Mesothelin Antibody (MB-G10) - BSA Free
Novus Biologicals | Catalog # NB110-85538
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Description
Store antibody at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for Mesothelin Antibody (MB-G10) - BSA Free
Western Blot: Mesothelin Antibody (MB-G10) [NB110-85538]
Western Blot: Mesothelin Antibody (MB-G10) [NB110-85538] - Western blotting using Mesothelin antibody (MB-G10). Load: Mesothelin-Fc (lane 1, 100 ng; lane 2, 25 ng; lane 3, 6 ng; lane 4, 2 ng; and lane 5, 0.4 ng) and CD25-Fc (lane 6, 50 ng)Primary antibody: anti-Mesothelin at 1mg/ml. Secondary Antibody: ALP goat anti-mouse IgG and BCIP/NBT substrate.Immunohistochemistry: Mesothelin Antibody (MB-G10) [NB110-85538]
Immunohistochemistry: Mesothelin Antibody (MB-G10) [NB110-85538] - Immunohistochemistry using Mesothelin antibody (MB-G10) to react with two epitopes on Mesothelin in PEFF human mesothelioma tissue sections treated by antigen retrieval methods. Anti-Mesothelin primary antibodies were used at 10 ug/mL to label these sections as follows: C, MAb MB; and D, MAb MN followed by goat anti-mouse IgG conjugated to Equineradish peroxidase at 25 ug/mL in 1% BSA/PBS for 30 minutes. (magnification, x200; bar, 50 um). Reprinted with permission from Clin.Cancer Res. 11(16):5840-6.SDS-PAGE: Mesothelin Antibody (MB-G10) [NB110-85538]
SDS-Page: Mesothelin Antibody (MB-G10) [NB110-85538] - SDS-PAGE of Mesothelin antibody (MB-G10). Lane 1: Non-Reduced Mesothelin antibody (MB-G10). Lane M: 3 uL OPAL Pre-stained Marker. Lane 2: Reduced Mesothelin antibody (MB-G10). Load: 1 ug per lane. Predicted/Observed size: Non-reduced at 160 kDa; Reduced at 55, 25 kDa.Mesothelin Antibody (MB-G10)
Immunohistochemistry using anti-mesothelin antibody to react with two epitopes on mesothelin in PEFF human mesothelioma tissue sections treated by antigen retrieval methods. Anti-mesothelin primary antibodies were used at 10 ug/mL to label these sections as follows: C, MAb MB; and D, MAb MN followed by goat anti-mouse IgG conjugated to horseradish peroxidase at 25 ug/mL in 1% BSA/PBS for 30 minutes. (magnification, x200; bar, 50 um). Reprinted with permission from Clin.Cancer Res. 11(16):5840-6.Immunohistochemistry: Mesothelin Antibody (MB-G10) - BSA Free [NB110-85538] -
Epicardial slices structure analysis. (a) Tissue architecture evaluation of epicardial slices performed using hematoxylin and eosin coloration. Scale bar, 500 μm. (b–e) Immunohistochemical staining and confocal microscopy of epicardial slices. (b) Cardiomyocytes are stained with alpha -sarcomeric actin and the intercalated disk is stained with connexin-43 antibody. (c) Epicardial cells highlighted with WT1 and mesenchymal derived cells stained with MSLN. (d) Microvasculature is stained with alpha - smooth muscle actin and the endothelial marker CD31. (e) Mesenchymal cells are stained with vimentin. Nuclei are labeled with DAPI. Scale bars, 50 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35924218), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for Mesothelin Antibody (MB-G10) - BSA Free
ELISA
Immunohistochemistry
Immunohistochemistry-Paraffin
Western Blot
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Background: Mesothelin
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Product Documents for Mesothelin Antibody (MB-G10) - BSA Free
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Product Specific Notices for Mesothelin Antibody (MB-G10) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars