Moesin Antibody (MSN/493)

Novus Biologicals | Catalog # NBP2-44580

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Rat (Negative)

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # MSN/493
View all Moesin Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human Moesin protein (Uniprot: P26038)

Reactivity Notes

Does not react with Rat.

Localization

Cytoplasmic & Cell Surface

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47917)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Moesin Antibody (MSN/493)

Knockout Validated: Moesin Antibody (MSN/493) [NBP2-44580]

Western Blot: Moesin Antibody (MSN/493) [NBP2-44580]

Western Blot: Moesin Antibody (MSN/493) [NBP2-44580] - Western blot using lysates from U20S parental cell line and Moesin-1 knockout U20S cell line (KO), collected in RIPA buffer. Nitrocellulose membrane was probed with Mouse Anti-Human/Mouse Moesin Monoclonal Antibody (Catalog # NBP2-44580) at a 1:1000 dilution O/N at 4C, followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody and ECL detection. A specific band was detected for Moesin-1 (as indicated) in the parental U20S cell line, but is not detectable in knockout U20S cell line. The Ponceau stained transfers of each blot are shown to confirm equal protein loading. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).
Immunocytochemistry/ Immunofluorescence: Moesin Antibody (MSN/493) [NBP2-44580]

Immunocytochemistry/ Immunofluorescence: Moesin Antibody (MSN/493) [NBP2-44580]

Immunocytochemistry/Immunofluorescence: Moesin Antibody (MSN/493) [NBP2-44580] - Immunofluorescent staining of paraformaldehyde-fixed HeLa cells. followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)
Western Blot: Moesin Antibody (MSN/493) [NBP2-44580]

Western Blot: Moesin Antibody (MSN/493) [NBP2-44580]

Western Blot: Moesin Antibody (MSN/493) [NBP2-44580] - Western Blot Analysis of PC3 cell lysate. Moesin antibody (MSN/493).
Immunohistochemistry-Paraffin: Moesin Antibody (MSN/493) [NBP2-44580]

Immunohistochemistry-Paraffin: Moesin Antibody (MSN/493) [NBP2-44580]

Immunohistochemistry-Paraffin: Moesin Antibody (MSN/493) [NBP2-44580] - Human Testicular Carcinoma stained with Moesin Monoclonal Antibody (MSN/493)
Flow Cytometry: Moesin Antibody (MSN/493) [NBP2-44580]

Flow Cytometry: Moesin Antibody (MSN/493) [NBP2-44580]

Flow Cytometry: Moesin Antibody (MSN/493) [NBP2-44580] - Flow Cytometric Analysis of paraformaldehyde-fixed K562 cells. Moesin Antibody (MSN/493) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red)
Western Blot: Moesin Antibody (MSN/493) [NBP2-44580]

Western Blot: Moesin Antibody (MSN/493) [NBP2-44580]

Western Blot: Moesin Antibody (MSN/493) [NBP2-44580] - Western Blot Analysis of human Jurkat cell lysate. Moesin Antibody (MSN/493).
Immunohistochemistry-Paraffin: Moesin Antibody (MSN/493) [NBP2-44580]

Immunohistochemistry-Paraffin: Moesin Antibody (MSN/493) [NBP2-44580]

Immunohistochemistry-Paraffin: Moesin Antibody (MSN/493) [NBP2-44580] - Human Melanoma stained with Moesin Monoclonal Antibody (MSN/493)
Immunohistochemistry-Paraffin: Moesin Antibody (MSN/493) [NBP2-44580]

Immunohistochemistry-Paraffin: Moesin Antibody (MSN/493) [NBP2-44580]

Immunohistochemistry-Paraffin: Moesin Antibody (MSN/493) [NBP2-44580] - Human Placenta stained with Moesin Monoclonal Antibody (MSN/493)
Knockout Validated: Moesin Antibody (MSN/493) [NBP2-44580]

Immunocytochemistry/ Immunofluorescence: Moesin Antibody (MSN/493) [NBP2-44580]

Immunocytochemistry/ Immunofluorescence: Moesin Antibody (MSN/493) [NBP2-44580] - Parental and MSN KO cells were labeled with a green or a far-red dye, respectively. Parental and KO cells were mixed and plated to a 1:1 ratio on coverslips. Cells were stained with anti-Moesin (NBP2-44580) antibodies and with the corresponding Alexa-fluor 555 coupled secondary antibody. Acquisition of the green (WT), red (antibody staining), and far-red (KO) channels was performed. Representative grayscale images of the red channel are shown, and WT and KO cells are outlined with a yellow and magenta dashed line, respectively. A schematic representation of the mosaic strategy used is shown on the right panel. Antibody dilution used: NBP2-44579 at 1/200. Bar = 10 um. Images and testing courtesy of YCharOS Inc. (ycharos.com).

Applications for Moesin Antibody (MSN/493)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

2-4 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Moesin

Moesin (membrane-organizing extension spike protein) has previously been characterized as a possible receptor protein for heparan sulfate and also as a cytoskeletal linker protein that stabilizes cell surface microvilli, filopodia and lamellipodia. Data indicate that moesin is identical to the 77-kDa band that copurifies with ezrin in its isolation from human placenta (1). Members of the ezrin-radixin-moesin (ERM) family of membrane-cytoskeletal linking proteins have NH2- and COOH-terminal domains that associate with the plasma membrane and the actin cytoskeleton, respectively (2). It has been demonstrated that ezrin-radixin-moesin proteins are rapidly inactivated after antigen recognition through a Vav1-Rac1 pathway. The resulting disanchoring of the cortical actin cytoskeleton from the plasma membrane decreased cellular rigidity, leading to more efficient T cell-antigen-presenting cell conjugate formation (3).

Alternate Names

Membrane-organizing extension spike protein, moesin

Gene Symbol

MSN

UniProt

P26038 (Human)

Additional Moesin Products

Product Documents for Moesin Antibody (MSN/493)

Certificate of Analysis

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Product Specific Notices for Moesin Antibody (MSN/493)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Moesin Antibody (MSN/493)

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  • Q: I am looking to use shRNA to inhibit Moesin expression. I have had people advise me that my initial MOI should be low as 'less is more' and 'a little goes a long way' in terms of siRNA. I was wondering if you could elaborate on this for me and explain why my initial MOI should be low.

    A: The reason for a low MOI is most likely because RNAi is a very strong and efficient technique. Wikipedia does a good job of explaining RNA interference. However, I would imagine that in a cell, there will be at most 1-2 copies of the gene mRNA present at any given time, unless you're dealing with a highly expressed protein such as Actin, where I would imagine silencing Actin would be lethal to the cell. I can imagine a few reasons to not use too much siRNA. First, it is expensive, so you don't want to waste it. Second, using too much would cause there to be a lot of non-translatable RNA present in the cell, which could trigger an immune response, as the presence of uncapped RNAs can indicate presence of a virus and one of the TLRs may respond to this.

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