Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Agonist Activity

Cited:

Neutralization, Control, In vivo assay, Agonist Activity

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 158321
View all 4-1BB/TNFRSF9/CD137 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse 4-1BB
Val24-Leu187
Accession # P20334

Specificity

Detects mouse 4-1BB in direct ELISAs and Western blots. In direct ELISAs, this antibody shows no cross-reactivity with recombinant mouse (rm) BAFF R, rmCD27, rmCD30, rmCD40, rmDR3, rmEDAR, rmFas, rmGITR, rmLT beta R, rmNGF R, rmOPG, rmRANK, rmTAJ, rmTNF RI, rmTNF RII, rh4-1BB, rhDR6, or rhHVEM.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications for Mouse 4‑1BB/TNFRSF9/CD137 Antibody

Application
Recommended Usage

Agonist Activity

Measured by its ability to co-stimulate IFN-gamma secretion by mouse splenic T cells in the presence of anti-CD3 [Saoulli, K. et al. (1998) J. Exp. Med. 187(11):1849; Cannons, J. et al. (2001) J. Immunol. 167:1313.].
The ED50 for this effect is typically 0.4 - 1.2 μg/mL.

Reviewed Applications

Read 4 reviews rated 5 using MAB9371 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: 4-1BB/TNFRSF9/CD137

4-1BB is a type I transmembrane protein belonging to the TNF receptor superfamily. It has been designated TNFRSF9 and is also known as CD137. 4-1BB is expressed on T cells, NK cells, monocytes, neutrophils, dendritic cells, and eosinophils. The ligand for 4-1BB, 4-1BBL, is a TNF ligand superfamily member (TNFSF9) expressed on the surfaces of activated antigen presenting cells and T and B lymphoma cell lines. 4-1BB transduces a T cell costimulatory signal to promote survival, proliferation, cytokine production, and effector function. On dendritic cells, 4-1BB ligation enhances cytokine production and upregulates expression of B7-1 and B7-2 costimulatory molecules.

Alternate Names

41BB, CD137, ILA, TNFRSF9

Entrez Gene IDs

3604 (Human); 21942 (Mouse); 500590 (Rat); 102127961 (Cynomolgus Monkey)

Gene Symbol

TNFRSF9

UniProt

P20334

Additional 4-1BB/TNFRSF9/CD137 Products

Product Documents for Mouse 4‑1BB/TNFRSF9/CD137 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse 4‑1BB/TNFRSF9/CD137 Antibody

For research use only

Citations for Mouse 4‑1BB/TNFRSF9/CD137 Antibody

Customer Reviews for Mouse 4‑1BB/TNFRSF9/CD137 Antibody (4)

5 out of 5
4 Customer Ratings
5 Stars
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4 Stars
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1 Stars
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Showing  1 - 4 of 4 reviews Showing All
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  • Mouse 4-1BB/TNFRSF9/CD137 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: M41BB antigen
    Species: Mouse
    Verified Customer | Posted 04/10/2019
    Mouse 4‑1BB/TNFRSF9/CD137 Antibody MAB9371
  • Mouse 4-1BB/TNFRSF9/CD137 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: COMMERCIAL ANTIGEN and M41BB ANTIGEN
    Species: Mouse
    Verified Customer | Posted 04/07/2019
    Mouse 4‑1BB/TNFRSF9/CD137 Antibody MAB9371
  • Mouse 4-1BB/TNFRSF9/CD137 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Commercial Antigen
    Species: Mouse
    Verified Customer | Posted 12/16/2018
    Mouse 4‑1BB/TNFRSF9/CD137 Antibody MAB9371
  • Mouse 4-1BB/TNFRSF9/CD137 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: commercial Antigen
    Species: Mouse
    Verified Customer | Posted 10/16/2018
    Mouse 4‑1BB/TNFRSF9/CD137 Antibody MAB9371

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