MEK1 [p Thr386] Antibody - Azide Free

Name: MEK1 [p Thr386] Antibody - Azide Free
Brand: Novus Biologicals
SKU: NB500-144
Price: 389 USD
Availability: InStock

Novus Biologicals | Catalog # NB500-144

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Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas
Related Blogs

Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Bovine (100%), Canine (100%), Chicken (100%), Mouse (100%), Primate (100%), Xenopus (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free

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Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr386 conjugated to KLH. Accession # Q02750

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 26446942)

Modification

p Thr386

Specificity

Specific for endogenous levels of the ~45 kDa MEK1 protein phosphorylated at Thr386. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MEK1 [p Thr386] Antibody - Azide Free

Western Blot: MEK1 [p Thr386] Antibody [NB500-144]

Western Blot: MEK1 [p Thr386] Antibody [NB500-144] - Analysis of recombinant WT and mutant MEK 1 immunolabeled with the Anti Thr386 MEK1 antibody. Lanes 1 and 2 are WT MEK 1 and Lanes 3 and 4 are mutant MEK 1 (T386A). MAP kinase was coexpressed in the samples run in Lanes 2 and 4.

Applications for MEK1 [p Thr386] Antibody - Azide Free

Application

Recommended Usage

Simple Western

1:50

Western Blot

1:1000

Application Notes

NB 500-144 can be used in Dot blots and Western blot where a band is seen at ~45 kDa, representing MEK 1. The investigator should determine the optimal dilution for a specific application.

See Simple Western Antibody Database for Simple Western validation: tested in HeLa + EGF; separated by size, antibody dilution of 1:50.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MEK1

MEK 1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993; Chong et al., 2003). This pathway also plays a key role in synaptic plasticity in brain (Adams and Sweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991; Seger et al., 1991; Crews et al., 1992) Conversely there also appears to be a feedback phosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylated by MAP kinase are Thr292 and Thr386 (Mansour et al., 1994).

Long Name

Mitogen-activated Protein Kinase Kinase 1

Alternate Names

MAP2K1, MKK1

Entrez Gene IDs

5604 (Human)

Gene Symbol

MAP2K1

UniProt

Q02750

Additional MEK1 Products

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Product Specific Notices for MEK1 [p Thr386] Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.