M-CSFR/CD115 Antibody (6B9B9) - BSA Free

Novus Biologicals | Catalog # NBP2-37292

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 6B9B9

Format

BSA Free
View all M-CSF R/CD115 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human M-CSFR/CD115 (AA: 344-497) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

108 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for M-CSFR/CD115 Antibody (6B9B9) - BSA Free

Western Blot: M-CSFR/CD115 Antibody (6B9B9)BSA Free [NBP2-37292]

Western Blot: M-CSFR/CD115 Antibody (6B9B9)BSA Free [NBP2-37292]

Western Blot: M-CSFR/CD115 Antibody (6B9B9) [NBP2-37292] - Analysis using CSF1R mAb against HEK293 (1) and CSF1R (AA: 344-497)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: M-CSFR/CD115 Antibody (6B9B9) - BSA Free [NBP2-37292]

Immunocytochemistry/ Immunofluorescence: M-CSFR/CD115 Antibody (6B9B9) - BSA Free [NBP2-37292]

Immunocytochemistry/Immunofluorescence: M-CSFR/CD115 Antibody (6B9B9) [NBP2-37292] - Analysis of HepG2 cells using CSF1R mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry-Paraffin: M-CSFR/CD115 Antibody (6B9B9) - BSA Free [NBP2-37292]

Immunohistochemistry-Paraffin: M-CSFR/CD115 Antibody (6B9B9) - BSA Free [NBP2-37292]

Immunohistochemistry-Paraffin: M-CSFR/CD115 Antibody (6B9B9) [NBP2-37292] - Analysis of esophageal tissues using CSF1R mouse mAb with DAB staining.
Western Blot: M-CSFR/CD115 Antibody (6B9B9)BSA Free [NBP2-37292]

Western Blot: M-CSFR/CD115 Antibody (6B9B9)BSA Free [NBP2-37292]

Western Blot: M-CSFR/CD115 Antibody (6B9B9) [NBP2-37292] - Analysis using CSF1R mAb against human CSF1R (AA: 344-497) recombinant protein. (Expected MW is 43.3 kDa)
Immunohistochemistry-Paraffin: M-CSFR/CD115 Antibody (6B9B9) - BSA Free [NBP2-37292]

Immunohistochemistry-Paraffin: M-CSFR/CD115 Antibody (6B9B9) - BSA Free [NBP2-37292]

Immunohistochemistry-Paraffin: M-CSFR/CD115 Antibody (6B9B9) [NBP2-37292] - Analysis of pancreas tissues using CSF1R mouse mAb with DAB staining.
ELISA: M-CSFR/CD115 Antibody (6B9B9) - BSA Free [NBP2-37292]

ELISA: M-CSFR/CD115 Antibody (6B9B9) - BSA Free [NBP2-37292]

ELISA: M-CSFR/CD115 Antibody (6B9B9) [NBP2-37292] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for M-CSFR/CD115 Antibody (6B9B9) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: M-CSF R/CD115

MCSF Receptor is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is a member of the CSF1/PDGF receptor family of tyrosine protein kinases and contains 5 immunoglobulin like C2 type domains. CD115 is expressed by cells of the monocytic lineage and by progenitor cells. Mutations in this gene have been associated with a predisposition to myeloid malignancy.

Long Name

Macrophage Colony Stimulating Factor Receptor

Alternate Names

c-fms, CD115, CSF1R, M-CSFR, MCSFR

Gene Symbol

CSF1R

Additional M-CSF R/CD115 Products

Product Documents for M-CSFR/CD115 Antibody (6B9B9) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for M-CSFR/CD115 Antibody (6B9B9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for M-CSFR/CD115 Antibody (6B9B9) - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for M-CSFR/CD115 Antibody (6B9B9) - BSA Free

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  • Q: We need an antibody for immunofluorescence staining of macrophages for CSF-1 receptor.

    A: It looks like the cat# NBP2-37292 is the only CSF Receptor that has been validated in ICC/IF.

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Associated Pathways

Dendritic Cell Lineage Development Pathways Dendritic Cell Lineage Development Pathway Thumbnail