Mca-PLAQAV-Dpa-RSSSR-NH2 Fluorogenic Peptide Substrate

Substrate for TACE, ADAM9, and ADAM10
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R&D Systems Fluorogenic Peptide Substrates
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Citations (19)
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Mca-PLAQAV-Dpa-RSSSR-NH2 Fluorogenic Peptide Substrate Summary

Product Specifications

>95%, by HPLC.
The peptide substrate contains a highly fluorescent 7-methoxycoumarin group that is efficiently quenched by resonance energy transfer to the 2,4-dinitrophenyl group. It can be used to measure the activities of peptidases that are capable of cleaving an amide bond between the fluorescent group and the quencher group, causing an increase in fluorescence. It is an excellent substrate for tumor necrosis factor-alpha converting enzyme (TACE or ADAM17) and related enzymes such as ADAM8, ADAM9 and ADAM10. The peptide sequence is derived from the pro tumor necrosis factor-alpha (TNF-alpha ). The cleavage site by TACE/ADAM17 and ADAM10 is the peptide bond between Ala and Val.

Mca: (7-Methoxycoumarin-4-yl) acetyl, Dpa: N-3-(2, 4-Dinitrophenyl)-L-2,3-diaminopropionyl.
Predicted Molecular Mass
1638 Da

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Samples are stable for up to twelve months from date of receipt at -20° C to -70° C. The substrate can be aliquoted and stored -20° C to -70° C in a manual defrost freezer for six months. Avoid repeated freeze-thaw cycles. Protect from exposure to direct light.
Reconstitution Calculator

Reconstitution Calculator

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Citations for Mca-PLAQAV-Dpa-RSSSR-NH2 Fluorogenic Peptide Substrate

