Detection of Mouse alpha 1-Acid Glycoprotein by Western Blot. Western blot shows lysates of mouse liver tissue. PVDF membrane was probed with 1 µg/mL of Mouse alpha 1‑Acid Glycoprotein Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5934) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for alpha 1‑Acid Glycoprotein at approximately 43kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha 1-Acid Glycoprotein
alpha 1-Acid Glycoprotein (AGP; also OMD/Orosomucoid) is a 40‑46 kDa member of the immunocalin subfamily, lipocalin family of molecules. In mouse, circulating AGP is principally the product of hepatocytes that originates from multiple related genes (AGP-1, -2 & -3 in Mus musculus). Circulating AGP-1 and -2 are both 189 amino acids (aa) in length, the principal sources of protein, and show 83% aa identity; AGP-3 contributes little to the AGP pool. In mouse blood, AGP is normally 200‑400 μg/mL. In response to inflammatory mediators (IL-6; IL-1), its concentration will rise 2 to 10 fold. More importantly, a complex glycosylation pattern will also change, transitioning from modestly branched to highly branched oligosaccharides. This change is reflected in its bioactivity, which has been shown to be a function of carbohydrate branching. AGP is generally considered to be a suppressor of inflammation. Rat and human AGP share only 70% and 47% aa identity with mouse AGP, respectively.
Have you used Mouse alpha 1-Acid Glycoprotein Antibody?
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.