Mouse 4-1BB/TNFRSF9/CD137 Antibody

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Product Details
Citations (4)
Supplemental Products
Reviews (2)

Mouse 4-1BB/TNFRSF9/CD137 Antibody Summary

Species Reactivity
Detects mouse 4-1BB in direct ELISAs and Western blots. In direct ELISAs, this antibody shows no cross-reactivity with recombinant mouse (rm) BAFF R, rmCD27, rmCD30, rmCD40, rmDR3, rmEDAR, rmFas, rmGITR, rmLT beta R, rmNGF R, rmOPG, rmRANK, rmTAJ, rmTNF RI, rmTNF RII, rh4-1BB, rhDR6, or rhHVEM.
Monoclonal Rat IgG2A Clone # 158321
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant mouse 4-1BB
Accession # P20334
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.


Recommended Concentration
Agonist Activity
Measured by its ability to co-stimulate IFN-gamma secretion by mouse splenic T cells in the presence of anti-CD3 [Saoulli, K. et al. (1998) J. Exp. Med. 187(11):1849; Cannons, J. et al. (2001) J. Immunol. 167:1313.].
The ED50 for this effect is typically 0.4 - 1.2 μg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 4-1BB/TNFRSF9/CD137

4-1BB is a type I transmembrane protein belonging to the TNF receptor superfamily. It has been designated TNFRSF9 and is also known as CD137. 4-1BB is expressed on T cells, NK cells, monocytes, neutrophils, dendritic cells, and eosinophils. The ligand for 4-1BB, 4-1BBL, is a TNF ligand superfamily member (TNFSF9) expressed on the surfaces of activated antigen presenting cells and T and B lymphoma cell lines. 4-1BB transduces a T cell costimulatory signal to promote survival, proliferation, cytokine production, and effector function. On dendritic cells, 4-1BB ligation enhances cytokine production and upregulates expression of B7-1 and B7-2 costimulatory molecules.

Entrez Gene IDs
3604 (Human); 21942 (Mouse); 500590 (Rat); 102127961 (Cynomolgus Monkey)
Alternate Names
4-1BB ligand receptor; 41BB; 4-1BB; CD137 antigen; CD137; CD137MGC2172; CDw137; FLJ43501; ILA; ILAhomolog of mouse 4-1BB; induced by lymphocyte activation (ILA); interleukin-activated receptor, homolog of mouse Ly63; receptor protein 4-1BB; T cell antigen ILA; T-cell antigen 4-1BB homolog; T-cell antigen ILA; TNFRSF9; tumor necrosis factor receptor superfamily member 9; tumor necrosis factor receptor superfamily, member 9

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Citations for Mouse 4-1BB/TNFRSF9/CD137 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Integrin &alphav&beta8 on T�cells suppresses anti-tumor immunity in multiple models and is a promising target for tumor immunotherapy
    Authors: E Dodagatta-, HY Ma, B Liang, J Li, DS Meyer, SY Chen, KH Sun, X Ren, B Zivak, MD Rosenblum, MB Headley, L Pinzas, NI Reed, JS Del Cid, BC Hann, S Yang, A Giddabasap, K Noorbehesh, B Yang, J Dal Porto, T Tsukui, K Niessen, A Atakilit, RJ Akhurst, D Sheppard
    Cell Reports, 2021;36(1):109309.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  2. Epitope and Fc-mediated Crosslinking, but not High Affinity, Are Critical for Antitumor Activity of CD137 Agonist Antibody with Reduced Liver Toxicity
    Authors: SK Ho, Z Xu, A Thakur, M Fox, SS Tan, E DiGiammari, L Zhou, M Sho, B Cairns, V Zhao, M Xiong, J Samayoa, CM Forsyth, DB Powers, DT Chao, D Hollenbaug, HM Alvarez, Y Akamatsu
    Mol. Cancer Ther., 2020;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Agonist Activity
  3. Combined triggering of dendritic cell receptors results in synergistic activation and potent cytotoxic immunity.
    Authors: Wells JW, Cowled CJ, Farzaneh F
    J. Immunol., 2008;181(5):3422-31.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  4. Excreted urinary mediators in an animal model of experimental immune nephritis with potential pathogenic significance.
    Authors: Wu T, Xie C, Bhaskarabhatla M, Yan M, Leone A, Chen SS, Zhou XJ, Putterman C, Mohan C
    Arthritis Rheum., 2007;56(3):949-59.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Control


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Reviews for Mouse 4-1BB/TNFRSF9/CD137 Antibody

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Mouse 4-1BB/TNFRSF9/CD137 Antibody
By Anonymous on 04/10/2019
Application: ELISA Sample Tested: M41BB antigen Species: Mouse

Mouse 4-1BB/TNFRSF9/CD137 Antibody
By Anonymous on 12/16/2018
Application: ELISA Sample Tested: Commercial Antigen Species: Mouse