MimEX Tissue Processing Kit

Kit Summary

Reagents for the processing of tissue samples for isolation of adult epithelial stem cells.

Benefits of Isolating Adult Stem Cells from Epithelial Tissue

Adult stem cell populations are found in most tissues of the human body and provide a long-lived source of self-renewing cells for both tissue homeostasis under normal conditions as well as for regeneration during disease or injury. In the adult, tissue specific stem cells can be either unipotent or multipotent. Tissue-specific adult stem cells exhibit an epigenetic memory conferring tissue specificity and regional identity. Moreover, adult stem cells may hold the keys to understanding aging, disease progression, and the development and spread of cancer. The MimEX™ Tissue Model System allow for the expansion and differentiation of ground-state, adult stem cell populations. These cells create sustainable and accessible 3-dimensional tissues in vitro and provide a valuable tool for biomarker discovery, disease modeling, drug screening*, and developmental biology studies.

The MimEX™ Tissue Processing Kit contains reagents and supplies for the collection and dissociation of adult epithelial tissue samples for the isolation of adult epithelial stem cells. The quantity of each component is sufficient to dissociate 1 tissue sample for stem cell isolation, up to 3 cm in size. Adult epithelial stem cells isolated using the tissue processing kit maintain local tissue regional identity, are genomically stable, and also preserve diseased tissue state.

This kit contains the following reagents to isolate epithelial stem cells from biopsy tissue:

• 30 mL of MimEX™ Tissue Collection Media
• 250 mL of MimEX™ Stem Cell Isolation Media
• 10 mL of MimEX™ Dissociation Solution
• 2 Scalpels

Precaution

When handling biohazardous materials such as human cells, safe laboratory procedures should be followed and protective clothing should be worn.

Limitations

• FOR LABORATORY RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
• The safety and efficacy of this product in diagnostic or other clinical uses has not been established.
• These reagents should not be used beyond the expiration date indicated on the label.

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Optimal Density of Adult Stem Cells Following Tissue Biopsy Dissociation and Digestion. Adult human intestinal epithelial tissue was digested and dissociated using the protocol described in the MimEX™ Tissue Collection Kit. A complete dissociation should yield mostly clusters of 2-5 cells. Some single cells and few large cell clusters may also be observed.

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Intestinal Epithelium after Dissection from Surrounding Stroma and Adipose Tissue. Human intestinal tissue was dissected using the protocol provided in the MimEX™ Tissue Collection Kit. Low (A), medium (B), and high magnification (C) images of intestinal crypts (arrowheads).

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Removal of Human Fibroblasts from Stem Cell Cultures Following Biopsy Dissection. (A, B) Images on the top show human fibroblast overgrowth (arrows) onto the top of an adult epithelial stem cell colony after 2-3 passages in culture. (C, D) Images on the bottom shows adult stem cell colonies after human fibroblast removal using MagCellect™ Plus Human EpCAM+ Cell Isolation Kit (R&D Systems®; Catalog # MAGH121).

* Portions of MimEX Tissue Model Systems are covered by a license to intellectual property owned by, or licensed to, Multiclonal Therapeutics, Inc. The purchase of this product conveys to the buyer the limited, non-exclusive, non-transferable right under these intellectual property rights to use this product for research purposes only. The purchase of this product does not include any right to use this product or components of this product for resale, for drug discovery intended for commercial gain, for the generation of cells or tissues intended for transplantation in human patients, or for any other commercial purposes, including without limitation, provision of services to a third party, generation of commercial databases, or as a diagnostic or therapeutic. Multiclonal Therapeutics, Inc., reserves all other rights under these rights. For information on purchasing a license to the intellectual property for uses other than in conjunction with this product or to use this product for purposes other than research, please contact Multiclonal Therapeutics, Inc. at licensing@multiclonaltherpaeutics.com.

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