Mouse alpha-Fetoprotein/AFP Quantikine ELISA Kit Summary
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
|Intra-Assay Precision||Inter-Assay Precision|
The recovery of mouse AFP spiked to levels throughout the range of the assay was evaluated.
|Sample Type||Average % Recovery||Range %|
|Cell Culture Media (n=4)||98||93-105|
Mouse alpha-Fetoprotein/AFP Standard Curve
Preparation and Storage
alpha-Fetoprotein (AFP), is albuminoid superfamily protein that is synthesized in the fetus primarily by the liver, yolk sac, and tissues of gastrointestinal origin. It is one of the earliest markers of the hepatocyte lineage. AFP acts as a carrier protein for steroids, bilirubin, fatty acids, retinoids, and flavonoids. In addition, it can exert immunosuppressive activity, regulate cell proliferation and apoptosis, initiate intracellular signaling, and contribute to cell invasion. Altered levels of both fetal and maternal AFP have been associated with hypothyroidism, autoimmune disorders, and heart defects. Low maternal serum AFP levels are associated with a higher incidence of Down syndrome, whereas higher levels are associated with spina bifida and anencephaly. Elevated AFP levels are also coincident with liver, stomach, and germ cell cancers.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 4 times.
- Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations for Mouse alpha-Fetoprotein/AFP Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
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Deficiency of fibroblast growth factor 21 (FGF21) promotes hepatocellular carcinoma (HCC) in mice on a long term obesogenic diet
Authors: G Singhal, G Kumar, S Chan, FM Fisher, Y Ma, HG Vardeh, IA Nasser, JS Flier, E Maratos-Fl
Mol Metab, 2018;0(0):.
Sample Types: Serum
Up-regulation of FGF15/19 signaling promotes hepatocellular carcinoma in the background of fatty liver
Authors: G Cui, RC Martin, H Jin, X Liu, H Pandit, H Zhao, L Cai, P Zhang, W Li, Y Li
J. Exp. Clin. Cancer Res., 2018;37(1):136.
Sample Types: Plasma
Adult mouse model of early hepatocellular carcinoma promoted by alcoholic liver disease
Authors: A Ambade, A Satishchan, B Gyongyosi, P Lowe, G Szabo
World J Gastroenterol, 2016;22(16):4091-108.
Sample Types: Serum
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Average Rating: 4.8 (Based on 4 Reviews)
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Easy to use with reliable results. Would recommend.