Malondialdehyde Antibody (11E3) - BSA Free

Western Blot: Malondialdehyde Antibody (11E3) [NBP2-59367]

Western Blot: Malondialdehyde Antibody (11E3) [NBP2-59367] - Western Blot analysis of Malondialdehyde-BSA Conjugate showing detection of 67 kDa Malondialdehyde protein using Mouse Anti-Malondialdehyde Monoclonal Antibody, Clone 11E3 (NBP2-59367). Lane 1: Molecular Weight Ladder (MW). Lane 2: Malondialdehyde-BSA (0.5 ug). Lane 3: Malondialdehyde-BSA (2.0 ug). Lane 4: BSA (0.5 ug). Lane 5: BSA (2.0 ug). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody (NBP2-59367) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.

Western Blot: Malondialdehyde Antibody (11E3) - BSA Free [NBP2-59367] -

S. aureus and E. coli infections induced lipid peroxides accumulation in infected bone microenvironment and BMSCs. A) Lipid peroxidation levels in bone marrow on Day 3 post‐infection in murine osteomyelitis model induced by S. aureus and E. coli were determined by WB analysis of 4‐HNE and MDA protein modifications. Each group contained four mice. B) Representative tissue immunofluorescence images of LepR, MDA, 4‐HNE, ACSL4, GPX4 in the uninfected murine femur and implant‐associated bone infection model induced by S. aureus and E. coli. Scale bar = 50 um. C) The schematic route of animal experiments and gating strategy were depicted. D) Lipid peroxide regulation by S. aureus and E. coli infections and Fer‐1 in CD45−CD31−Ter119−LepR+ BMSCs were analyzed in murine osteomyelitis model by Liperfluo staining and analyzed by flow cytometry. E,F) Quantification of median fluorescent intensity in flow cytometry analysis in (D). Values are means +/- SDs. Each group contained six mice. Multiple comparison was performed by one‐way analysis of variance (ANOVA) with Tukey's post‐hoc analysis. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39166412), licensed under a CC-BY license. Not internally tested by Novus Biologicals.