Detection of Mouse Afamin by Western Blot.
Western blot shows mouse serum. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Mouse Afamin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8119) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Afamin at approximately 87 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Afamin, also known as AFM or alpha -Albumin,
is an 84-88 kDa secreted monomeric glycoprotein member of the
Alb/albumin family of molecules. Expressed by hepatocytes, CNS
endothelial cells and osteoclasts, Afamin circulates in the blood at
low μg/mL concentrations. Afamin is known to bind and transport
vitamin E, particularly under conditions where lipoprotein is
limited. This is likely to be important in follicular fluid and CSF.
Afamin also serves as an osteoclast-derived chemoattractant for
preosteoblasts, providing a rationale for the observation that bone
formation often follows bone resorption. Mature mouse Afamin is 587
amino acids (aa) in length (aa 22-608). It contains three
consecutive albumin domains (aa 22-210, 211-403 and 404-599) that
contain characteristic 5 or 6 intrachain disulfide bonds.
Full-length mouse Afamin shares 67% aa sequence identity with human
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