Mouse Amphiregulin Antibody

(8 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Amphiregulin in ELISAs and Western Blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant human Amphiregulin is observed. In Western blots, no cross reactivity with recombinant mouse HB-EGF is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse Amphiregulin
    Ser94-Lys191
    Accession # P31955
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
    • Mouse Amphiregulin Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    0.2-0.8 µg/mL 
    Mouse Amphiregulin Antibody (Catalog # AF989)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse Amphiregulin Biotinylated Antibody (Catalog # BAF989)
  • ELISA Standard
     
    Recombinant Mouse Amphiregulin Protein (Catalog # 989-AR)
  • Neutralization
    Measured by its ability to neutralize Amphiregulin-induced proliferation in the Balb/3T3 mouse embryonic fibroblast cell line. The Neutralization Dose (ND50) is typically 0.3-1.5 µg/mL in the presence of 50 ng/mL Recombinant Mouse Amphiregulin.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Recombinant Human and Mouse Amphiregulin by Western Blot. Western blot shows 25 ng of Recombinant Mouse Amphiregulin (Catalog # 989-AR), Recombinant Human Amphiregulin (Catalog # 262-AR), and Recombinant Mouse HB-EGF. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse Amphiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF989) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Amphiregulin at approximately 16-25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Immunohistochemistry
Amphiregulin in Mouse Skin. Amphiregulin was detected in immersion fixed frozen sections of mouse skin using Goat Anti-Mouse Amphiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF989) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to keratinocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Cell Proliferation Induced by Amphiregulin and Neutralization by Mouse Amphiregulin Antibody. Recombinant Mouse Amphiregulin (Catalog # 989‑AR) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Amphiregulin (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Amphiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF989). The ND50 is typically 0.3-1.5 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Amphiregulin

Amphiregulin (AR), also known as Schwannoma-derived growth factor (SDGF), is a member of the epidermal growth factor (EGF) family of growth factors which includes, AR, EGF, transforming growth factor-a (TGF-a), heparin binding EGF-like growth factor (HB-EGF), betacellulin (BTC), epiregulin, and the neuregulins-1 through -4. All EGF family members are synthesized as type I transmembrane precursors and contain one or several EGF domains in their extracellular region. The bioactive form of the proteins is released by proteolytic cleavage. The ErbB family of receptors that includes ErbB1‑B4, mediates the biological activities of the EGF family ligands. AR was originally isolated from the conditioned media of PMA-treated MCF-7 human breast carcinoma cell line. AR mRNA expression can be detected in numerous carcinoma cell lines and in the epithelial cells of various human tissues including colon, stomach, breast, ovary and kidney. AR stimulates the proliferation of keratinocytes, mammary epithelial cells, fibroblasts, astrocytes and glial cells. AR is also a growth inhibitor for certain tumor cells. The gene for AR has been mapped to human chromosome 4q13-q21 and mouse chromosome 5. Human and mouse AR cDNA encode 252 and 248 amino acid residue type I membrane proteins, respectively. The two proteins share approximately 69% sequence identity. Mouse AR also shares 81% amino acid sequence homology with rat AR. Several secreted isoforms of AR that vary in length and/or glycosylation level can be found in cell conditioned media. The 98 amino acid residue recombinant AR has better receptor binding and biological activity than the C-terminal truncated forms of the protein.

  • References:
    1. Thompson, S.A. et al. (1996) J. Biol. Chem. 271:17927.
    2. Sonoda, H. et al. (1992) Biochem. Biophys. Res. Commun. 185:103.
    3. Normanno, N. et al. (2001) Frontiers in Bioscience 6:685.
  • Entrez Gene IDs:
    374 (Human); 11839 (Mouse)
  • Alternate Names:
    Amphiregulin; AR; AREG; AREGB; Colorectum cell-derived growth factor; CRDGF; MGC13647; schwannoma-derived growth factor; SDGF
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Species
Applications
Sample Type
  1. A heart-brain-kidney network controls adaptation to cardiac stress through tissue macrophage activation
    Authors: K Fujiu, M Shibata, Y Nakayama, F Ogata, S Matsumoto, K Noshita, S Iwami, S Nakae, I Komuro, R Nagai, I Manabe
    Nat. Med., 2017;23(5):611-622.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  2. Amphiregulin Antibody and Reduction of Axial Elongation in Experimental Myopia
    Authors: WJ Jiang, HX Song, SY Li, B Guo, JF Wu, GP Li, DD Guo, L Shi, HS Bi, JB Jonas
    EBioMedicine, 2017;0(0):.
    Species: Guinea Pig
    Sample Type: In Vivo
    Application: In vivo
  3. Amphiregulin contained in NSCLC-exosomes induces osteoclast differentiation through the activation of EGFR pathway
    Authors: S Taverna, M Pucci, M Giallombar, MA Di Bella, M Santarpia, P Reclusa, I Gil-Bazo, C Rolfo, R Alessandro
    Sci Rep, 2017;7(1):3170.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  4. ZEB1 sensitizes lung adenocarcinoma to metastasis suppression by PI3K antagonism.
    Authors: Yang Y, Ahn Y, Chen Y, Tan X, Guo L, Gibbons D, Ungewiss C, Peng D, Liu X, Lin S, Thilaganathan N, Wistuba I, Rodriguez-Canales J, McLendon G, Creighton C, Kurie J
    J Clin Invest, 2014;124(6):2696-708.
    Species: Mouse
    Sample Type: Protein
    Application: WB
  5. Pubertal exposure to high fat diet causes mouse strain-dependent alterations in mammary gland development and estrogen responsiveness.
    Authors: Olson LK, Tan Y, Zhao Y, Aupperlee MD, Haslam SZ
    Int J Obes (Lond), 2010;34(9):1415-26.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  6. The Arf-inducing transcription factor Dmp1 encodes a transcriptional activator of amphiregulin, thrombospondin-1, JunB and Egr1.
    Authors: Mallakin A, Sugiyama T, Kai F, Taneja P, Kendig RD, Frazier DP, Maglic D, Matise LA, Willingham MC, Inoue K
    Int. J. Cancer, 2010;126(6):1403-16.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  7. Ectodomain shedding of EGFR ligands and TNFR1 dictates hepatocyte apoptosis during fulminant hepatitis in mice.
    Authors: Murthy A, Defamie V, Smookler DS
    J. Clin. Invest., 2010;120(8):2731-44.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  8. Wounding-induced synthesis of hyaluronic acid in organotypic epidermal cultures requires the release of heparin-binding egf and activation of the EGFR.
    Authors: Monslow J, Sato N, Mack JA, Maytin EV
    J. Invest. Dermatol., 2009;129(8):2046-58.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
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Normal Goat IgG Control

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Secondary Antibodies
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