MCAM/CD146 Antibody - Azide Free

Novus Biologicals | Catalog # NBP2-15778

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free
View all MCAM/CD146 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human MCAM/CD146. The exact sequence is proprietary.

Localization

Membrane; Single-pass type I membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

72 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MCAM/CD146 Antibody - Azide Free

Western Blot: MCAM/CD146 Antibody [NBP2-15778]

Western Blot: MCAM/CD146 Antibody [NBP2-15778]

Western Blot: MCAM/CD146 Antibody [NBP2-15778] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with CD146 antibody [C1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: MCAM/CD146 Antibody [NBP2-15778]

Western Blot: MCAM/CD146 Antibody [NBP2-15778]

Western Blot: MCAM/CD146 Antibody [NBP2-15778] - A. 50 ug rat liver extract 7.5 % SDS-PAGE CD146 antibody [C1C3] dilution: 1:1000
Western Blot: MCAM/CD146 Antibody [NBP2-15778]

Western Blot: MCAM/CD146 Antibody [NBP2-15778]

Western Blot: MCAM/CD146 Antibody [NBP2-15778] - A. 30 ug HUVEC whole cell lysate/extract 7.5 % SDS-PAGE CD146 antibody [C1C3] dilution: 1:10000
Immunohistochemistry-Paraffin: MCAM/CD146 Antibody [NBP2-15778]

Immunohistochemistry-Paraffin: MCAM/CD146 Antibody [NBP2-15778]

Immunohistochemistry-Paraffin: MCAM/CD146 Antibody [NBP2-15778] - J5 xenograft, using CD146 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: MCAM/CD146 Antibody [NBP2-15778]

Immunohistochemistry-Paraffin: MCAM/CD146 Antibody [NBP2-15778]

Immunohistochemistry-Paraffin: MCAM/CD146 Antibody [NBP2-15778] - Mouse colon. CD146 antibody [C1C3] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: MCAM/CD146 Antibody [NBP2-15778]

Immunohistochemistry-Paraffin: MCAM/CD146 Antibody [NBP2-15778]

Immunohistochemistry-Paraffin: MCAM/CD146 Antibody [NBP2-15778] - Mouse skin. CD146 antibody [C1C3] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Knockout Validated: MCAM/CD146 Antibody [NBP2-15778]

Western Blot: MCAM/CD146 Antibody [NBP2-15778]

Western Blot: MCAM/CD146 Antibody [NBP2-15778] - Wild-type (WT) and CD146 knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with CD146 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
MCAM/CD146 Antibody - Azide Free

Immunohistochemistry-Paraffin: MCAM/CD146 Antibody [NBP2-15778] -

CD146 antibody [C1C3] detects CD146 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse placenta.
Green: CD146 stained by CD146 antibody [C1C3] (NBP2-15778) diluted at 1:250.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Blue: Fluoroshield with DAPI.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
MCAM/CD146 Antibody - Azide Free

Immunocytochemistry/ Immunofluorescence: MCAM/CD146 Antibody [NBP2-15778] -

CD146 antibody [C1C3] detects CD146 protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: HUVEC cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CD146 protein stained by CD146 antibody [C1C3] (NBP2-15778) diluted at 1:500.
Blue: Hoechst 33342 staining.

Applications for MCAM/CD146 Antibody - Azide Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Format

Azide Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MCAM/CD146

Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2, and a transient increase in the intracellular calcium concentration.

Long Name

Melanoma Cell Adhesion Molecule

Alternate Names

CD146, L-Gicerin, MUC18

Gene Symbol

MCAM

UniProt

P43121 (Human)

Additional MCAM/CD146 Products

Product Documents for MCAM/CD146 Antibody - Azide Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for MCAM/CD146 Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for MCAM/CD146 Antibody - Azide Free

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Protocols

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