MIF Antibody - Azide Free

Novus Biologicals | Catalog # NBP1-32192

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (91%), Porcine (94%), Rhesus Macaque (100%), Sheep (91%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free
View all MIF Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human MIF. The exact sequence is proprietary.

Localization

Secreted, Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit MIF Antibody - Azide Free (NBP1-32192) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for MIF Antibody - Azide Free

Western Blot: MIF Antibody [NBP1-32192]

Western Blot: MIF Antibody [NBP1-32192]

Western Blot: MIF Antibody [NBP1-32192] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with MIF antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Immunocytochemistry/ Immunofluorescence: MIF Antibody [NBP1-32192]

Immunocytochemistry/ Immunofluorescence: MIF Antibody [NBP1-32192]

Immunocytochemistry/Immunofluorescence: MIF Antibody [NBP1-32192] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: MIF Antibody [NBP1-32192]

Immunohistochemistry-Paraffin: MIF Antibody [NBP1-32192]

Immunohistochemistry-Paraffin: MIF Antibody [NBP1-32192] - Paraffin-embedded mouse brain. MIF antibody [N1C3] diluted at 1:500.
Western Blot: MIF Antibody [NBP1-32192]

Western Blot: MIF Antibody [NBP1-32192]

Western Blot: MIF Antibody [NBP1-32192] - Sample (30 ug of whole cell lysate)A: Molt-4 15% SDS PAGE, antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: MIF Antibody [NBP1-32192]

Immunohistochemistry-Paraffin: MIF Antibody [NBP1-32192]

Immunohistochemistry-Paraffin: MIF Antibody [NBP1-32192] - : Paraffin-embedded rat brain. MIF antibody [N1C3] diluted at 1:500.
ELISA: MIF Antibody [NBP1-32192]

ELISA: MIF Antibody [NBP1-32192]

ELISA: MIF Antibody [NBP1-32192] - ELISA detection of MIF [N1C3] capture at a concentration of 5 ug/mL and MIF C-term antibody for detection at a concentration of 1.5 ug/mL.
MIF Antibody - Azide Free

Western Blot: MIF Antibody [NBP1-32192] -

Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with MIF antibody [N1C3] (NBP1-32192) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
MIF Antibody - Azide Free

Western Blot: MIF Antibody [NBP1-32192] -

Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with MIF antibody [N1C3] (NBP1-32192) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for MIF Antibody - Azide Free

Application
Recommended Usage

ELISA

1:1000-1:10000

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Sandwich ELISA

Assay dependent

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Format

Azide Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MIF

Macrophages comprise of many forms of mononuclear phagocytes found in tissues. Mononuclear phagocytes arise from hematopoietic stem cells in the bone marrow. After passing through the monoblast and promonocyte states of the monocyte stage, they enter the blood, where they circulate for about 40 hours. They then enter tissues and increase in size, phagocytic activity, and lysosomal enzyme content becoming macrophages. Among the functions of macrophages are nonspecific phagocytosis and pinocytosis, specific phagocytosis of opsonized microorganisms mediated by Fc receptors and complement receptors, killing of ingested microorganisms, digestion and presentation of antigens to T and B lymphocytes, and secretion of a large number of diverse products, including many enzymes including lysozyme and collagenases, several complement components and coagulation factors, some prostaglandins and leukotrienes, and many regulatory molecules (Interferon, Interleukin 1). Among cells that are now recognized as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells, and Langerhans cells (in normal tissues) and epithelioid cells and Langerhans-type and foreign-body-type multinucleated giant cells (in inflamed tissues).

Long Name

Macrophage Migration Inhibitory Factor

Alternate Names

EC 5.3.2.1, EC 5.3.3.12, GIFmacrophage migration inhibitory factor, GLIF, Glycosylation-inhibiting factor, L-dopachrome isomerase, L-dopachrome tautomerase, macrophage migration inhibitory factor (glycosylation-inhibiting factor), MMIF, Phenylpyruvate tautomerase

Gene Symbol

MIF

Additional MIF Products

Product Documents for MIF Antibody - Azide Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS
Download SDS

Product Specific Notices for MIF Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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