MKP-3/DUSP6 Antibody (3G2) - Azide and BSA Free

Novus Biologicals | Catalog # H00001848-M01

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3G2

Format

Azide and BSA Free
View all MKP-3 Primary Antibodies »

Product Specifications

Immunogen

DUSP6 (AAH03143, 1 a.a. ~ 381 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MIDTLRPVPFASEMAISKTVAWLNEQLELGNERLLLMDCRPQELYESSHIESAINVAIPGIMLRRLQKGNLPVRALFTRGEDRDRFTRRCGTDTVVLYDESSSDWNENTGGESLLGLLLKKLKDEGCRAFYLEGGFSKFQAEFSLHCETNLDGSCSSSSPPLPVLGLGGLRISSDSSSDIESDLDRDPNSATDSDGSPLSNSQPSFPVEILPFLYLGCAKDSTNLDVLEEFGIKYILNVTPNLPNLFENAGEFKYKQIPISDHWSQNLSQFFPEAISFIDEARGKNCGVLVHCLAGISRSVTVTVAYLMQKLNLSMNDAYDIVKMKKSNISPNFNFMGQLLDFERTLGLSSPCDNRVPAQQLYFTTPSNQNVYQVDSLQST

Localization

Cytoplasmic

Specificity

DUSP6 - dual specificity phosphatase 6

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for MKP-3/DUSP6 Antibody (3G2) - Azide and BSA Free

Western Blot: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01]

Western Blot: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01]

Western Blot: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01] - Analysis of DUSP6 expression in transfected 293T cell line by DUSP6 monoclonal antibody (M01), clone 3G2.Lane 1: DUSP6 transfected lysate(42 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01]

Immunocytochemistry/ Immunofluorescence: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01]

Immunocytochemistry/Immunofluorescence: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01] - Analysis of monoclonal antibody to DUSP6 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01]

Immunohistochemistry-Paraffin: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01]

Immunohistochemistry-Paraffin: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01] - Analysis of monoclonal antibody to DUSP6 on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 3 ug/ml.
Western Blot: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01]

Western Blot: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01]

Western Blot: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01] - DUSP6 monoclonal antibody (M01), clone 3G2 Western Blot analysis of DUSP6 expression in Jurkat ( Cat # L017V1 ).
Western Blot: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01]

Western Blot: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01]

Western Blot: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01] - DUSP6 monoclonal antibody (M01), clone 3G2. Western Blot analysis of DUSP6 expression in human placenta.
Western Blot: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01]

Western Blot: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01]

Western Blot: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01] - Analysis of DUSP6 over-expressed 293 cell line, cotransfected with DUSP6 Validated Chimera RNAi ( Cat # H00001848-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with DUSP6 monoclonal antibody (M01), clone 3G2 (Cat # H00001848-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
ELISA: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01]

ELISA: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01]

ELISA: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01] - Detection limit for recombinant GST tagged DUSP6 is approximately 1ng/ml as a capture antibody.

Applications for MKP-3/DUSP6 Antibody (3G2) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against recominant protein, tissue lysate, transfected lysate and cell lysate for WB. It has been used for IF, IHC-P, RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MKP-3

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK2, is expressed in a variety of tissues with the highest levels in heart and pancreas, and unlike most other members of this family, is localized in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene.

Long Name

MAP Kinase Phosphatase 3

Alternate Names

DUSP6, MKP3, PYST1

Entrez Gene IDs

1848 (Human)

Gene Symbol

DUSP6

OMIM

602748 (Human)

UniProt

AAH03143 (Human)

Additional MKP-3 Products

Product Documents for MKP-3/DUSP6 Antibody (3G2) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for MKP-3/DUSP6 Antibody (3G2) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MKP-3/DUSP6 Antibody (3G2) - Azide and BSA Free

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Protocols

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FAQs

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