Mitofusin 1 Antibody (11E9-1H12) - BSA Free

Novus Biologicals | Catalog # NBP1-71775

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat, Gerbil

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 11E9-1H12

Format

BSA Free
View all Mitofusin 1 Primary Antibodies »

Product Specifications

Immunogen

Mouse Mitofusin 1 [Swiss-Prot: Q811U4]

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 23658809). Human reactivity reported in scientific literature (PMID: 24710686, 24928681).

Localization

Mitochondrion outer membrane; Multi-pass membrane protein

Marker

Mitochondrial Fusion Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Mitofusin 1 Antibody (11E9-1H12) - BSA Free

Western Blot: Mitofusin 1 Antibody (11E9-1H12)BSA Free [NBP1-71775]

Western Blot: Mitofusin 1 Antibody (11E9-1H12)BSA Free [NBP1-71775]

Mitofusin-1-Antibody-11E9-1H12-Western-Blot-NBP1-71775-img0014.jpg
Immunocytochemistry/ Immunofluorescence: Mitofusin 1 Antibody (11E9-1H12) - BSA Free [NBP1-71775]

Immunocytochemistry/ Immunofluorescence: Mitofusin 1 Antibody (11E9-1H12) - BSA Free [NBP1-71775]

Immunocytochemistry/Immunofluorescence: Mitofusin 1 Antibody (11E9-1H12) [NBP1-71775] - Antibody was tested in 3T3 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry-Paraffin: Mitofusin 1 Antibody (11E9-1H12) - BSA Free [NBP1-71775]

Immunohistochemistry-Paraffin: Mitofusin 1 Antibody (11E9-1H12) - BSA Free [NBP1-71775]

Immunohistochemistry-Paraffin: Mitofusin 1 Antibody (11E9-1H12) [NBP1-71775] - Analysis of Mitofusin-1 in mouse liver.
Western Blot: Mitofusin 1 Antibody (11E9-1H12)BSA Free [NBP1-71775]

Western Blot: Mitofusin 1 Antibody (11E9-1H12)BSA Free [NBP1-71775]

Western Blot: Mitofusin 1 Antibody (11E9-1H12) [NBP1-71775] - Analysis of Mitofusin-1 expression in (1) MEF wild-type and (2) MEF Mitofusin-1-null whole cell lysates using NBP1-71775.
Mitofusin 1 Antibody (11E9-1H12) - BSA Free

Western Blot: Mitofusin 1 Antibody (11E9-1H12) - BSA Free [NBP1-71775] -

Western Blot: Mitofusin 1 Antibody (11E9-1H12) - BSA Free [NBP1-71775] - Effect of AIF overexpression on mitochondrial fission & fusion in the cortex.a Representative immunoblots of mitochondrial fission proteins (P-DRP1 & FIS1) in the mitochondrial fraction from cortical tissue of P9 WT & AIF Tg mice under physiological conditions. Quantification of P-DRP1 & FIS1 did not show a significant difference between WT & AIF Tg mice (n = 6/group). b Representative immunoblots of mitochondrial fusion proteins (OPA1 & MFN1) in the mitochondrial fraction from cortical tissue of P9 WT & AIF Tg mice under physiological conditions. Quantification of OPA1 & MFN1 did not show any significant difference between WT & AIF Tg mice (n = 6/group). c Immunoblotting of P-DRP1and FIS1 in the mitochondrial fraction from the cortical tissue of the CL & IL hemispheres in WT & AIF Tg mice at 24 h after HI. Quantification of P-DRP1 & FIS1 did not show any significant differences in the CL & IL hemispheres between WT & AIF Tg mice (n = 6/group). d Immunoblotting of OPA1 & MFN1 in the mitochondrial fraction from cortical tissue of the CL & IL hemispheres at 24 h after HI in WT & AIF Tg mice. The expression of MFN1 was significantly decreased at 24 h post-HI both in WT & AIF Tg mice, while the short form (82 kDa) & cleavage band of OPA1 were significantly increased at 24 h post-HI in both the WT & AIF Tg mice. Quantification of OPA1 & MFN1 did not show any significant differences in the CL & IL hemispheres between WT & AIF Tg mice (n = 6/group). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32001673), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Mitofusin 1 Antibody (11E9-1H12) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Western Blot

