Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-06640

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Lysine (K)-specific Demethylase 6B/KDM6B Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to an internal portion of the mouse JMJD3 protein (within residues 600-650). [Swiss-Prot# Q5NCY0]

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody - BSA Free

Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 AntibodyBSA Free [NBP1-06640]

Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 AntibodyBSA Free [NBP1-06640]

Lysine-K-specific-Demethylase-6B-KDM6B-JMJD3-Antibody-Western-Blot-NBP1-06640-img0007.jpg
Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody - BSA Free [NBP1-06640]

Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody - BSA Free [NBP1-06640]

Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP1-06640] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-JMJD3 [NBP1-06640] at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 AntibodyBSA Free [NBP1-06640]

Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 AntibodyBSA Free [NBP1-06640]

Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP1-06640] - Analysis of JMJD3 expression on HepG2 whole cell lysates.
Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 AntibodyBSA Free [NBP1-06640]

Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 AntibodyBSA Free [NBP1-06640]

Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP1-06640] - Total protein from human HepG2 and mouse 3T3 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-JMJD3 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody - BSA Free

Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody - BSA Free [NBP1-06640] -

Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody - BSA Free [NBP1-06640] - KDM6A protein levels are decreased following EMT & upregulated during MET(A) HMLE cells were transduced with either an empty vector (HMLE-Vector), or genes encoding the EMT-inducing transcription factors Snail (HMLE-Snail) or Twist (HMLE-Twist) [4]. Whole-cell lysates were analyzed by immunoblotting for KDM6A. beta -actin was used as a loading control. (B-F) MCF10A cells were treated with sterile 4 mM HCl containing 0.1% bovine serum albumin (Vehicle) or TGFB for 5 days (TGFB+). In parallel, MCF10A cells were exposed to TGFB for 5 days in order to induce EMT, & subsequently subjected to TGFB-withdrawal, for a further 5 days, to elicit MET (TGFB+/-). (B-C) MCF10A cells, treated as described, were fixed & immunostained for E-cadherin (B) & N-cadherin (C). Nuclei were counterstained with DAPI (blue). Scale bars, 50 μm. (D) MCF10A cells, treated as described, were analyzed by immunoblotting for KDM6B & vimentin (Vim). COX IV was used as a loading control. (E) MCF10A cells, treated as described, were fixed & immunostained for KDM6A. Nuclei were counterstained with DAPI (blue). Scale bars, 50 μm. (F) Cells were analyzed by immunoblotting for KDM6A, EZH2 & RING1A. beta -actin was used as a loading control. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.19214), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody - BSA Free [NBP1-06640] -

JMJD3-induced H3K27me3 demethylation and up-regulated ADORA2A and C/EBP beta expressions in macrophage.A In LPS-stimulated macrophage, JMJD3 expression was increased while H3K27me3 was decreased (JMJD3: P < 0.001, control vs. LPS group; H3K27me3: P < 0.001, control vs. LPS group),using GSK-J4 inhibit JMJD3 with lower JMJD3 and higher H3K27me3 (JMJD3: P < 0.05, LPS vs. LPS + GSK-J4 group;H3K27me3: P < 0.001, LPS vs. LPS + GSK-J4 group) (n = 3 in each group). B In the LPS group, C/EBP beta expression was increased, and inhibiting JMJD3 could reduce C/EBP beta expression (C/EBP beta : P < 0.05, control vs. LPS group, P < 0.05, LPS vs. LPS+GSK-J4 group), (n = 3 in each group). C ADORA2A expression increased in LPS-stimulated macrophage and GSK-J4 made it downregulation (ADORA2A: P < 0.001, control vs. LPS group, P < 0.01, LPS vs. LPS + GSK-J4 group; H3K27me3: P < 0.001, control vs. LPS group, P < 0.01, LPS vs. LPS + GSK-J4 group) (n = 3 in each group). ns indicated no statistical significance, *P < 0.05, **P < 0.01, and ***P < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36456564), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody - BSA Free

Application
Recommended Usage

Flow Cytometry

reported in scientific literature (PMID 23772023)

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

reported in scientific literature (PMID 35177643)

Western Blot

2 ug/ml
Application Notes
This JMJD3 antibody is useful for Western blot where a band is seen at ~180 kDa.

Reviewed Applications

Read 4 reviews rated 4.3 using NBP1-06640 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lysine (K)-specific Demethylase 6B/KDM6B

JMJD3 is a histone demethylase that specifically demethylates trimethylated and dimethylated 'Lys-27' of histone H3, thereby playing a central role in histone code. It plays a central role in regulation of posterior development by regulating HOX gene expression. JMJD3 is involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation. It has shown to be essential to mammalian epidermal differentiation and is upregulated in prostate cancer.

Alternate Names

JMJD3, KDM6B, Lysine (K)specific Demethylase 6B

Gene Symbol

KDM6B

Additional Lysine (K)-specific Demethylase 6B/KDM6B Products

Product Documents for Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics

Citations for Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody - BSA Free

Customer Reviews for Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody - BSA Free (4)

4.3 out of 5
4 Customer Ratings
5 Stars
25%
4 Stars
75%
3 Stars
0%
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1 Stars
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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody
    Name: Robin Ren
    Application: Western Blot
    Sample Tested: Cell lysate, Sample Amount: 50ug-100ug protein
    Species: Mouse and Human
    Verified Customer | Posted 10/30/2018
    IL-4 stimulated Jmjd3 expression in Raw264.7 cell. From left to right, Day0, Day1,Day2,Day3.
    Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody - BSA Free NBP1-06640
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Human cancer cell
    Species: Human
    Verified Customer | Posted 09/07/2015
  • Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: PC12 whole cell lysate
    Species: Other
    Verified Customer | Posted 06/25/2014
  • Name: Andy Martens
    Application: Western Blot
    Sample Tested: 293T cells; lane 3 transfected with an miRNA
    Species: Human
    Verified Customer | Posted 11/16/2012
    Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody - BSA Free NBP1-06640

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Protocols

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FAQs for Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody - BSA Free

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  • Q: A customer is interested in using the KDM6B antibody, cat# NBP1-06640, with rat samples. Could you please BLAST the immunogen against the rat sequence, found on UniProt below: http://www.uniprot.org/uniprot/G3V9U4

    A:

    This antibody shares 100% sequence homology with the rat protein. Since this is higher that 90%, the species will be covered in our guarantee.

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