MLH3 Antibody (CL14084) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-43849

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL14084

Format

Azide and BSA Free
View all MLH3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of Q9UHC1, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for MLH3 Antibody (CL14084) - Azide and BSA Free

MLH3 Antibody (CL14084) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: MLH3 Antibody [NBP3-43849]

Immunocytochemistry/ Immunofluorescence: MLH3 Antibody [NBP3-43849]

Staining in A-431 cell line with Anti-MLH3 monoclonal antibody).
MLH3 Antibody (CL14084) - Azide and BSA Free Immunohistochemistry-Paraffin: MLH3 Antibody [NBP3-43849]

Immunohistochemistry-Paraffin: MLH3 Antibody [NBP3-43849]

Staining of human breast cancer (ductal carcinoma) shows strong nuclear positivity in tumor cells.
MLH3 Antibody (CL14084) - Azide and BSA Free Immunohistochemistry-Paraffin: MLH3 Antibody [NBP3-43849]

Immunohistochemistry-Paraffin: MLH3 Antibody [NBP3-43849]

Analysis in human endometrium and skeletal muscle tissues using NBP3-43849 antibody. Corresponding MLH3 RNA-seq data are presented for the same tissues.
MLH3 Antibody (CL14084) - Azide and BSA Free Immunohistochemistry-Paraffin: MLH3 Antibody [NBP3-43849]

Immunohistochemistry-Paraffin: MLH3 Antibody [NBP3-43849]

Staining of human fallopian tube shows moderate nuclear positivity in glandular cells.
MLH3 Antibody (CL14084) - Azide and BSA Free Immunohistochemistry-Paraffin: MLH3 Antibody [NBP3-43849]

Immunohistochemistry-Paraffin: MLH3 Antibody [NBP3-43849]

Staining of human endometrial cancer shows strong nuclear positivity in tumor cells.
MLH3 Antibody (CL14084) - Azide and BSA Free Immunohistochemistry-Paraffin: MLH3 Antibody [NBP3-43849]

Immunohistochemistry-Paraffin: MLH3 Antibody [NBP3-43849]

Staining of human endometrium shows strong nuclear positivity in glandular cells.
MLH3 Antibody (CL14084) - Azide and BSA Free Immunohistochemistry-Paraffin: MLH3 Antibody [NBP3-43849]

Immunohistochemistry-Paraffin: MLH3 Antibody [NBP3-43849]

Staining of human rectum shows moderate nuclear positivity in glandular cells.
MLH3 Antibody (CL14084) - Azide and BSA Free Immunohistochemistry-Paraffin: MLH3 Antibody [NBP3-43849]

Immunohistochemistry-Paraffin: MLH3 Antibody [NBP3-43849]

Staining of human placenta shows moderate to strong nuclear positivity in trophoblastic cells.
MLH3 Antibody (CL14084) - Azide and BSA Free Immunohistochemistry-Paraffin: MLH3 Antibody [NBP3-43849]

Immunohistochemistry-Paraffin: MLH3 Antibody [NBP3-43849]

Staining of human skeletal muscle shows no nuclear positivity in myocytes as expected.
MLH3 Antibody (CL14084) - Azide and BSA Free Immunohistochemistry-Paraffin: MLH3 Antibody [NBP3-43849]

Immunohistochemistry-Paraffin: MLH3 Antibody [NBP3-43849]

Staining of human ovarian cancer (high grade serous ovarian carcinoma) shows strong nuclear positivity in tumor cells.
MLH3 Antibody (CL14084) - Azide and BSA Free Western Blot: MLH3 Antibody [NBP3-43849]

Western Blot: MLH3 Antibody [NBP3-43849]

Analysis in human cell line SCLC-21H.

Applications for MLH3 Antibody (CL14084) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments, For Immunocytochemistry/ Immunofluorescence/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MLH3

MLH3 is a member of the MutL-homolog (MLH) family of DNA mismatch repair (MMR) genes. MLH genes are implicated in maintaining genomic integrity during DNA replication and after meiotic recombination. The protein encoded by this gene functions as a heterodimer with other family members. Somatic mutations in this gene frequently occur in tumors exhibiting microsatellite instability, and germline mutations have been linked to hereditary nonpolyposis colorectal cancer type 7 (HNPCC7). Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined. [provided by RefSeq]

Alternate Names

DNA mismatch repair protein Mlh3, HNPCC7, MGC138372, mutL (E. coli) homolog 3, mutL homolog 3 (E. coli), MutL protein homolog 3

Gene Symbol

MLH3

Additional MLH3 Products

Product Documents for MLH3 Antibody (CL14084) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for MLH3 Antibody (CL14084) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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