MAP3K12 Antibody (HL3423)

Novus Biologicals | Catalog # NBP3-48811

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL3423
View all MAP3K12 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human MAP3K12.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MAP3K12 Antibody (HL3423)

MAP3K12 Antibody (HL3423)

Immunocytochemistry/ Immunofluorescence: MAP3K12 Antibody (HL3423) [NBP3-48811] -

MAP3K12 antibody [HL3423] detects MAP3K12 protein by immunofluorescent analysis.
Sample: Neuro2A cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: MAP3K12 stained by MAP3K12 antibody [HL3423] (NBP3-48811) diluted at 1:500.
Blue: Fluoroshield with DAPI.
MAP3K12 Antibody (HL3423)

Western Blot: MAP3K12 Antibody (HL3423) [NBP3-48811] -

Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAP3K12 antibody [HL3423] (NBP3-48811) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
MAP3K12 Antibody (HL3423)

Immunohistochemistry-Paraffin: MAP3K12 Antibody (HL3423) [NBP3-48811] -

MAP3K12 antibody [HL3423] detects MAP3K12 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
Green: MAP3K12 stained by MAP3K12 antibody [HL3423] (NBP3-48811) diluted at 1:200.
Red: NF-H antibody [GT114] diluted at 1:500.
Blue: Hoechst 33342 staining.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min
MAP3K12 Antibody (HL3423)

Immunohistochemistry-Paraffin: MAP3K12 Antibody (HL3423) [NBP3-48811] -

MAP3K12 antibody [HL3423] detects MAP3K12 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
MAP3K12 stained by MAP3K12 antibody [HL3423] (NBP3-48811) diluted at 1:200.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min
MAP3K12 Antibody (HL3423)

Immunohistochemistry-Paraffin: MAP3K12 Antibody (HL3423) [NBP3-48811] -

MAP3K12 antibody [HL3423] detects MAP3K12 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
MAP3K12 stained by MAP3K12 antibody [HL3423] (NBP3-48811) diluted at 1:200.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min
MAP3K12 Antibody (HL3423)

Immunohistochemistry-Paraffin: MAP3K12 Antibody (HL3423) [NBP3-48811] -

MAP3K12 antibody [HL3423] detects MAP3K12 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
MAP3K12 stained by MAP3K12 antibody [HL3423] (NBP3-48811) diluted at 1:100.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min
MAP3K12 Antibody (HL3423)

Immunohistochemistry-Paraffin: MAP3K12 Antibody (HL3423) [NBP3-48811] -

MAP3K12 antibody [HL3423] detects MAP3K12 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat hippocampus.
MAP3K12 stained by MAP3K12 antibody [HL3423] (NBP3-48811) diluted at 1:100.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

Applications for MAP3K12 Antibody (HL3423)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MAP3K12

The protein encoded by the MAP3K12 gene is a member of serine/threonine protein kinase family. This kinase contains a leucine-zipper domain, and is predominately expressed in neuronal cells. The phosphorylation state of this kinase in synaptic terminals was shown to be regulated by membrane depolarization via calcineurin. This kinase forms heterodimers with leucine zipper containing transcription factors, such as cAMP responsive element binding protein (CREB) and MYC, and thus may play a regulatory role in PKA or retinoic acid induced neuronal differentiation. (provided by RefSeq)

Alternate Names

DLKEC 2.7.11.25, Dual leucine zipper bearing kinase, EC 2.7.11, Leucine-zipper protein kinase, MAPK-upstream kinase, MEKK12, mitogen-activated protein kinase kinase kinase 12, Mixed lineage kinase, MUKdual leucine zipper kinase DLK, protein kinase MUK, ZPKleucine zipper protein kinase, ZPKP1

Gene Symbol

MAP3K12

Additional MAP3K12 Products

Product Documents for MAP3K12 Antibody (HL3423)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for MAP3K12 Antibody (HL3423)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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