MAF1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-31617

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Bovine (98%), Rhesus Macaque (98%). Backed by our 100% Guarantee.

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MAF1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human MAF1. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (86%).

Localization

Nucleus, Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MAF1 Antibody - BSA Free

Western Blot: MAF1 Antibody [NBP1-31617]

Western Blot: MAF1 Antibody [NBP1-31617]

Western Blot: MAF1 Antibody [NBP1-31617] - Wild-type (WT) and MAF1 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MAF1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody
Immunocytochemistry/ Immunofluorescence: MAF1 Antibody [NBP1-31617]

Immunocytochemistry/ Immunofluorescence: MAF1 Antibody [NBP1-31617]

Immunocytochemistry/Immunofluorescence: MAF1 Antibody [NBP1-31617] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: MAF1 Antibody [NBP1-31617]

Immunohistochemistry-Paraffin: MAF1 Antibody [NBP1-31617]

Immunohistochemistry-Paraffin: MAF1 Antibody [NBP1-31617] - Human colon carcinoma. MAF1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Western Blot: MAF1 Antibody [NBP1-31617]

Western Blot: MAF1 Antibody [NBP1-31617]

Western Blot: MAF1 Antibody [NBP1-31617] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MAF1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Immunohistochemistry-Paraffin: MAF1 Antibody [NBP1-31617]

Immunohistochemistry-Paraffin: MAF1 Antibody [NBP1-31617]

Immunohistochemistry-Paraffin: MAF1 Antibody [NBP1-31617] - Human endometrial carcinoma. MAF1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Applications for MAF1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Simple Western

1:20

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MAF1

This gene encodes a protein that is similar to Maf1, a Saccharomyces cerevisiae protein highly conserved in eukaryotic cells. Yeast Maf1 is a negative effector of RNA polymerase III (Pol III). It responds to changes in the cellular environment and represses pol III transcription. Biochemical studies identified the initiation factor TFIIIB as a target for Maf1-dependent repression. [provided by RefSeq]

Alternate Names

DKFZp586G1123, homolog of yeast MAF1, MAF1 homolog (S. cerevisiae), MGC20332, MGC31779, MGC39758, repressor of RNA polymerase III transcription MAF1 homolog

Gene Symbol

MAF1

Additional MAF1 Products

Product Documents for MAF1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MAF1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for MAF1 Antibody - BSA Free

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Protocols

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