Mouse AgRP/ART Antibody
Mouse AgRP/ART Antibody Summary
Val25-Thr131
Accession # P56473
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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AgRP/ART in Mouse Brain. AgRP/ART was detected in perfusion fixed frozen sections of mouse brain (hypothalamus) using Goat Anti-Mouse AgRP/ART Antigen Affinity-purified Polyclonal Antibody (Catalog # AF634) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
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Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: AgRP/ART
Agouti-Related Protein (AgRP), the protein product of the Agouti-Related Transcript (ART), is a neuroprotein that regulates energy metabolism and the development of obesity by antagonizing alpha -melanocyte stimulating hormone ( alpha -MSH) action on MC-3 and MC-4 receptors (1-4). AgRP is predominantly expressed in the hypothalamus and adrenal medulla (5). Mature mouse AgRP is a 111 amino acid (aa) polypeptide; its C-terminal portion contains ten conserved cysteines that form five disulfide bonds (5, 6). Within the C-terminal region, mouse AgRP shares 80% and 90% aa sequence identity with human and rat AgRP, respectively. It also shares 44% aa sequence identity with Agouti. As with Agouti, the C-terminal cysteine-rich region is sufficient for biological activity (7). AgRP, however, is 100 times more potent than Agouti in antagonizing MC-3 and MC-4 receptors (8). AgRP also induces the beta -arrestin dependent endocytosis of MC-3 and MC-4 (9). Hypothalamic expression of AgRP is upregulated in obesity and diabetes (5, 10), and chronic AgRP administration increases food intake and weight gain in rats (11). Genetically-linked polymorphisms of AgRP in humans are associated with susceptibility to anorexia nervosa (12, 13). In addition, AgRP inhibits the ACTH-induced synthesis of steroid hormones via a mechanism that does not involve melanocortin receptors (14).
- Martin, N.M. et al. (2006) Peptides 27:333.
- Fan, W. et al. (1997) Nature 385:165.
- Ollmann, M.M. et al. (1997) Science 278:135.
- Arora, S. and Anubhuti (2006) Neuropeptides 40:375.
- Shutter, J.R. et al. (1997) Genes Dev. 11:593.
- Kiefer, L.L. et al. (1998) Biochemistry 37:991.
- Jackson, P.J. et al. (2002) Biochemistry 41:7565.
- Fong, T.M. et al. (1997) Biochem. Biophys. Res. Commun. 237:629.
- Breit, A. et al. (2006) J. Biol. Chem. 281:37447.
- Katsuki, A. et al. (2001) J. Clin. Endocrinol. Metab. 86:1921.
- Small, C.K. et al. (2001) Diabetes 50:248.
- Vink, T. et al. (2001) Mol. Psychiatry 6:325.
- Dardennes, R.M. et al. (2007) Psychoneuroendocrinology 32:106.
- Doghman, M. et al. (2007) Mol. Cell. Endocrinol. 265-266:108.
Product Datasheets
Citations for Mouse AgRP/ART Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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High-fat diet accelerates extreme obesity with hyperphagia in female heterozygous Mecp2-null mice
Authors: S Fukuhara, H Nakajima, S Sugimoto, K Kodo, K Shigehara, H Morimoto, Y Tsuma, M Moroto, J Mori, K Kosaka, M Morimoto, H Hosoi
PLoS ONE, 2019;14(1):e0210184.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
A GPR17-cAMP-Lactate Signaling Axis in Oligodendrocytes Regulates Whole-Body Metabolism
Authors: Z Ou, Y Ma, Y Sun, G Zheng, S Wang, R Xing, X Chen, Y Han, J Wang, QR Lu, TJ Zhao, Y Chen
Cell Rep, 2019;26(11):2984-2997.e4.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Tanycytic TSPO inhibition induces lipophagy to regulate lipid metabolism and improve energy balance
Authors: S Kim, N Kim, S Park, Y Jeon, J Lee, SJ Yoo, JW Lee, C Moon, SW Yu, EK Kim
Autophagy, 2019;0(0):1-21.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
QRFP-Deficient Mice Are Hypophagic, Lean, Hypoactive and Exhibit Increased Anxiety-Like Behavior
PLoS ONE, 2016;11(11):e0164716.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
MCT2 expression and lactate influx in anorexigenic and orexigenic neurons of the arcuate nucleus.
Authors: Cortes-Campos C, Elizondo R, Carril C, Martinez F, Boric K, Nualart F, Garcia-Robles M
PLoS ONE, 2013;8(4):e62532.
Species: Rat
Sample Types: Whole Cells
Applications: ICC
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