Key Product Details

Validated by

Tagged Protein Expression

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

ELISA, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MD2 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 13 amino acids near the center of human MD-2. The immunogen is located within the last 50 amino acids of MD-2. Amino Acid Sequence: CSFKGIKFSKGKYK

Specificity

Human MD-2 has 2 isoforms, including isoform 1 (160aa, 18kD) and isoform 2 (130aa, 15kD). Mouse MD-2 has one isoform (160aa, 18kD) and Rat MD-2 also has one isoform (160aa, 18kD). NBP1-77201 can detect human, mouse and rat.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit MD-2 Antibody - BSA Free (NBP1-77201) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-MD-2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for MD-2 Antibody - BSA Free

Western Blot: MD-2 AntibodyBSA Free [NBP1-77201]

Western Blot: MD-2 AntibodyBSA Free [NBP1-77201]

Western Blot: MD-2 Antibody [NBP1-77201] - Western Blot Validation in Rat Tissue Lysates. Loading: 15 ug of lysates per lane.Antibodies: MD-2, NBP1-77201 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: MD-2 AntibodyBSA Free [NBP1-77201]

Western Blot: MD-2 AntibodyBSA Free [NBP1-77201]

Western Blot: MD-2 Antibody [NBP1-77201] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human MD-2 recombinant protein per lane. Antibodies: MD-2, NBP1-77201 (Lane 1: 0.5 ug/mL, Lane 2: 1 ug/mL and Lane 3: 2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: MD-2 AntibodyBSA Free [NBP1-77201]

Western Blot: MD-2 AntibodyBSA Free [NBP1-77201]

Western Blot: MD-2 Antibody [NBP1-77201] - Western Blot Validation in Daudi Cell Lysate. Loading: 15 ug of lysates per lane.Antibodies: MD-2, NBP1-77201 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: MD-2 AntibodyBSA Free [NBP1-77201]

Western Blot: MD-2 AntibodyBSA Free [NBP1-77201]

Western Blot: MD-2 Antibody [NBP1-77201] - Western Blot Validation in Mouse Spleen Tissue Lysate. Loading: 15 ug of lysates per lane. Antibodies: MD-2, NBP1-77201 (Lane 1: 0.5 ug/mL and Lane 2: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
MD-2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: MD-2 Antibody - BSA Free [NBP1-77201] -

Immunocytochemistry/ Immunofluorescence: MD-2 Antibody - BSA Free [NBP1-77201] - Validation of MD-2 in Human Spleen Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen tissue labeling MD-2 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
Western Blot: MD-2 AntibodyBSA Free [NBP1-77201]

Western Blot: MD-2 AntibodyBSA Free [NBP1-77201]

Western Blot: MD-2 Antibody [NBP1-77201] - Western Blot Validation in Mouse Tissue Lysates. Loading: 15 ug of lysates per lane.Antibodies: MD-2, NBP1-77201 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
MD-2 Antibody - BSA Free

Western Blot: MD-2 Antibody - BSA Free [NBP1-77201] -

Western Blot: MD-2 Antibody - BSA Free [NBP1-77201] - Validation in Mouse Spleen Tissue LysateLoading: 15 ug of lysates per lane.Antibodies: MD-2, (Lane 1: 0.5 ug/mL and Lane 2: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
MD-2 Antibody - BSA Free

Immunohistochemistry: MD-2 Antibody - BSA Free [NBP1-77201] -

Immunohistochemistry: MD-2 Antibody - BSA Free [NBP1-77201] - Validation of MD-2 in Human Spleen Tissue. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-MD-2 antibody at 2.5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
MD-2 Antibody - BSA Free

Western Blot: MD-2 Antibody - BSA Free [NBP1-77201] -

Western Blot: MD-2 Antibody - BSA Free [NBP1-77201] - Validation in Rat Tissue Lysates.Loading: 15 ug of lysates per lane.Antibodies: MD-2, (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
MD-2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: MD-2 Antibody - BSA Free [NBP1-77201] -

Immunocytochemistry/ Immunofluorescence: MD-2 Antibody - BSA Free [NBP1-77201] - Validation of MD-2 in Rat Spleen Tissue.Immunofluorescent analysis of 4% paraformaldehyde-fixed rat spleen tissue labeling MD-2 with at 10 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
MD-2 Antibody - BSA Free

Immunohistochemistry: MD-2 Antibody - BSA Free [NBP1-77201] -

Immunohistochemistry: MD-2 Antibody - BSA Free [NBP1-77201] - Validation of MD-2 in Rat Spleen Tissue. Immunohistochemical analysis of paraffin-embedded rat spleen using anti-MD-2 antibody at 2 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
MD-2 Antibody - BSA Free

Western Blot: MD-2 Antibody - BSA Free [NBP1-77201] -

Western Blot: MD-2 Antibody - BSA Free [NBP1-77201] - Validation in Rat Spleen Tissue Lysate.Loading: 15 ug of lysates per lane.Antibodies: MD-2, (Lane 1: 0.5 ug/mL and Lane 2: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Applications for MD-2 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10-20 ug/mL

Immunohistochemistry

2-2.5 ug/mL

Immunohistochemistry-Paraffin

2 ug/ml

Western Blot

0.5-1 ug/mL

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: MD2

MD-2 is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs). TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses. TLR4, the major signaling receptor for lipopolysaccharide (LPS), requires the binding of MD-2 to its extracellular region for maximal response to LPS. The specificity of this response is determined by the species of MD-2; e.g., human MD-2 can cause mouse TLR4 to react to LPS analogs that are normally antagonistic to human but not mouse TLR4.

Long Name

Myeloid Differentiation Protein 2/Lymphocyte Antigen 96

Alternate Names

ESOP-1, LY96, MD-2

Gene Symbol

LY96

UniProt

NP_056179 (Human)

Additional MD2 Products

Product Documents for MD-2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MD-2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Monocyte Markers
Toll-Like Receptors

Citations for MD-2 Antibody - BSA Free

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Protocols

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