Lysyl tRNA synthetase Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87706

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Lysyl tRNA synthetase Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GMVKHITGSYKVTYHPDGPEGQAYDVDFTPPFRRINMVEELEKALGMKLPETNLFETEETRKILDDICVAKAVECPPPRTTARLLDKLVGEFLEVTCINPTFICDHPQIMSPLAKWHRSK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Lysyl tRNA synthetase Antibody - BSA Free

Western Blot: Lysyl tRNA synthetase Antibody [NBP1-87706]

Western Blot: Lysyl tRNA synthetase Antibody [NBP1-87706]

Western Blot: Lysyl tRNA synthetase Antibody [NBP1-87706] - Analysis using Anti-KARS antibody NBP1-87706 (A) shows similar pattern to independent antibody NBP1-87705 (B).
Immunocytochemistry/ Immunofluorescence: Lysyl tRNA synthetase Antibody [NBP1-87706]

Immunocytochemistry/ Immunofluorescence: Lysyl tRNA synthetase Antibody [NBP1-87706]

Immunocytochemistry/Immunofluorescence: Lysyl tRNA synthetase Antibody [NBP1-87706] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Lysyl tRNA synthetase Antibody [NBP1-87706]

Immunohistochemistry-Paraffin: Lysyl tRNA synthetase Antibody [NBP1-87706]

Immunohistochemistry-Paraffin: Lysyl tRNA synthetase Antibody [NBP1-87706] - Staining of human cerebral cortex.
Western Blot: Lysyl tRNA synthetase Antibody [NBP1-87706]

Western Blot: Lysyl tRNA synthetase Antibody [NBP1-87706]

Western Blot: Lysyl tRNA synthetase Antibody [NBP1-87706] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: Lysyl tRNA synthetase Antibody [NBP1-87706]

Immunohistochemistry-Paraffin: Lysyl tRNA synthetase Antibody [NBP1-87706]

Immunohistochemistry-Paraffin: Lysyl tRNA synthetase Antibody [NBP1-87706] - Staining of human hippocampus shows strong cytoplasmic positivity in neuronal cells.
Immunohistochemistry-Paraffin: Lysyl tRNA synthetase Antibody [NBP1-87706]

Immunohistochemistry-Paraffin: Lysyl tRNA synthetase Antibody [NBP1-87706]

Immunohistochemistry-Paraffin: Lysyl tRNA synthetase Antibody [NBP1-87706] - Staining of human cerebral cortex, colon, kidney and testis using Anti-KARS antibody NBP1-87706 (A) shows similar protein distribution across tissues to independent antibody NBP1-87705 (B).
Immunohistochemistry-Paraffin: Lysyl tRNA synthetase Antibody [NBP1-87706]

Immunohistochemistry-Paraffin: Lysyl tRNA synthetase Antibody [NBP1-87706]

Immunohistochemistry-Paraffin: Lysyl tRNA synthetase Antibody [NBP1-87706] - Staining of human colon.
Immunohistochemistry-Paraffin: Lysyl tRNA synthetase Antibody [NBP1-87706]

Immunohistochemistry-Paraffin: Lysyl tRNA synthetase Antibody [NBP1-87706]

Immunohistochemistry-Paraffin: Lysyl tRNA synthetase Antibody [NBP1-87706] - Staining of human kidney.
Immunohistochemistry-Paraffin: Lysyl tRNA synthetase Antibody [NBP1-87706]

Immunohistochemistry-Paraffin: Lysyl tRNA synthetase Antibody [NBP1-87706]

Immunohistochemistry-Paraffin: Lysyl tRNA synthetase Antibody [NBP1-87706] - Staining of human testis.

Applications for Lysyl tRNA synthetase Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lysyl tRNA synthetase

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis

Alternate Names

CMTRIB, EC 6.1.1, EC 6.1.1.6, KARS2, KIAA0070, KRS, lysine tRNA ligase, Lysine--tRNA ligase, lysRS, lysyl-tRNA synthetase

Gene Symbol

KARS1

Additional Lysyl tRNA synthetase Products

Product Documents for Lysyl tRNA synthetase Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Lysyl tRNA synthetase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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