Detects mouse alpha 2-Macroglobulin in direct ELISAs and Western blots. In direct ELISA, less than 2% cross-reactivity with human alpha 2‑Macroglobulin, recombinant rat (rr) alpha 2-Macroglobulin, and rr beta 2-Macroglobulin is observed.
Polyclonal Sheep IgG
Mouse serum-derived alpha 2-Macroglobulin
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Mouse alpha 2-Macroglobulin by Western Blot. Western blot shows samples of purified alpha 2-Macroglobulin from mouse sera. PVDF membrane was probed with 0.1 µg/mL Sheep Anti-Mouse alpha 2-Macroglobulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5798) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for alpha 2-Macroglobulin was detected at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha 2-Macroglobulin
alpha 2-Macroglobulin (alpha 2 macroglobulin; also A2M and MAM) is a 185 kDa member of the protease inhibitor I39 family of proteins. It is secreted by the liver, circulates at a constant level in the blood, binds multiple growth factors, inhibits a wide variety of proteinases via steric trapping, is cleared by cell surface receptors and likely exists as a tetramer. Mature mouse A2M is 1471 amino acids (aa) in length. It is cleaved between Arg1239 and Asp1240 to generate two subunits, one that is 165 kDa in size and 1215 aa in length and a second that is 35 kDa in size and 256 aa in length. The protease inhibitor region (aa 686-744) is on the large subunit, while the receptor binding segment (aa 1395-1482) is on the smaller subunit. A2M has a counterpart gene termed A2M-P that is associated with pregnancy. The A2M-P molecule shares 56% aa identity with mouse A2M. Notably, mouse A2M-P is closer in aa sequence to human A2M (72%) and rat A2M (91%) than mouse A2M is to human A2M (59%) and rat A2M (56%).
Entrez Gene IDs:
A2M; alpha 2Macroglobulin; alpha-2-M; alpha-2-macroglobulin; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5; CPAMD5; CPAMD5DKFZp779B086; FWP007; S863-7
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