MEK1 [p Ser218, p Ser222] Antibody

Name: MEK1 [p Ser218, p Ser222] Antibody
Brand: Novus Biologicals
SKU: NBP2-24542
Price: 189 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-24542

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Canine, Chicken, Drosophila, Primate

Predicted:

Chimpanzee (100%), Orangutan (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

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Product Specifications

Immunogen

A portion of amino acids 200-250, containing phospho serine residues at positions 218 and 222, of human MEK1 was used as the immunogen.

Reactivity Notes

The amino acid sequence used as immunogen for this RXFP1 antibody is 83% homologous in Equine and Feline.

Modification

p Ser218, p Ser222

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MEK1 [p Ser218, p Ser222] Antibody

Western Blot: MEK1 [p Ser218, p Ser222] Antibody [NBP2-24542]

Western Blot: MEK1 Antibody [NBP2-24542] - Analysis of MEK1/MEK2 phosphorylation in A375 whole cell lysate. Lane A shows phospho MEK1/MEK2 (upper band), and the weakly detected nonphosphorylated form (lower band), using MEK1 phospho antibody at 3 ug/ml. Lane B shows the nonphosphorylated form of MEK1/2 using this antibody.

Applications for MEK1 [p Ser218, p Ser222] Antibody

Application

Recommended Usage

Western Blot

5 ug/ml

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MEK1

MEK 1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993; Chong et al., 2003). This pathway also plays a key role in synaptic plasticity in brain (Adams and Sweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991; Seger et al., 1991; Crews et al., 1992) Conversely there also appears to be a feedback phosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylated by MAP kinase are Thr292 and Thr386 (Mansour et al., 1994).

Long Name

Mitogen-activated Protein Kinase Kinase 1

Alternate Names

MAP2K1, MKK1

Gene Symbol

MAP2K1

Additional MEK1 Products

Product Documents for MEK1 [p Ser218, p Ser222] Antibody

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Certificate of Analysis

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Product Specific Notices for MEK1 [p Ser218, p Ser222] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.