MHC Class I Antibody (OX18) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80848

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, ELISA, Block/Neutralize, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OX18

Format

Azide and BSA Free
View all MHC Class I Primary Antibodies »

Product Specifications

Immunogen

Rat spleen glycoproteins

Localization

Cell Membrane; Type I membrane protein

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for MHC Class I Antibody (OX18) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: MHC Class I Antibody (OX18) - Azide and BSA Free [NBP2-80848]

Immunocytochemistry/ Immunofluorescence: MHC Class I Antibody (OX18) - Azide and BSA Free [NBP2-80848]

Immunocytochemistry/Immunofluorescence: MHC Class I Antibody (OX18) - Azide and BSA Free [NBP2-80848] - PC12 cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-MHC Class I Antibody (OX18) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.
Immunohistochemistry: MHC Class I Antibody (OX18) - Azide and BSA Free [NBP2-80848]

Immunohistochemistry: MHC Class I Antibody (OX18) - Azide and BSA Free [NBP2-80848]

Immunohistochemistry: MHC Class I Antibody (OX18) - Azide and BSA Free [NBP2-80848] - Analysis of FFPE rat brain cerebellum using MHC Class I (OK18) antibody at 1:200 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous p
Flow Cytometry: MHC Class I Antibody (OX18) - Azide and BSA Free [NBP2-80848]

Flow Cytometry: MHC Class I Antibody (OX18) - Azide and BSA Free [NBP2-80848]

Flow Cytometry: MHC Class I Antibody (OX18) - Azide and BSA Free [NBP2-80848] - Analysis using the FITC conjugate of NB120-6405. Staining of rat spleen cells with mouse anti-rat RT1-A (OX18).
Immunocytochemistry/ Immunofluorescence: MHC Class I Antibody (OX18) - Azide and BSA Free [NBP2-80848]

Immunocytochemistry/ Immunofluorescence: MHC Class I Antibody (OX18) - Azide and BSA Free [NBP2-80848]

Immunocytochemistry/Immunofluorescence: MHC Class I Antibody (OX18) - Azide and BSA Free [NBP2-80848] - Major histocompatibility complex (MHC) I and II as well as Transporter associated with antigen presentation II (TAPII) were analyzed, using immunocytochemistry on rat Schwann cells (SCs). Corresponding merges are shown in the bottom rows. Treatment of SCs with IL-17 was performed at concentrations of 0.5 and 50 ng/mL. Graphs to the right show densitometry quantification. SCs showed expression of MHCI > TAPII > MHCII, which increased after IL-17 treatment. MHCI was mainly detected in the cytoplasm and the expression increased in a dose-dependent manner after IL-17 treatment, significant for 0.5 ng/mL and 50 ng/mL (**P <=0.01). Image collected and cropped by CiteAb from the following publication (https://jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094…), licensed under a CC-BY license. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: MHC Class I Antibody (OX18) - Azide and BSA Free [NBP2-80848]

Immunocytochemistry/ Immunofluorescence: MHC Class I Antibody (OX18) - Azide and BSA Free [NBP2-80848]

Immunocytochemistry/Immunofluorescence: MHC Class I Antibody (OX18) - Azide and BSA Free [NBP2-80848] - PC-12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-MHC Class I (OX18) NB120-6405 at a 1:100 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.

Applications for MHC Class I Antibody (OX18) - Azide and BSA Free

Application
Recommended Usage

Block/Neutralize

reported in scientific literature (PMID 1698855)

ELISA

1:100 - 1:2000. Use reported in scientific literature (PMID 2783579)

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:10-1:500. Use reported in scientific literature (PMID 24678820)

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500
Application Notes
The epitope recognized by this anti-rat RT1-A antibody is reported to be sensitive to formaldehyde fixation and tissue processing. The use of acetone fixation for frozen sections is recommended. This antibody is CyTOF ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MHC Class I

MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.

Alternate Names

DKFZp686P19218, EA1.2, EA2.1, HLA class I histocompatibility antigen, alpha chain E, HLA class I histocompatibility antigen, E alpha chain, HLA-6.2, HLAE, lymphocyte antigen, major histocompatibility complex, class I, E, MHC, MHC class I antigen E, MHC HLA-E alpha-1, MHC HLA-E alpha-2.1, QA1

Gene Symbol

HLA-E

Additional MHC Class I Products

Product Documents for MHC Class I Antibody (OX18) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for MHC Class I Antibody (OX18) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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