Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry Free-Floating, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry Free-Floating, ELISA, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MBP Primary Antibodies »

Product Specifications

Immunogen

Reacts with residues 49-62 of Isoforms 5 and 6 of the human protein. The immunogen corresponds to Isoform 1 between amino acids 182-195 of the 33 kDa isoform. The antibody also detects mouse and rat MBP protein. The antibody primarily detects a 22 kDa band.

Reactivity Notes

Human, mouse and rat.

Marker

Oligodendrocyte Marker, Myelin Marker

Specificity

Myelin Basic Protein, Reacts with residues 49-62 of the 33 kDa human, mouse and rat MBP protein. Antibody detects primarily the 22 kDa isoform.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MBP Antibody - BSA Free

Western Blot: MBP AntibodyBSA Free [NB110-79873]

Western Blot: MBP AntibodyBSA Free [NB110-79873]

Western Blot: MBP Antibody [NB110-79873] - WB analysis of Myelin Basic Protein (MBP) in 10ug of normal brain lysate with antibody NB110-79873 at 1:500 dilution.
MBP Antibody - BSA Free

Simple Western: MBP Antibody [NB110-79873] -

Simple Western: MBP Antibody [NB110-79873] - Simple Western lane view shows a specific band for MBP in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image generated by internal validation.

Applications for MBP Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry-Paraffin

1:100-1:200

Simple Western

1:50

Western Blot

1:500-1:1000
Application Notes

This antibody is useful in ELISA, Immunocytochemistry, Immunohistochemistry 1:100-1:200 and Western Blot. In WB a band is seen at approx. 22 kDa representing MBP. Use in Immunohistochemistry free-floating reported in scientific literature (PMID 26616873).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in Human Brain lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:50, apparent MW was 30 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MBP

Myelin Basic Protein (MBP) functions in the nervous system in myelination and is expressed in oligodendrocytes following differentiation. There are numerous isoforms generated by differential splicing events and post-translational modifications that have specialized functions. These functions include participation in signaling pathways prior to myelination, myelination or the re-myelination process following neural injury.

Long Name

Myelin Basic Protein

Alternate Names

Hmbpr, mld, shi

Entrez Gene IDs

4155 (Human); 17196 (Mouse); 24547 (Rat)

Gene Symbol

MBP

UniProt

P02686, P04370 (Mouse), P02688 (Rat)

Additional MBP Products

Product Documents for MBP Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for MBP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Glial Lineage Markers

Citations for MBP Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for MBP Antibody - BSA Free

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  • Q: Is your antibody NB110-79873 indicated for IHC-Fr?

    A: NB110-79873 has been validated in IHC-Paraffin sections. We have not yet tested this antibody in frozen tissue sections.

  • Q: We have a customer looking for an MBP antibody to stain FFPE mouse brain sections. I found NB110-79873. Do you know if it has been specifically tested in IHC with mouse?

    A: We will guarantee this antibody for use in IHC-P for mouse samples. We had a collaborator verify this product for IHC so we don't have any specific data I can provide. The human and mouse sequence for the epitope that is provided (DRGAPKRGSGKDSH) are 100% identical. Obviously if it doesn't work for your customer we will refund or replace the product.

  • Q: Is your antibody NB110-79873 indicated for IHC-Fr?

    A: NB110-79873 has been validated in IHC-Paraffin sections. We have not yet tested this antibody in frozen tissue sections.

  • Q: We have a customer looking for an MBP antibody to stain FFPE mouse brain sections. I found NB110-79873. Do you know if it has been specifically tested in IHC with mouse?

    A: We will guarantee this antibody for use in IHC-P for mouse samples. We had a collaborator verify this product for IHC so we don't have any specific data I can provide. The human and mouse sequence for the epitope that is provided (DRGAPKRGSGKDSH) are 100% identical. Obviously if it doesn't work for your customer we will refund or replace the product.

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