MCT1/SLC16A1 Antibody - BSA Free

Immunohistochemistry-Paraffin: MCT1/SLC16A1 Antibody - BSA Free [NBP1-59656]

Immunohistochemistry-Paraffin: MCT1/SLC16A1 Antibody [NBP1-59656] - Staining with Monocarboxylic acid transporter 1 antibody. Strong staining of lumen and crypt cells was observed with weaker cytoplasmic staining observed in the submucosa of mouse intestine.

Immunocytochemistry/ Immunofluorescence: MCT1/SLC16A1 Antibody - BSA Free [NBP1-59656]

Immunocytochemistry/Immunofluorescence: MCT1/SLC16A1 Antibody [NBP1-59656] - Monocarboxylic acid transporter 1 antibody was tested in HeLa cells with DyLight488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). General cytoplasmic and membrane staining was observed.

Western Blot: MCT1/SLC16A1 AntibodyBSA Free [NBP1-59656]

Western Blot: MCT1/SLC16A1 Antibody [NBP1-59656] - Monocarboxylic acid transporter 1 Antibody [NBP1-59656] - Jurkat cell lysate, concentration 2.5 ug/mL.

MCT1/SLC16A1 in HepG2 Human Cell Line.

MCT1/SLC16A1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Rabbit anti- MCT1/SLC16A1 Affinity Purified Polyclonal Antibody conjugated to Alexa Fluor® 647 (Catalog # NBP1-59656AF647) (light blue) at 10 µg/mL overnight at 4C. Cells were counterstained with DAPI (dark blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunocytochemistry/ Immunofluorescence: MCT1/SLC16A1 Antibody - BSA Free [NBP1-59656] -

Immunocytochemistry/ Immunofluorescence: MCT1/SLC16A1 Antibody - BSA Free [NBP1-59656] - Immunofluorescence detection of ion transporters & water transporters in differentiated & CST- SPs. Na+/K+-ATP isoform ATP1A1, water transporter Aquaporin 1, SLC4A11, Bicarbonate transporter NBCe1, Na+-H+ exchanger NHE1 & ZO-1 as an endothelial barrier to complements fluid transport, were all selectively expressed in differentiated SPs, but were almost null in the cell-state-transitioned SPs. Monocarboxylic acid transporter 4 (MCT4) was expressed in the former, but not in the latter SPs, whereas the isomer MCT1 was selectively expressed in the latter SPs, but not in the former SPs. Differentiated SPs were obtained from the same donor (two eyes, right & left mixed) & were incubated in the presence of 10-µM Y27632 (differentiated SPs, CD44-/ + proportion: 86.7%). CST-SPs were obtained from the separate donor (two eyes, right & left mixed) & were incubated in the presence of 10-µM Y27632 + 1-µM SB431542 (SB4) + 10-µM SB203580 (SB2) + 5-ng/mL epidermal growth factor (EGF). The differentiated SPs were fixed at 37 days of the second passage, & the CST SPs were fixed at 38 days of the second passage. Bars: 100 µm. Experiments were repeated four times. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35428816), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: MCT1/SLC16A1 Antibody - BSA Free [NBP1-59656] -

Western Blot: MCT1/SLC16A1 Antibody - BSA Free [NBP1-59656] - Blockade of MCT1 inhibits the effects of optogenetic stimulation of sympathetic efferent fibers of BAT. (a) Cropped images of western blotting showing expression of MCT1 in the mitochondrial fractions (n = 2 mice). Full-length blots are presented in Supplementary Figure 2. VDAC: voltage-dependent anion channel, Cyt. C: cytochrome C, Cadhe: Cadherin (b) Pooled data from 6 mice showing changes in Slc16a1 (Mct1) mRNA expression with (filled square) & without (open circle) stimulation of sympathetic innervation of BAT (***p < 0.001, unpaired t-test). Data are shown as mean ± SEM. (c) Pooled data showing effects of blockade of the MCT1 on optogenetically induced increase in body temperature & glucose uptake (n = 5 mice). Data are shown as mean ± SEM. (d) Plot showing relative Slc16a1 (Mct1) mRNA expression in mice injected with control or Slc16a1 shRNA into BAT (n = 6 mice, ***p < 0.001, unpaired t-test). Data are shown as mean ± SEM. (e) Western blotting to show knockdown of the Mct1 gene in BAT injected with Mct1 shRNA. Cropped images of western blotting showing knockdown of MCT1 protein (upper panel). Plot showing relative expression of MCT1 protein (bottom panel, *p < 0.05, unpaired t-test). Data are shown as mean ± SEM. (f) Pooled data showing that mice injected with Slc16a1 (Mct1) shRNA into the BAT pad showed no response to optogenetic stimulation. Data are shown as mean ± SEM. Image collected & cropped by CiteAb from the following publication (https://www.nature.com/articles/s41598-018-25265-3), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: MCT1/SLC16A1 Antibody - BSA Free [NBP1-59656] -

