MMP-9 Antibody (5G3)

Novus Biologicals | Catalog # NBP2-22181

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

ELISA, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 5G3
View all MMP-9 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human MMP-9 expressed in E. Coli.

Localization

Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MMP-9 Antibody (5G3)

Western Blot: MMP-9 Antibody (5G3)BSA Free [NBP2-22181]

Western Blot: MMP-9 Antibody (5G3)BSA Free [NBP2-22181]

Western Blot: MMP-9 Antibody (5G3) [NBP2-22181] - Analysis using MMP9 mAb against human MMP9 (AA: 238-465) recombinant protein. (Expected MW is 50.6 kDa)
Immunocytochemistry/ Immunofluorescence: MMP-9 Antibody (5G3) - BSA Free [NBP2-22181]

Immunocytochemistry/ Immunofluorescence: MMP-9 Antibody (5G3) - BSA Free [NBP2-22181]

Immunocytochemistry/Immunofluorescence: MMP-9 Antibody (5G3) [NBP2-22181] - Analysis of NIH/3T3 cells using MMP9 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: MMP-9 Antibody (5G3) - BSA Free [NBP2-22181]

Immunohistochemistry-Paraffin: MMP-9 Antibody (5G3) - BSA Free [NBP2-22181]

Immunohistochemistry-Paraffin: MMP-9 Antibody (5G3) [NBP2-22181] - Analysis of tissues using MMP9 mouse mAb with DAB staining.
Flow Cytometry: MMP-9 Antibody (5G3) - BSA Free [NBP2-22181]

Flow Cytometry: MMP-9 Antibody (5G3) - BSA Free [NBP2-22181]

Flow Cytometry: MMP-9 Antibody (5G3) [NBP2-22181] - Analysis of Hela cells using MMP9 mouse mAb (blue) and negative control (red).
Western Blot: MMP-9 Antibody (5G3)BSA Free [NBP2-22181]

Western Blot: MMP-9 Antibody (5G3)BSA Free [NBP2-22181]

Western Blot: MMP-9 Antibody (5G3) [NBP2-22181] - Analysis using MMP9 mAb against HEK293 (1), MMP7-hIgGFc transfected HEK293 (2) cell lysate and MMP9-hIgGFc transfected HEK293 (3) cell lysate.
ELISA: MMP-9 Antibody (5G3) - BSA Free [NBP2-22181]

ELISA: MMP-9 Antibody (5G3) - BSA Free [NBP2-22181]

ELISA: MMP-9 Antibody (5G3) [NBP2-22181] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)

Applications for MMP-9 Antibody (5G3)

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MMP-9

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.

Long Name

Matrix Metalloproteinase 9

Alternate Names

CLG4B, Gelatinase B, GELB, MANDP2, MMP9

Gene Symbol

MMP9

Additional MMP-9 Products

Product Documents for MMP-9 Antibody (5G3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for MMP-9 Antibody (5G3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MMP-9 Antibody (5G3)

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for MMP-9 Antibody (5G3)

Showing  1 - 1 of 1 FAQ Showing All

  • Q:  I’m looking for a pair of antibodies to MMP-9 that can be used in a sandwich assay. Do you carry any? 

    A:

    We have 10 primary antibodies for MMP-9 that have been tested in ELISA, seen here.
    It looks like 2 have been tested for capture (please note the tested species for each of these), seen here.
    6 have been tested for detection, seen here.

Showing  1 - 1 of 1 FAQ Showing All
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