotype. DIV days in vitro, GEL gelatin, POL poly-ornithine, FBN fibronectin, LMN laminin, N2 N2 supplement, bFGF basic fibroblast growth factor, SAG smoothened agonist, LDN LDN-193189, CHIR CHIR99021, SB SB431542, BDNF brain-derived neurotrophic factor, AA ascorbic acid, B27 B27 supplement, dbcAMP dibutyryl cyclic adenosine monophosphate, TGF beta III transforming growth factor beta III, GDNF glial derived neurotrophic factor. See Additional file 2 for uncropped blots Image collected & cropped by CiteAb from the following publication (http://jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-016…), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for LRRK2 Antibody - BSA Free

Application
Recommended Usage

Flow (Cell Surface)

reported in scientific literature (PMID 20483355)

Immunocytochemistry/ Immunofluorescence

1:500-1:1000

Immunohistochemistry

1:200-1:500

Immunohistochemistry-Paraffin

reported in scientific literature (PMID 24312256)

Immunoprecipitation

1:10-1:500

Western Blot

1:1000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: LRRK2

LRRK2 (leucine-rich repeat serine/threonine-protein kinase 2, also called PARK8 or Dardarin) is a homodimeric multifunctional protein that belongs to TKL serine threonine protein kinases family and LRRK2 immunoreactivity has been documented in neurofibrillary tangles in Alzheimer's disease as well as in PDCG (parkinsonism-dementia complex of Guam). Highly expressed in tissues such as liver, heart, brain etc., LRRK2 localizes to cellular cytoplasm and also associates with membrane structures including mitochondrial outer membrane. LRRK interacts with PARK2, PRDX3 and TPCN2, and phosphorylate proteins which are implicated in Parkinson disease. LRRK2 is a cytoplasmic kinase with autophosphorylation capability as well as GTPase activity. Its interaction with microtubules suggests that LRRK2-induced neurodegeneration might be partly mediated via inhibition of microtubule dynamics, and LRRK2 -mitochondria interactiosn suggests its implication in pathways eliciting ROS mediated damage. LRRK2 has been shown exert positive regulation in autophagy via calcium-sensitive activation of CaMKK/AMPK signaling pathway through activation of NAADP receptors, increase of lysosomal pH as well as calcium release.

Long Name

Leucine-rich Repeat Ser/Thr Protein Kinase 2

Alternate Names

AURA17, Dardarin, PARK8, ROCO2

Gene Symbol

LRRK2

Additional LRRK2 Products

Product Documents for LRRK2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for LRRK2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for LRRK2 Antibody - BSA Free

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Protocols

View specific protocols for LRRK2 Antibody - BSA Free (NB110-58771):

LRRK2 Antibody:
Immunostaining of frozen sections using streptavidin or Exvtravidin peroxidase and DAB

1. Make 10 micron frozen sections.
2. Fix tissue sections on slides in cold acetone, 10% buffered folrmalin or 4% paraformaldehyde for 8 to 10 min.
3. Rinse slides with PBS by immersion for 2 min. Repeat.
4. Block all slides with 5% milk in PBST for 30 min. at RT.
5. Remove blocking solution and add NB 110-58771 LRRK2 primary antibody 1:100 to 1:200 in PBS with 0.1% triton X-100 with 5% milk.
6. Incubate slides at 4degrees Celcius, overnight.
7. Rinse slides with PBST by immersion for 2 min. Repeat.
8. Add biotinylated donkey anti-rabbit secondary antibody (1:500 in PBST with 5% milk). Incubate 1 hour at RT.
9. Rinse with PBST by immersion 2 min. Repeat.
10. Add streptavidin-HRP or Extravidin-HRP. Incubate at RT for 1 hr.
11. Rinse with PBS by immersion for 1 min. Repeat.
12. Make DAB solution.
13. Add DAB to slides. Incubate 5-7 minutes.
14. Stop DAB reaction by immersion in water.
15. Counterstain sections, if desired.
16. Dehydrate,defat and coverslip slides.

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FAQs for LRRK2 Antibody - BSA Free

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  • Q: Is the immunogen of NB110-58771 within amino acids 922 and 1143 of LRRK2 (Uniprot ID#Q5S007)?

    A: The immunogen used to generate NB110-58771 was a synthetic peptide made to a C-terminal region within residues 2500-2527 of the human LRRK2 protein (UniProt # Q5S007). We do have an alternative antibody that you may be interested in, with catalog number NB110-55289. The immunogen used to generate this product was synthetic peptide made to an internal region of the mouse LRRK2 protein sequence (between residues 900-1000), although this does not recognize human LRRK2. If you are looking to detect mouse LRRK2 this would be a suitable product for you.

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