Immunocytochemistry/ Immunofluorescence: MCT1/SLC16A1 Antibody - BSA Free [NBP1-59656] - Immunofluorescence detection of ion transporters & water transporters in differentiated & CST- SPs. Na+/K+-ATP isoform ATP1A1, water transporter Aquaporin 1, SLC4A11, Bicarbonate transporter NBCe1, Na+-H+ exchanger NHE1 & ZO-1 as an endothelial barrier to complements fluid transport, were all selectively expressed in differentiated SPs, but were almost null in the cell-state-transitioned SPs. Monocarboxylic acid transporter 4 (MCT4) was expressed in the former, but not in the latter SPs, whereas the isomer MCT1 was selectively expressed in the latter SPs, but not in the former SPs. Differentiated SPs were obtained from the same donor (two eyes, right & left mixed) & were incubated in the presence of 10-µM Y27632 (differentiated SPs, CD44-/ + proportion: 86.7%). CST-SPs were obtained from the separate donor (two eyes, right & left mixed) & were incubated in the presence of 10-µM Y27632 + 1-µM SB431542 (SB4) + 10-µM SB203580 (SB2) + 5-ng/mL epidermal growth factor (EGF). The differentiated SPs were fixed at 37 days of the second passage, & the CST SPs were fixed at 38 days of the second passage. Bars: 100 µm. Experiments were repeated four times. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35428816), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Simple Western: MCT1/SLC16A1 Antibody - BSA Free [NBP1-59656] -

Simple Western: MCT1/SLC16A1 Antibody - BSA Free [NBP1-59656] - CNOT3 is essential for beta -cell maturation & identity.a qPCR analysis of progenitor cells/dedifferentiation markers, normalized to the Gapdh mRNA level, in control & Cnot3 beta KO islets (n = 4–6). b Immunoblot analysis of ALDH1A3 in islet lysates from 8-week-old control & Cnot3 beta KO mice. This blot is a representative of three different blots. c Band quantification of an immunoblot of ALDH1A3 (n = 3) in Fig. 4b. d qPCR analysis of beta -cell-specific functional mRNAs expression categorized as beta -cell-specific transcription factors, glycolytic pathway, insulin granule maturation, & insulin secretion mRNAs, normalized to the Gapdh mRNA level, in control & Cnot3 beta KO islets (n = 3–7). e Co-immunofluorescence staining of MAFA (green), GLUT2 (green), & insulin (magenta) in pancreatic sections from 8-week-old control & Cnot3 beta KO mice. A scale bar represents 25 µm. Representative results from four 8-week-old mice from each genotype are shown. f qPCR analysis of immature beta -cell markers, normalized to the Gapdh mRNA level, in control & Cnot3 beta KO islets (n = 5–7). g Immunoblot analysis of MCT1 & LDHA in islet lysates from 8-week-old control & Cnot3 beta KO mice. This blot is a representative of three different blots. h Band quantification of immunoblot of MCT1 & LDHA (n = 3). Data are presented as mean ± SEM; *P < 0.05; **P < 0.01; ***P < 0.001, two-tailed Student’s t test. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32859966), licensed under a CC-BY license. Not internally tested by Novus Biologicals.