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. HIV-1 release requires Nef-induced caspase activation
    Authors: J Segura, J Ireland, Z Zou, G Roth, J Buchwald, TJ Shen, E Fischer, S Moir, TW Chun, PD Sun
    PLoS ONE, 2023-02-13;18(2):e0281087.
    Species: Human
    Sample Types: Peptide
    Applications: Bioassay
  2. Alpha-1 Antitrypsin Reduces Disease Progression in a Mouse Model of Charcot-Marie-Tooth Type 1A: A Role for Decreased Inflammation and ADAM-17 Inhibition
    Authors: N Zhukovsky, M Silvano, T Filloux, S Gonzalez, KH Krause
    International Journal of Molecular Sciences, 2022-07-03;23(13):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  3. Regulation of the Receptor Tyrosine Kinase AXL in Response to Therapy and Its Role in Therapy Resistance in Glioblastoma
    Authors: L Scherschin, M Prem, I Kremenetsk, I Tinhofer, P Vajkoczy, AG Karbe, JS Onken
    International Journal of Molecular Sciences, 2022-01-17;23(2):.
    Species: Human
    Sample Types: Cell Lysates
  4. Inhibitory monoclonal antibody targeting ADAM17 expressed on cancer cells
    Authors: N Saha, K Xu, Z Zhu, D Robev, T Kalidindi, Y Xu, J Himanen, E de Stanchi, NVK Pillarsett, DS Dimitrov, DB Nikolov
    Translational Oncology, 2021-11-09;15(1):101265.
    Species: Human
    Sample Types: Peptide
    Applications: Bioassay
  5. Oncogenic KRAS promotes growth of lung cancer cells expressing SLC3A2-NRG1 fusion via ADAM17-mediated shedding of NRG1
    Authors: DH Shin, SH Kim, M Choi, YK Bae, C Han, BK Choi, SS Kim, JY Han
    Oncogene, 2021-11-06;0(0):.
    Species: Human
    Sample Types: Recombinant Proteins
    Applications: Bioassay
  6. Macrophage metabolic reprogramming aggravates aortic dissection through the HIF1alpha-ADAM17 pathway?
    Authors: G Lian, X Li, L Zhang, Y Zhang, L Sun, X Zhang, H Liu, Y Pang, W Kong, T Zhang, X Wang, C Jiang
    EBioMedicine, 2019-10-19;0(0):.
    Species: Mouse
    Sample Types: Protein
    Applications: Bioassay
  7. Novel Arginine-containing Macrocyclic MMP Inhibitors: Synthesis, 99mTc-labeling, and Evaluation
    Authors: Y Ye, J Toczek, K Gona, HY Kim, J Han, M Razavian, R Golestani, J Zhang, TL Wu, M Ghosh, JJ Jung, MM Sadeghi
    Sci Rep, 2018-08-03;8(1):11647.
    Species: Human
    Sample Types: Recombinant Protein
  8. Tumor necrosis factor ?-converting enzyme inhibitor attenuates lipopolysaccharide-induced reactive oxygen species and mitogen-activated protein kinase expression in human renal proximal tubule epithelial cells
    Authors: EH Bae, IJ Kim, HS Choi, HY Kim, CS Kim, SK Ma, IS Kim, SW Kim
    Korean J. Physiol. Pharmacol., 2018-02-23;22(2):135-143.
  9. Macrocyclic ?-defensins suppress tumor necrosis factor-? (TNF-?) shedding by inhibition of TNF-? converting enzyme
    Authors: JB Schaal, T Maretzky, DQ Tran, PA Tran, P Tongaonkar, CP Blobel, AJ Ouellette, ME Selsted
    J. Biol. Chem., 2018-01-09;0(0):.
    Applications: Bioassay
  10. Dysregulation of the ADAM17/Notch signalling pathways in endometriosis: from oxidative stress to fibrosis
    Authors: I González-F, P Santulli, S Chouzenoux, F Carmona, C Chapron, F Batteux
    Mol. Hum. Reprod., 2017-07-01;0(0):1-12.
    Applications: Bioassay
  11. Harnessing the natural inhibitory domain to control TNF? Converting Enzyme (TACE) activity in vivo
    Sci Rep, 2016-12-16;6(0):35598.
  12. ADAM10 Cell Surface Expression but Not Activity Is Critical for Staphylococcus aureus ?-Hemolysin-Mediated Activation of the NLRP3 Inflammasome in Human Monocytes.
    Authors: Ejiofor a D Ezekwe, Chengyu Weng, Joseph A Duncan
    Toxins, 2016-03-30;0(0):2072-6651.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. ADAM17-mediated shedding of FcgammaRIIIA on human NK cells: identification of the cleavage site and relationship with activation.
    Authors: Lajoie L, Congy-Jolivet N, Bolzec A, Gouilleux-Gruart V, Sicard E, Sung H, Peiretti F, Moreau T, Vie H, Clemenceau B, Thibault G
    J Immunol, 2013-12-13;192(2):741-51.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  14. Canonical transforming growth factor-beta signaling regulates disintegrin metalloprotease expression in experimental renal fibrosis via miR-29.
    Authors: Ramdas V, McBride M, Denby L, Baker A
    Am J Pathol, 2013-10-06;183(6):1885-96.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Bioassay
  15. Pathologic shear triggers shedding of vascular receptors: a novel mechanism for down-regulation of platelet glycoprotein VI in stenosed coronary vessels.
    Authors: Al-Tamimi M, Tan CW, Qiao J, Pennings GJ, Javadzadegan A, Yong AS, Arthur JF, Davis AK, Jing J, Mu FT, Hamilton JR, Jackson SP, Ludwig A, Berndt MC, Ward CM, Kritharides L, Andrews RK, Gardiner EE
    Blood, 2012-03-19;119(18):4311-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: Enzyme Assay
  16. Elevated CSF levels of TACE activity and soluble TNF receptors in subjects with mild cognitive impairment and patients with Alzheimer's disease.
    Authors: Jiang H, Hampel H, Prvulovic D, Wallin A, Blennow K, Li R, Shen Y
    Mol Neurodegener, 2011-10-06;6(0):69.
    Species: Human
    Sample Types: CSF
  17. Unsaturated fatty acids drive disintegrin and metalloproteinase (ADAM)-dependent cell adhesion, proliferation, and migration by modulating membrane fluidity.
    Authors: Reiss K, Cornelsen I, Husmann M
    J. Biol. Chem., 2011-06-03;286(30):26931-42.
    Species: Rabbit
    Sample Types: Whole Cells
  18. Microparticles of human atherosclerotic plaques enhance the shedding of the tumor necrosis factor-alpha converting enzyme/ADAM17 substrates, tumor necrosis factor and tumor necrosis factor receptor-1.
    Authors: Canault M, Leroyer AS, Peiretti F, Leseche G, Tedgui A, Bonardo B, Alessi MC, Boulanger CM, Nalbone G
    Am. J. Pathol., 2007-09-14;171(5):1713-23.
    Species: Human
    Sample Types: Biofilm
    Applications: Bioassay
  19. Targeting ADAM-mediated ligand cleavage to inhibit HER3 and EGFR pathways in non-small cell lung cancer.
    Authors: Zhou BB, Peyton M, He B, Liu C, Girard L, Caudler E, Lo Y, Baribaud F, Mikami I, Reguart N, Yang G, Li Y, Yao W, Vaddi K, Gazdar AF, Friedman SM, Jablons DM, Newton RC, Fridman JS, Minna JD, Scherle PA
    Cancer Cell, 2006-07-01;10(1):39-50.
    Species: Human
    Sample Types: Protein


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Mca-PLAQAV-Dpa-RSSSR-NH2 Fluorogenic Peptide Substrate
By Anonymous on 05/22/2017
Application: In vitro bioactivity in cell culture