MMP-9 Antibody - BSA Free

Western Blot: MMP-9 Antibody - BSA Free [NBP2-41233] -

MLKL in macrophages affects myofibroblasts activities through PGE2. (A) The mRNA and protein expression of VEGF, MMP-9 in MLKL+/+ myofibroblasts treated with MLKL−/− M1ø CM or with PGE2 supplement. (B) The mRNA and protein expression of VEGF, MMP-9 in MLKL+/+ myofibroblasts treated with MLKL−/− M2ø CM or with PGE2 supplement. GAPDH was used as a loading control. Grayscale values were measured using ImageJ software. Littermate control mice were on a C57BL/6J genetic background were utilized in the experiments. Results were expressed as the mean +/- SD and were analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test (n = 4–6). *P < 0.05, **P < 0.01, ***P < 0.001. ns not significant. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40253554), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: MMP-9 Antibody - BSA Free [NBP2-41233] -

MLKL in macrophages affects myofibroblasts activities through PGE2. (A) The mRNA and protein expression of VEGF, MMP-9 in MLKL+/+ myofibroblasts treated with MLKL−/− M1ø CM or with PGE2 supplement. (B) The mRNA and protein expression of VEGF, MMP-9 in MLKL+/+ myofibroblasts treated with MLKL−/− M2ø CM or with PGE2 supplement. GAPDH was used as a loading control. Grayscale values were measured using ImageJ software. Littermate control mice were on a C57BL/6J genetic background were utilized in the experiments. Results were expressed as the mean +/- SD and were analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test (n = 4–6). *P < 0.05, **P < 0.01, ***P < 0.001. ns not significant. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40253554), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: MMP-9 Antibody - BSA Free [NBP2-41233] -

NLRP3 is involved in tissue growth related factors expression in wound site (A-D) Skin wound tissues from wild-type and NLRP3−/− mice were subjected to immunofluorescence staining for MMP-9 (green), ER alpha (red), EGF (green), VEGF (red), and nuclei (DAPI, blue) on days 3, 5, and 7 post-injury. The merged images represent the composite pictures of the target protein and nuclei. Immunofluorescence staining was visualized using fluorescence microscopy (Zeiss LSM 800 laser, ×200 magnification), and the mean fluorescence intensity was quantified (a.u., arbitrary units). The mRNA expression of MMP-9, ER alpha, EGF, and VEGF in wild-type and NLRP3−/− skin wound tissues was quantified using RT-PCR. GAPDH serves as internal reference. Results are expressed as mean +/- SD from multiple independent experiments and were analyzed using Student t test (n = 5). Statistical significance is indicated as follows: *P < 0.05, **P < 0.01, ***P < 0.001. Scale bar = 20 μm Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39881348), licensed under a CC-BY license. Not internally tested by Novus Biologicals.