1:500-1:1000
Application Notes
In Western blot, a band can be seen at ~80 kDa.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Mitofusin 1

Mitochondria are dynamic organelles that continuously divide and fuse, and their dynamics/distribution is critical for their role in bioenergetics and cell survival. Mitochondrial fusion process is mediated by mitofusin 1 (MFN1) and mitofusin 2 (MFN2) proteins in the outer membrane as well as OPA1 in the inner mitochondrial membrane. MFN1 is an essential transmembrane GTPase critical to mitochondrial fusion; acts independently of the cytoskeleton, and its overexpression induces formation of mitochondrial networks. MFN1 forms homo-/-heteromultimers (with MFN2), and the multimerization has been proposed to be essential for mitochondrion fusion. MFN1 interacts with VAT1 and is ubiquitinated by MARCH5. A mutation in the GTPase domain disrupts the mitochondrial fusion activity of MFN1/MFN2 and loss of MFN1/2 leads to mitochondrial dysfunction mediated embryonic lethality in mice. MFN proteins have been reported to regulate mitochondrial membrane potential, while silencing of MFN1/2 resulte in mitochondrial fragmentation and increased apoptotic sensitivity.

Alternate Names

hfzo1, hfzo2, MFN1

Entrez Gene IDs

55669 (Human)

Gene Symbol

MFN1

Additional Mitofusin 1 Products

Product Documents for Mitofusin 1 Antibody (11E9-1H12) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Mitofusin 1 Antibody (11E9-1H12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Bcl-2 Proteins and Regulators

Citations for Mitofusin 1 Antibody (11E9-1H12) - BSA Free

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Protocols

View specific protocols for Mitofusin 1 Antibody (11E9-1H12) - BSA Free (NBP1-71775):


Immunocytochemistry Protocol

Culture cells to appropriate density in 35 mm culture dishes or 6-well plates.

1. Remove culture medium and add 10% formalin to the dish. Fix at room temperature for 30 minutes.
2. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
3. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
4. To block nonspecific antibody binding incubate in 10% normal goat serum from 1 hour to overnight at room temperature.
5. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
6. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
7. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
8. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
9. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.

*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.

Western Blot Protocol

1. Perform SDS-PAGE on samples to be analyzed, loading 40 ug of total protein per lane.
2. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
3. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
4. Rinse the blot.
5. Block the membrane using standard blocking buffer for at least 1 hour.
6. Wash the membrane in wash buffer three times for 10 minutes each.
7. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
8. Wash the membrane in wash buffer three times for 10 minutes each.
9. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
10. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturers instructions.

*Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2%.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Mitofusin 1 Antibody (11E9-1H12) - BSA Free

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  • Q: Could you please tell me if you'd offer the mitofusin 1 antibody Cat# NBP1-71775 for using it with rat tissue? If not, could you please offer one that works with rats?

    A:

    NBP1-71775 has been validated for use in human and mouse only. Rat is not one of the listed species and we cannot guarantee that it will work in rat. Unfortunately, we currently do not have a Mitofusin-1 antibody that has been tested and validated for use in rat. If you'd be interested in testing one of our antibodies, we can recommend our Innovator's Reward program. Our Innovator's Reward is offered to reward researchers for testing new species and applications with our products. If testing on this antibody is performed on an untested species and/or application, and the results are shared, then the antibody is eligible for the nnovator's Reward. Under this program, Novus will provide you a credit of equal or lesser value on a future product of equal value in exchange for your new data in the form of an online review. The great thing is you are eligible even if the antibody doesn't work because the data is still useful to us. (Note: Rat was added as a species on the datasheet from a researcher who published using rat in May 2013 - please see PMID 23658809 for details).

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