Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Bovine (92%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Antibody was raised against a 12 amino acid synthetic peptide from near the carboxy terminus of human MANF. The immunogen is located within the last 50 amino acids of MANF. Amino Acid Squence: KYAPKAASARTD
Specificity
This antibody does not cross-react with CDNF.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit MANF Antibody - BSA Free (NBP1-76836) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for MANF Antibody - BSA Free
Western Blot: MANF Antibody - BSA Free [NBP1-76836] -
Western Blot: MANF Antibody - BSA Free [NBP1-76836] - KO Validation in HEK293T Cells.Loading: 10 ug of HEK293T WT cell lysates or MANF KO cell lysates. Antibodies: MANF (1 ug/mL) and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.![Western Blot: MANF AntibodyBSA Free [NBP1-76836]](https://resources.rndsystems.com/images/products/MANF-Antibody-Western-Blot-NBP1-76836-img0007.jpg "Western Blot: MANF AntibodyBSA Free [NBP1-76836]")
Western Blot: MANF AntibodyBSA Free [NBP1-76836]
Western Blot: MANF Antibody [NBP1-76836] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human MANF recombinant protein per lane. Antibodies: MANF NBP1-76836 (Lane 1: 0.125 ug /mL, Lane 2: 0.25 ug/mL and Lane 3: 0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 55kDa.![Western Blot: MANF AntibodyBSA Free [NBP1-76836]](https://resources.rndsystems.com/images/products/MANF-Antibody-Western-Blot-NBP1-76836-img0006.jpg "Western Blot: MANF AntibodyBSA Free [NBP1-76836]")
Western Blot: MANF AntibodyBSA Free [NBP1-76836]
Western Blot: MANF Antibody [NBP1-76836] - Western Blot Validation in Human, Mouse (NIH/3T3) and Rat (YB2/0) Cell Lines.Loading: 15 ug of lysates per lane. Antibodies: MANF NBP1-76836 (2 ug /mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: MANF Antibody - BSA Free [NBP1-76836] -
Western Blot: MANF Antibody - BSA Free [NBP1-76836] - Validation in Human Tissues.Loading: 15 ug of lysates per lane.Antibodies: MANF, (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: MANF Antibody - BSA Free [NBP1-76836] -
Western Blot: MANF Antibody - BSA Free [NBP1-76836] - Validation in Rat Liver.Loading: 15 ug of lysates per lane.Antibodies: MANF, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 1 ug/mLLane 2: 2 ug/mLLane 3: 4 ug/mL
Western Blot: MANF Antibody - BSA Free [NBP1-76836] -
Western Blot: MANF Antibody - BSA Free [NBP1-76836] - Validation in Mouse TissuesLoading: 15 ug of lysates per lane.Antibodies: MANF, (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunohistochemistry: MANF Antibody - BSA Free [NBP1-76836] -
Immunohistochemistry: MANF Antibody - BSA Free [NBP1-76836] - Validation of MANF in Human Brain Tissue.Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-MANF antibody at 2.5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunocytochemistry/ Immunofluorescence: MANF Antibody - BSA Free [NBP1-76836] -
Immunocytochemistry/ Immunofluorescence: MANF Antibody - BSA Free [NBP1-76836] - Validation of MANF in Rat Liver.Immunofluorescent analysis of 4% paraformaldehyde-fixed rat liver cells labeling MANF with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).
Immunocytochemistry/ Immunofluorescence: MANF Antibody - BSA Free [NBP1-76836] -
Immunocytochemistry/ Immunofluorescence: MANF Antibody - BSA Free [NBP1-76836] - Validation of MANF in Mouse Pancreas. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse pancreas cells labeling MANF at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).
Immunocytochemistry/ Immunofluorescence: MANF Antibody - BSA Free [NBP1-76836] -
Immunocytochemistry/ Immunofluorescence: MANF Antibody - BSA Free [NBP1-76836] - Validation of MANF in Human Liver.Immunofluorescent analysis of 4% paraformaldehyde-fixed human liver cells labeling MANF at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).
Immunocytochemistry/ Immunofluorescence: MANF Antibody - BSA Free [NBP1-76836] -
Immunocytochemistry/ Immunofluorescence: MANF Antibody - BSA Free [NBP1-76836] - Validation of MANF in Human Colon.Immunofluorescent analysis of 4% paraformaldehyde-fixed human colon cells labeling MANF with at 5 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).
Immunocytochemistry/ Immunofluorescence: MANF Antibody - BSA Free [NBP1-76836] -
Immunocytochemistry/ Immunofluorescence: MANF Antibody - BSA Free [NBP1-76836] - Validation of MANF in Human Cervix.Immunofluorescent analysis of 4% paraformaldehyde-fixed human cervix cells labeling MANF at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).Applications for MANF Antibody - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
20 ug/ml
Immunohistochemistry
2.5 ug/mL
Western Blot
0.125-2 ug/mL
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MANF
Long Name
Mesencephalic Astrocyte-derived Neurotrphic Factor
Alternate Names
ARMET, ARP
Gene Symbol
MANF
UniProt
Additional MANF Products
Product Documents for MANF Antibody - BSA Free
Product Specific Notices for MANF Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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