Lunapark Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-80637

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GALDRIVEYLVGDGPQNRYALICQQCFSHNGMALKEEFEYIAFRCAYCFFLNPARKTRPQAPRLPEFSFEKRQVVEGSSSVGPLPSGSVLSSDNQFNEESLEHDVLDDNTEQTDDKIPATEQ

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Lunapark Antibody - BSA Free

Western Blot: Lunapark Antibody [NBP1-80637]

Western Blot: Lunapark Antibody [NBP1-80637]

Western Blot: Lunapark Antibody [NBP1-80637] - Analysis in U-138MG cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: Lunapark Antibody [NBP1-80637]

Immunocytochemistry/ Immunofluorescence: Lunapark Antibody [NBP1-80637]

Immunocytochemistry/Immunofluorescence: Lunapark Antibody [NBP1-80637] - Staining of human cell line U-251 MG shows localization to nucleoplasm & endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Lunapark Antibody [NBP1-80637]

Immunohistochemistry-Paraffin: Lunapark Antibody [NBP1-80637]

Immunohistochemistry-Paraffin: Lunapark Antibody [NBP1-80637] - Staining of human tonsil shows strong granular cytoplasmic positivity in germinal center cells.
Western Blot: Lunapark Antibody [NBP1-80637]

Western Blot: Lunapark Antibody [NBP1-80637]

Western Blot: Lunapark Antibody [NBP1-80637] - Analysis in human cell line U-138 MG.
Immunohistochemistry-Paraffin: Lunapark Antibody [NBP1-80637]

Immunohistochemistry-Paraffin: Lunapark Antibody [NBP1-80637]

Immunohistochemistry-Paraffin: Lunapark Antibody [NBP1-80637] - Staining of human cerebellum shows strong granular cytoplasmic positivity in Purkinje cells.
Immunohistochemistry-Paraffin: Lunapark Antibody [NBP1-80637]

Immunohistochemistry-Paraffin: Lunapark Antibody [NBP1-80637]

Immunohistochemistry-Paraffin: Lunapark Antibody [NBP1-80637] - Staining of human cervix shows moderate granular cytoplasmic positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: Lunapark Antibody [NBP1-80637]

Immunohistochemistry-Paraffin: Lunapark Antibody [NBP1-80637]

Immunohistochemistry-Paraffin: Lunapark Antibody [NBP1-80637] - Staining of human testis shows strong granular cytoplasmic positivity in cells in seminiferous ducts.
Lunapark Antibody - BSA Free

Western Blot: Lunapark Antibody - BSA Free [NBP1-80637] -

Maternal obesity compromises glucose and ER homeostasis concomitantly through miR-126-3p programming in eWAT of adult offspring. (a) Normalised relative miR-126-3p, Irs1 and Lnpk expression (n = 5 per group). (b) Representative blots followed by normalised (c) IRS-1 and (d) Lunapark protein expression, n = 6 for both. (e) Altered relative expression of ER-related genes, n = 5–6 per group. (f) Percentage of starting glucose during glucose tolerance test, n = 6–7 per group. Data in (a, c–e) were analysed by unpaired Student’s t test (CC vs OC offspring). Data in (f) were analysed by two-way (repeated measures) ANOVA followed by Bonferroni’s multiple comparisons test; *p < 0.05, **p < 0.01 and ***p < 0.001. All the graphs represent mean +/- SEM Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33501603), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Lunapark Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lunapark

KIAA1715 may be involved in limb and central nervous system development

Alternate Names

2310011O18Rik, 4921514L11Rik, DKFZp686G1442, KIAA1715, LNP, Lunapark, MGC102862, protein lunapark, Ul, ulnaless

Entrez Gene IDs

80856 (Human)

Gene Symbol

LNPK

Additional Lunapark Products

Product Documents for Lunapark Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Lunapark Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Lunapark Antibody - BSA Free

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Protocols

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FAQs

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