Mouse Amphiregulin Antibody [Biotin]

R&D Systems | Catalog # BAF989

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Rat, Transgenic Mouse

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair)

Cited:

Immunohistochemistry, Western Blot, Flow Cytometry, FACS

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all Amphiregulin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Amphiregulin
Ser94-Lys191
Accession # Q4FJT2

Specificity

Detects mouse Amphiregulin in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant human Amphiregulin is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Amphiregulin Antibody [Biotin]

Mouse Amphiregulin ELISA Standard Curve

Mouse Amphiregulin ELISA Standard Curve

Recombinant Mouse Amphiregulin (Catalog # 989-AR) was serially diluted and captured by Goat Anti-Mouse Amphiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF989) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Amphiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF989) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Mouse Amphiregulin Antibody [Biotin]

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Amphiregulin (Catalog # 989-AR)

Mouse Amphiregulin Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Mouse Amphiregulin Antibody (Catalog # AF989)
  • Standard: Recombinant Mouse Amphiregulin Protein (Catalog # 989-AR)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4.5 using BAF989 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Amphiregulin

Amphiregulin (AR), also known as Schwannoma-derived growth factor (SDGF), is a member of the epidermal growth factor (EGF) family of growth factors which includes, AR, EGF, transforming growth factor-a (TGF-a), heparin binding EGF-like growth factor (HB-EGF), betacellulin (BTC), epiregulin, and the neuregulins-1 through -4. All EGF family members are synthesized as type I transmembrane precursors and contain one or several EGF domains in their extracellular region. The bioactive form of the proteins is released by proteolytic cleavage. The ErbB family of receptors that includes ErbB1‑B4, mediates the biological activities of the EGF family ligands. AR was originally isolated from the conditioned media of PMA-treated MCF-7 human breast carcinoma cell line. AR mRNA expression can be detected in numerous carcinoma cell lines and in the epithelial cells of various human tissues including colon, stomach, breast, ovary and kidney. AR stimulates the proliferation of keratinocytes, mammary epithelial cells, fibroblasts, astrocytes and glial cells. AR is also a growth inhibitor for certain tumor cells. The gene for AR has been mapped to human chromosome 4q13-q21 and mouse chromosome 5. Human and mouse AR cDNA encode 252 and 248 amino acid residue type I membrane proteins, respectively. The two proteins share approximately 69% sequence identity. Mouse AR also shares 81% amino acid sequence homology with rat AR. Several secreted isoforms of AR that vary in length and/or glycosylation level can be found in cell conditioned media. The 98 amino acid residue recombinant AR has better receptor binding and biological activity than the C-terminal truncated forms of the protein.

References

  1. Thompson, S.A. et al. (1996) J. Biol. Chem. 271:17927.
  2. Sonoda, H. et al. (1992) Biochem. Biophys. Res. Commun. 185:103.
  3. Normanno, N. et al. (2001) Frontiers in Bioscience 6:685.

Alternate Names

AREG

Entrez Gene IDs

374 (Human); 11839 (Mouse)

Gene Symbol

AREG

UniProt

Q4FJT2

Additional Amphiregulin Products

Product Documents for Mouse Amphiregulin Antibody [Biotin]

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Amphiregulin Antibody [Biotin]

For research use only

Related Research Areas

EGF Family

Citations for Mouse Amphiregulin Antibody [Biotin]

Customer Reviews for Mouse Amphiregulin Antibody [Biotin] (2)

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2 Customer Ratings
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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • Mouse Amphiregulin Biotinylated Antibody
    Name: Oliver Burton
    Application: Flow Cytometry
    Sample Tested: Mouse splenocytes
    Species: Mouse
    Verified Customer | Posted 09/12/2017
    Splenocytes (2x10^6) from a C57BL/6 mouse were stimulated for 4 hours with phorbol 12,13-dibutyrate (500ng/ml), ionomycin (750ng/ml) and brefeldin A (2ug/ml). Cells were stained with fixable viability dye, then fixed with 2% formalin, permeabilized, and stained overnight at 4C for cytokines and gating markers (CD3, CD4, Foxp3). The biotinylated amphiregulin antibody was used at a 1:500 dilution overnight in permeabilization buffer. The cells were washed twice, then incubated with Brilliant Violet 605 streptavidin at a 1:1000 dilution for 20min. Foxp3+ Tregs were chosen as a positive control. Other appropriate positive controls would be Th2 cells or ILC2 (e.g., gut lamina propria leukocytes). Unstimulated cells that were otherwise treated identically served as a negative control. Flow diagrams are shown gating on viable CD3+CD4+ T cells.
    Mouse Amphiregulin Antibody [Biotin] BAF989
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID: 24315098
    Species: Mouse
    Verified Customer | Posted 01/15/2015

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FAQs

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Associated Pathways

Th2 Differentiation Pathway
Th2 Differentiation Pathway Thumbnail