MDR1/ABCB1 Antibody (SN06-42)

Novus Biologicals | Catalog # NBP2-67667

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SN06-42 expressed in HEK293
View all MDR1/ABCB1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein within Human MDR1/ABCB1 aa 373-730 / 1,280. (SwissProt: P08183 Human; SwissProt: P06795 Mouse; SwissProt: P43245 Rat)

Localization

Cell membrane.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MDR1/ABCB1 Antibody (SN06-42)

Western Blot: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667]

Western Blot: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667]

Western Blot: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Analysis of P Glycoprotein on mouse heart tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.
Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667]

Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667]

Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MDR1/ABCB1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667]

Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667]

Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Analysis of paraffin-embedded human liver tissue using anti-P Glycoprotein antibody. Counter stained with hematoxylin.
Immunohistochemistry-Frozen: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667]

Immunohistochemistry-Frozen: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667]

Immunohistochemistry-Frozen: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Mouse (B6) liver tissue. Fixed in ice-cold methanol for 10 minutes. Blocking in 10% serum and 1% BSA. Primary antibody at 5 ug/mL, overnight incubation. Secondary antibody at 2 ug/mL for 1.5 hour (Invitrogen A-11012). Mounting with Vector Vectashield with DAPI. Image from a verified customer review.
Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667]

Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667]

Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Analysis of paraffin-embedded mouse kidney tissue using anti-P Glycoprotein antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667]

Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667]

Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-MDR1/ABCB1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667]

Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667]

Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-MDR1/ABCB1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
MDR1/ABCB1 Antibody (SN06-42)

Immunocytochemistry/Immunofluorescence: Rabbit Monoclonal MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] -

Immunocytochemistry/Immunofluorescence: Rabbit Monoclonal MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Human brain microvascular endothelial cells stained for ABCB1, red and ciliary MDR1/ABCB1 green and nuclear DAPI blue. Image from a verified customer review.

Applications for MDR1/ABCB1 Antibody (SN06-42)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:1000
Application Notes
Use in ICC/IF reported in scientific literature (PMID: 31527311).

Reviewed Applications

Read 2 reviews rated 4.5 using NBP2-67667 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MDR1/ABCB1

MDR1 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). MDR1 is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. (provided by RefSeq)

Long Name

ATP-binding Cassette, Sub-family B (MDR/TAP), Member 1B

Alternate Names

ABC20, ABCB1, ABCB1B, CD243, CLCS, GP170, IBD13, PGY1

Gene Symbol

ABCB1

Additional MDR1/ABCB1 Products

Product Documents for MDR1/ABCB1 Antibody (SN06-42)

Certificate of Analysis

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Product Specific Notices for MDR1/ABCB1 Antibody (SN06-42)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MDR1/ABCB1 Antibody (SN06-42)

Customer Reviews for MDR1/ABCB1 Antibody (SN06-42) (2)

4.5 out of 5
2 Customer Ratings
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Customer Images

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  • MDR1/ABCB1 Antibody (SN06-42)
    Name: KARTHIKEYAN THIRUGNANAM
    Application: Immunocytochemistry
    Sample Tested: human brain microvascular endothelial cells
    Species: Human
    Verified Customer | Posted 01/23/2024
    Human brain microvascular endothelial cells stained for ABCB1, red and ciliary ARL13B green and nuclear DAPI blue.
    MDR1/ABCB1 Antibody (SN06-42) NBP2-67667
  • MDR1/ABCB1 Antibody (SN06-42)
    Name: Milan Prajapati
    Application: IF-Freshly embedded-Frozen
    Sample Tested: Liver
    Species: Mouse-B6
    Verified Customer | Posted 07/19/2019
    Fresh embedding sections - fix in ice-cold methanol 10 mins Blocking - 10% serum +1%BSA Primary - 5 ug/mL - overnight incubation Secondary - 1.5 hour incubation (Invitrogen A-11012, 2 ug/mL) mounting using Vector Vectashield with DAPI
    MDR1/ABCB1 Antibody (SN06-42) NBP2-67667

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Protocols

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