MMP-1 Antibody (6A5) - BSA Free

Novus Biologicals | Catalog # NBP2-22123

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 6A5

Format

BSA Free
View all MMP-1 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human MMP1 expressed in E. coli. [UniProt# P03956]

Reactivity Notes

Other species have not been tested.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MMP-1 Antibody (6A5) - BSA Free

Western Blot: MMP-1 Antibody (6A5)BSA Free [NBP2-22123]

Western Blot: MMP-1 Antibody (6A5)BSA Free [NBP2-22123]

Western Blot: MMP-1 Antibody (6A5) [NBP2-22123] - Western blot analysis using MMP1 mAb against HEK293 (1) and MMP1(AA: 24-213)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: MMP-1 Antibody (6A5) - BSA Free [NBP2-22123]

Immunocytochemistry/ Immunofluorescence: MMP-1 Antibody (6A5) - BSA Free [NBP2-22123]

Immunocytochemistry/Immunofluorescence: MMP-1 Antibody (6A5) [NBP2-22123] - Immunofluorescence analysis of Hela cells using MMP1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: MMP-1 Antibody (6A5) - BSA Free [NBP2-22123]

Immunohistochemistry-Paraffin: MMP-1 Antibody (6A5) - BSA Free [NBP2-22123]

Immunohistochemistry-Paraffin: MMP-1 Antibody (6A5) [NBP2-22123] - Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues (left) and human kidney cancer tissues (right) using MMP1 mouse mAb with DAB staining.
Flow Cytometry: MMP-1 Antibody (6A5) - BSA Free [NBP2-22123]

Flow Cytometry: MMP-1 Antibody (6A5) - BSA Free [NBP2-22123]

Flow Cytometry: MMP-1 Antibody (6A5) [NBP2-22123] - Flow cytometric analysis of Hela cells using MMP1 mouse mAb (green) and negative control (purple).
ELISA: MMP-1 Antibody (6A5) - BSA Free [NBP2-22123]

ELISA: MMP-1 Antibody (6A5) - BSA Free [NBP2-22123]

ELISA: MMP-1 Antibody (6A5) [NBP2-22123] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).

Applications for MMP-1 Antibody (6A5) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
This MMP1 (6A5) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry/Immunofluorescence, Flow Cytometry and ELISA.

Reviewed Applications

Read 1 review rated 4 using NBP2-22123 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MMP-1

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants.

Long Name

Matrix Metalloproteinase 1

Alternate Names

MMP1

Entrez Gene IDs

4312 (Human)

Gene Symbol

MMP1

UniProt

P03956

Additional MMP-1 Products

Product Documents for MMP-1 Antibody (6A5) - BSA Free

Certificate of Analysis

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Product Specific Notices for MMP-1 Antibody (6A5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MMP-1 Antibody (6A5) - BSA Free

Customer Reviews for MMP-1 Antibody (6A5) - BSA Free (1)

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  • MMP-1 Antibody (6A5) - BSA Free
    Name: Shinford Sun
    Application: Immunocytochemistry
    Sample Tested: fibroblasts, human fibroblast cells and mouse liver tissue, Sample Amount: 100 ug and Human lung cancer tissue samples
    Species: Human
    Verified Customer | Posted 08/11/2023
    We utilized various fibroblast markers to pinpoint the potential functional candidate for detecting active CAF signatures. MMP-1 exhibited dysregulation as a result of distinct treatments.
    MMP-1 Antibody (6A5) - BSA Free NBP2-22123

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