Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Chimpanzee (100%), Monkey (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MDC1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide, which represented a portion of human Mediator of DNA Damage Checkpoint Protein 1 encoded within exon 5 (NP_055456.1).

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26903517).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MDC1 Antibody - BSA Free

Western Blot: MDC1 Antibody [NB100-395]

Western Blot: MDC1 Antibody [NB100-395]

Western Blot: MDC1 Antibody [NB100-395] - Human lung cancer cell lines (UT) Untreated and (Cis) 10uM Cisplatin 16h. Image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: MDC1 Antibody [NB100-395]

Immunocytochemistry/ Immunofluorescence: MDC1 Antibody [NB100-395]

MDC1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-395-img0020.jpg
Western Blot: MDC1 Antibody [NB100-395]

Western Blot: MDC1 Antibody [NB100-395]

Western Blot: MDC1 Antibody [NB100-395] - Samples: Whole cell lysate (15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MDC1 antibody NB100-395 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: MDC1 Antibody [NB100-395]

Immunoprecipitation: MDC1 Antibody [NB100-395]

Immunoprecipitation: MDC1 Antibody [NB100-395] - Detection of human MDC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MDC1 antibody NB100-395 used for IP at 3 ug per reaction. MDC1 was also immunoprecipitated by two other rabbit anti-MDC1 antibodies. For blotting immunoprecipitated MDC1, NB100-395 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
MDC1 Antibody

Western Blot: MDC1 Antibody [NB100-395] -

Western Blot: MDC1 Antibody [NB100-395] - BRUCE UBC domain is required for NBS1 repair foci formation.U2OS-shBRUCE cells with stable expression of BRUCE or BRUCE mutants as indicated to the left were treated with DOX to knockdown endogenous BRUCE. After irradiation, cells were fixed & immunofluorescence stained for NBS1 (A) & quantified for cells with more than five nuclear foci (B). Bars, 10 μm. Error bars represent standard deviation from a triplicate of a representative experiment. DOX treated shBRUCE-U2OS cells were irradiated & the cell lysates subject to immunoblotting with the indicated antibodies showing the protein levels of ATM, MDC1 & NBS1 are not reduced post BRUCE knockdown (C). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26683461), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for MDC1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:250

Immunoprecipitation

2 - 5 ug/mg lysate

Western Blot

1:2000 - 1:10000
Application Notes
WB of lysates performed using standard western blot reagents and 4-8% SDS-PAGE. ICC/IF reactivity reported in (PMID: 26903517). MDC1 antibody validated for WB from a verified customer review.

Reviewed Applications

Read 2 reviews rated 4.5 using NB100-395 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MDC1

MDC1 works with H2AX to promote recruitment of repair proteins to the sites of DNA breaks and controls damage-induced cell-cycle arrest checkpoints. Cells that lack MDC1 are sensitive to ionizing radiation.

Long Name

Mediator of DNA-damage checkpoint 1

Alternate Names

NFBD1

Entrez Gene IDs

9656 (Human)

Gene Symbol

MDC1

UniProt

Q14676

Additional MDC1 Products

Product Documents for MDC1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for MDC1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MDC1 Antibody - BSA Free

Customer Reviews for MDC1 Antibody - BSA Free (2)

4.5 out of 5
2 Customer Ratings
5 Stars
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4 Stars
50%
3 Stars
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1 Stars
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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • MDC1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: human lung cancer cell line
    Species: Human
    Verified Customer | Posted 02/18/2019
    UT: untreated, Cis: 10uM Cisplatin 16h
    MDC1 Antibody - BSA Free NB100-395
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: Marsupial
    Species: Other
    Verified Customer | Posted 10/19/2015

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