Lunapark Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-80637
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Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: GALDRIVEYLVGDGPQNRYALICQQCFSHNGMALKEEFEYIAFRCAYCFFLNPARKTRPQAPRLPEFSFEKRQVVEGSSSVGPLPSGSVLSSDNQFNEESLEHDVLDDNTEQTDDKIPATEQ
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Lunapark Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: Lunapark Antibody [NBP1-80637]
Immunocytochemistry/Immunofluorescence: Lunapark Antibody [NBP1-80637] - Staining of human cell line U-251 MG shows localization to nucleoplasm & endoplasmic reticulum. Antibody staining is shown in green.Immunohistochemistry-Paraffin: Lunapark Antibody [NBP1-80637]
Immunohistochemistry-Paraffin: Lunapark Antibody [NBP1-80637] - Staining of human tonsil shows strong granular cytoplasmic positivity in germinal center cells.Western Blot: Lunapark Antibody [NBP1-80637]
Western Blot: Lunapark Antibody [NBP1-80637] - Analysis in human cell line U-138 MG.Immunohistochemistry-Paraffin: Lunapark Antibody [NBP1-80637]
Immunohistochemistry-Paraffin: Lunapark Antibody [NBP1-80637] - Staining of human cerebellum shows strong granular cytoplasmic positivity in Purkinje cells.Immunohistochemistry-Paraffin: Lunapark Antibody [NBP1-80637]
Immunohistochemistry-Paraffin: Lunapark Antibody [NBP1-80637] - Staining of human cervix shows moderate granular cytoplasmic positivity in squamous epithelial cells.Immunohistochemistry-Paraffin: Lunapark Antibody [NBP1-80637]
Immunohistochemistry-Paraffin: Lunapark Antibody [NBP1-80637] - Staining of human testis shows strong granular cytoplasmic positivity in cells in seminiferous ducts.Western Blot: Lunapark Antibody - BSA Free [NBP1-80637] -
Maternal obesity compromises glucose and ER homeostasis concomitantly through miR-126-3p programming in eWAT of adult offspring. (a) Normalised relative miR-126-3p, Irs1 and Lnpk expression (n = 5 per group). (b) Representative blots followed by normalised (c) IRS-1 and (d) Lunapark protein expression, n = 6 for both. (e) Altered relative expression of ER-related genes, n = 5–6 per group. (f) Percentage of starting glucose during glucose tolerance test, n = 6–7 per group. Data in (a, c–e) were analysed by unpaired Student’s t test (CC vs OC offspring). Data in (f) were analysed by two-way (repeated measures) ANOVA followed by Bonferroni’s multiple comparisons test; *p < 0.05, **p < 0.01 and ***p < 0.001. All the graphs represent mean +/- SEM Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33501603), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for Lunapark Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Lunapark
Alternate Names
2310011O18Rik, 4921514L11Rik, DKFZp686G1442, KIAA1715, LNP, Lunapark, MGC102862, protein lunapark, Ul, ulnaless
Entrez Gene IDs
80856 (Human)
Gene Symbol
LNPK
Additional Lunapark Products
Product Documents for Lunapark Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Lunapark Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for Lunapark Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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