LXR alpha/NR1H3 Antibody

Western Blot: LXR alpha/NR1H3 Antibody [NB300-612]

Western Blot: LXR alpha/NR1H3 Antibody [NB300-612] - analysis was performed on nuclear enriched extracts (30 ug lysate) of MCF7 (Lane 1), THP-1 (Lane 2), A549 (Lane 3), SH-SY5Y (Lane 4), HL-60 (Lane 5) and KARPAS 299 (Lane 6). The blot was probed with Rabbit Anti-LXR alpha Polyclonal Antibody (2ug/ml) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (0.25 ug/ml, 1:4000 dilution). A 50 kDa band corresponding to LXR alpha was observed across the cell lines tested. Known quantity of protein samples was electrophoresed using 4-12 % Bis-Tris gel, Electrophoresis System and Sharp Pre-Stained Protein Standard. Resolved proteins were then transferred onto a nitrocellulose membrane with Blot 2 Dry Blotting System. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using ECL Chemiluminescent Substrate Reagent Kit.

Immunocytochemistry/ Immunofluorescence: LXR alpha/NR1H3 Antibody [NB300-612]

Immunocytochemistry/Immunofluorescence: LXR alpha/NR1H3 Antibody [NB300-612] - Analysis of LXR alpha (green) showing staining in the nucleus of MCF-7 cells (right) compared to a negative control without primary antibody (left).

Western Blot: LXR alpha/NR1H3 Antibody [NB300-612] -

LXRs are expressed in peripheral blood cells. (A) RNA from peripheral blood mononuclear cells obtained from normal human donors was assayed for LXR alpha and LXR beta transcript levels using qPCR. Expression values were normalized to GAPDH levels, represented as the mean +/- SEM. (B) LXR alpha protein levels in protein extracts from PBMCs from these same donors were detected by Western blotting using rabbit anti-human LXR alpha polyclonal antisera.