Mca-PLAQAV-Dpa-RSSSR-NH2 Fluorogenic Peptide Substrate

R&D Systems | Catalog # ES003

Substrate for TACE, ADAM9, and ADAM10
R&D Systems
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Key Product Details

Conjugate

7-Methoxycoumarin

Applications

Bioactivity
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Product Specifications

Source


Mca-Pro-Leu-Ala-Gln-Ala-Val-Dpa-Arg-Ser-Ser-Ser-Arg-NH2
Mca: (7-Methoxycoumarin-4-yl) acetyl, Dpa: N-3-(2, 4-Dinitrophenyl)-L-2,3-diaminopropionyl.

Purity

>95%, by HPLC.

Predicted Molecular Mass

1638 Da

Activity

The peptide substrate contains a highly fluorescent 7-methoxycoumarin group that is efficiently quenched by resonance energy transfer to the 2,4-dinitrophenyl group. It can be used to measure the activities of peptidases that are capable of cleaving an amide bond between the fluorescent group and the quencher group, causing an increase in fluorescence.

It is an excellent substrate for tumor necrosis factor-alpha converting enzyme (TACE or ADAM17) and related enzymes such as ADAM8, ADAM9 and ADAM10. The peptide sequence is derived from the pro tumor necrosis factor-alpha (TNF-alpha ). The cleavage site by TACE/ADAM17 and ADAM10 is the peptide bond between Ala and Val.

Reviewed Applications

Read 1 review rated 5 using ES003 in the following applications:

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Formulation, Preparation, and Storage

ES003
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Samples are stable for up to twelve months from date of receipt at -20° C to -70° C. The substrate can be aliquoted and stored -20° C to -70° C in a manual defrost freezer for six months. Avoid repeated freeze-thaw cycles. Protect from exposure to direct light.

Product Documents for Mca-PLAQAV-Dpa-RSSSR-NH2 Fluorogenic Peptide Substrate

Certificate of Analysis

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Product Specific Notices for Mca-PLAQAV-Dpa-RSSSR-NH2 Fluorogenic Peptide Substrate

For research use only

Citations for Mca-PLAQAV-Dpa-RSSSR-NH2 Fluorogenic Peptide Substrate

Customer Reviews for Mca-PLAQAV-Dpa-RSSSR-NH2 Fluorogenic Peptide Substrate (1)

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  • Mca-PLAQAV-Dpa-RSSSR-NH2 Fluorogenic Peptide Substrate
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 05/22/2017

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