This protocol video describes the MimEX™ Cell Plating Procedure. This procedure is important when using the MimEX™ Tissue Model System from R&D Systems. First, the MimEX™ Cell Plating Procedure is used to maintain a uniform irradiated fibroblast monolayer for the rapid clonal expansion and efficient differentiation of tissue-specific adult epithelial stem cells. It is also used to optimize the seeding distribution of adult stem cells on top of the fibroblasts monolayer. This distribution helps to maintain the health and rapid proliferation rate of adult epithelial stem cells using MimEX™ Tissue Model Systems.
Briefly, the cell plating procedure consists of three shakes of the plate backward and forward and three shakes of the plate side-to-side. This procedure is repeated three times before letting the cells settle into the well.